The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/24804 |
Resumo: | Renal cell carcinoma (RCC) is the most common and most lethal urological neoplasia amongst adults. In fact, approximately one-third of the patients are diagnosed with metastatic disease and approximately 20-30% will develop metastasis even after surgical nephrectomy. It is known that EVs play a crucial role in the tumour microenvironment establishment and are involved in the metastatic process. Thus, the aim of this study was to analyze an EV-derived miRNA profile associated with clear cell RCC (ccRCC) development and its role on the cell phenotype modulation. We analyzed a five-miRNA profile in EVs derived from tumoural RCC cell lines (tumoural vs normal) that represented different stages of ccRCC progression. We observed an increase in hsa-miR-571 and hsa-miR-3074-3p levels in EVs derived from metastatic cell line, FG-2, when compared to the control which led to a decrease in the RAD51mRNA expression levels. Additionally, we verified that the administration of EVs-FG-2 to the normal cell line led to a decreased cellular proliferation and migration of the recipient cell line. In conclusion, EVs-FG-2 carry hsa-miR-571 and hsa-miR-3074-3p acting as suppressors of tumour development. |
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The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinomaRenal cell carcinomaRCCExtracellular vesiclesEVsEV-derived mirRNAsRenal cell carcinoma (RCC) is the most common and most lethal urological neoplasia amongst adults. In fact, approximately one-third of the patients are diagnosed with metastatic disease and approximately 20-30% will develop metastasis even after surgical nephrectomy. It is known that EVs play a crucial role in the tumour microenvironment establishment and are involved in the metastatic process. Thus, the aim of this study was to analyze an EV-derived miRNA profile associated with clear cell RCC (ccRCC) development and its role on the cell phenotype modulation. We analyzed a five-miRNA profile in EVs derived from tumoural RCC cell lines (tumoural vs normal) that represented different stages of ccRCC progression. We observed an increase in hsa-miR-571 and hsa-miR-3074-3p levels in EVs derived from metastatic cell line, FG-2, when compared to the control which led to a decrease in the RAD51mRNA expression levels. Additionally, we verified that the administration of EVs-FG-2 to the normal cell line led to a decreased cellular proliferation and migration of the recipient cell line. In conclusion, EVs-FG-2 carry hsa-miR-571 and hsa-miR-3074-3p acting as suppressors of tumour development.Teixeira, Ana Luísa PereiraSantos, Marlene Elisabete MoreiraRepositório Científico do Instituto Politécnico do PortoVenâncio, Patrícia Sofia Almeida2023-11-082024-05-08T00:00:00Z2023-11-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/24804TID:203474732enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T01:51:23Zoai:recipp.ipp.pt:10400.22/24804Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:59:09.977545Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
title |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
spellingShingle |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma Venâncio, Patrícia Sofia Almeida Renal cell carcinoma RCC Extracellular vesicles EVs EV-derived mirRNAs |
title_short |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
title_full |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
title_fullStr |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
title_full_unstemmed |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
title_sort |
The role of extracellular vesicles-derived microRNAs in the development of renal cell carcinoma |
author |
Venâncio, Patrícia Sofia Almeida |
author_facet |
Venâncio, Patrícia Sofia Almeida |
author_role |
author |
dc.contributor.none.fl_str_mv |
Teixeira, Ana Luísa Pereira Santos, Marlene Elisabete Moreira Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Venâncio, Patrícia Sofia Almeida |
dc.subject.por.fl_str_mv |
Renal cell carcinoma RCC Extracellular vesicles EVs EV-derived mirRNAs |
topic |
Renal cell carcinoma RCC Extracellular vesicles EVs EV-derived mirRNAs |
description |
Renal cell carcinoma (RCC) is the most common and most lethal urological neoplasia amongst adults. In fact, approximately one-third of the patients are diagnosed with metastatic disease and approximately 20-30% will develop metastasis even after surgical nephrectomy. It is known that EVs play a crucial role in the tumour microenvironment establishment and are involved in the metastatic process. Thus, the aim of this study was to analyze an EV-derived miRNA profile associated with clear cell RCC (ccRCC) development and its role on the cell phenotype modulation. We analyzed a five-miRNA profile in EVs derived from tumoural RCC cell lines (tumoural vs normal) that represented different stages of ccRCC progression. We observed an increase in hsa-miR-571 and hsa-miR-3074-3p levels in EVs derived from metastatic cell line, FG-2, when compared to the control which led to a decrease in the RAD51mRNA expression levels. Additionally, we verified that the administration of EVs-FG-2 to the normal cell line led to a decreased cellular proliferation and migration of the recipient cell line. In conclusion, EVs-FG-2 carry hsa-miR-571 and hsa-miR-3074-3p acting as suppressors of tumour development. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-08 2023-11-08T00:00:00Z 2024-05-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/24804 TID:203474732 |
url |
http://hdl.handle.net/10400.22/24804 |
identifier_str_mv |
TID:203474732 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137075474202624 |