Amikacin Induces Renal Morphohistological Alterations in Wistar Rats
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001 |
Resumo: | The aim of this experimental study was to investigate the morphohistological effects of amikacin in rats. Male Wistar rats aged 8-12 weeks and weighing 200-250g were placed in a temperature (23±2°C) controlled room in which a 12/12h light: dark cycle was maintained. Twenty-five rats were randomly assigned to five experimental groups of five animals each that received daily treatment with 15 mg/kg of amikacin intraperitoneally. The control group was treated with 0.9% saline for 28 days. Groups I, II, III and IV were injected intaperitoneally with amikacin for 7, 14, 21 and 28 days, respectively. Renal tissues slides were stained with PAS reagent and examined light microscopy. The morphology of the PAS stained structures of the renal glomeruli was evaluated in the different experimental groups using scores. The renal morphohistology and the morphologic analysis of PAS staining were analysed statistically with the Kruskal-Wallis Test and Dunn’s Multiple Comparisons Test (5% statistical significance). The results show microscopically that kidneys in the groups treated with amikacin for 3 and 4 weeks showed marked cellular necrosis (p< 0.0001), tubular obstruction, and basal membrane interruption. This result clearly shows that amikacin administration induces renal morphohistological alterations. |
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Amikacin Induces Renal Morphohistological Alterations in Wistar Ratsamikacinaminoglycosidesnephrotoxicitytubular injuryThe aim of this experimental study was to investigate the morphohistological effects of amikacin in rats. Male Wistar rats aged 8-12 weeks and weighing 200-250g were placed in a temperature (23±2°C) controlled room in which a 12/12h light: dark cycle was maintained. Twenty-five rats were randomly assigned to five experimental groups of five animals each that received daily treatment with 15 mg/kg of amikacin intraperitoneally. The control group was treated with 0.9% saline for 28 days. Groups I, II, III and IV were injected intaperitoneally with amikacin for 7, 14, 21 and 28 days, respectively. Renal tissues slides were stained with PAS reagent and examined light microscopy. The morphology of the PAS stained structures of the renal glomeruli was evaluated in the different experimental groups using scores. The renal morphohistology and the morphologic analysis of PAS staining were analysed statistically with the Kruskal-Wallis Test and Dunn’s Multiple Comparisons Test (5% statistical significance). The results show microscopically that kidneys in the groups treated with amikacin for 3 and 4 weeks showed marked cellular necrosis (p< 0.0001), tubular obstruction, and basal membrane interruption. This result clearly shows that amikacin administration induces renal morphohistological alterations.ArquiMed - Edições Científicas AEFMUP2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001Arquivos de Medicina v.23 n.6 2009reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001Sousa,Vanessa Barboza deDutra,Ilana Jozi PereiraLucena,Hévio Freitas deMedeiros,Hilkéia Carla de SouzaGuerra,Gerlane Coelho BernardoAraújo Júnior,Raimundo F. deAraújo,Aurigena A.Alves,Maria do Socorro Costa Feitosainfo:eu-repo/semantics/openAccess2024-02-06T17:03:24Zoai:scielo:S0871-34132009000600001Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:18:05.351682Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
title |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
spellingShingle |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats Sousa,Vanessa Barboza de amikacin aminoglycosides nephrotoxicity tubular injury |
title_short |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
title_full |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
title_fullStr |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
title_full_unstemmed |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
title_sort |
Amikacin Induces Renal Morphohistological Alterations in Wistar Rats |
author |
Sousa,Vanessa Barboza de |
author_facet |
Sousa,Vanessa Barboza de Dutra,Ilana Jozi Pereira Lucena,Hévio Freitas de Medeiros,Hilkéia Carla de Souza Guerra,Gerlane Coelho Bernardo Araújo Júnior,Raimundo F. de Araújo,Aurigena A. Alves,Maria do Socorro Costa Feitosa |
author_role |
author |
author2 |
Dutra,Ilana Jozi Pereira Lucena,Hévio Freitas de Medeiros,Hilkéia Carla de Souza Guerra,Gerlane Coelho Bernardo Araújo Júnior,Raimundo F. de Araújo,Aurigena A. Alves,Maria do Socorro Costa Feitosa |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa,Vanessa Barboza de Dutra,Ilana Jozi Pereira Lucena,Hévio Freitas de Medeiros,Hilkéia Carla de Souza Guerra,Gerlane Coelho Bernardo Araújo Júnior,Raimundo F. de Araújo,Aurigena A. Alves,Maria do Socorro Costa Feitosa |
dc.subject.por.fl_str_mv |
amikacin aminoglycosides nephrotoxicity tubular injury |
topic |
amikacin aminoglycosides nephrotoxicity tubular injury |
description |
The aim of this experimental study was to investigate the morphohistological effects of amikacin in rats. Male Wistar rats aged 8-12 weeks and weighing 200-250g were placed in a temperature (23±2°C) controlled room in which a 12/12h light: dark cycle was maintained. Twenty-five rats were randomly assigned to five experimental groups of five animals each that received daily treatment with 15 mg/kg of amikacin intraperitoneally. The control group was treated with 0.9% saline for 28 days. Groups I, II, III and IV were injected intaperitoneally with amikacin for 7, 14, 21 and 28 days, respectively. Renal tissues slides were stained with PAS reagent and examined light microscopy. The morphology of the PAS stained structures of the renal glomeruli was evaluated in the different experimental groups using scores. The renal morphohistology and the morphologic analysis of PAS staining were analysed statistically with the Kruskal-Wallis Test and Dunn’s Multiple Comparisons Test (5% statistical significance). The results show microscopically that kidneys in the groups treated with amikacin for 3 and 4 weeks showed marked cellular necrosis (p< 0.0001), tubular obstruction, and basal membrane interruption. This result clearly shows that amikacin administration induces renal morphohistological alterations. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-34132009000600001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ArquiMed - Edições Científicas AEFMUP |
publisher.none.fl_str_mv |
ArquiMed - Edições Científicas AEFMUP |
dc.source.none.fl_str_mv |
Arquivos de Medicina v.23 n.6 2009 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799137272678842368 |