Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Ana
Data de Publicação: 2023
Outros Autores: Sampaio, Cátia I., Ševčovičová, Andrea, Dias, Alice, Oliveira, Rui Pedro Soares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/87059
Resumo: Cynara cardunculus L. var. scolymus, commonly known as globe artichoke, is known for its medicinal and culinary properties since ancient times. Nowadays, with the increasing demand for healthier foods artichoke has been valorized and worldwide production has increased to meet the increasing demand in consumption of the flower and infusion from leaves. The aim of this work was to characterize the main bioactive compounds obtained from artichoke leaf extracts using simple and sustainable techniques. Among different extraction procedures tested, decoction in water gave extracts particularly rich in chlorogenic acid (1) and cynaropicrin (2) as demonstrated by the NMR spectroscopy analysis. Compounds 1 and 2 were efficiently separated by liquid-liquid extraction, as 1 was only found in the aqueous (Aq) fraction and 2 was selectively isolated in the organic (O) fraction. While both fractions showed in vitro antioxidant activity by scavenging activity, only the O fraction was able to protect Saccharomyces cerevisiae cells from oxidative stress. Interestingly, the O fraction was much more antigenotoxic than Aq fraction, suggesting a highly efficient hydroxyl radical scavenging activity. Besides being simple and avoiding organic solvents, decoction in water provides maximal extraction of 1 and 2 and, presumably, maximal bioactivities.
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spelling Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic propertiesCynara cardunculus L. var. scolymusGlobe artichokeChlorogenic acidCynaropicrinAntioxidant activityDNA damage protectionCynara cardunculus L. var. scolymus, commonly known as globe artichoke, is known for its medicinal and culinary properties since ancient times. Nowadays, with the increasing demand for healthier foods artichoke has been valorized and worldwide production has increased to meet the increasing demand in consumption of the flower and infusion from leaves. The aim of this work was to characterize the main bioactive compounds obtained from artichoke leaf extracts using simple and sustainable techniques. Among different extraction procedures tested, decoction in water gave extracts particularly rich in chlorogenic acid (1) and cynaropicrin (2) as demonstrated by the NMR spectroscopy analysis. Compounds 1 and 2 were efficiently separated by liquid-liquid extraction, as 1 was only found in the aqueous (Aq) fraction and 2 was selectively isolated in the organic (O) fraction. While both fractions showed in vitro antioxidant activity by scavenging activity, only the O fraction was able to protect Saccharomyces cerevisiae cells from oxidative stress. Interestingly, the O fraction was much more antigenotoxic than Aq fraction, suggesting a highly efficient hydroxyl radical scavenging activity. Besides being simple and avoiding organic solvents, decoction in water provides maximal extraction of 1 and 2 and, presumably, maximal bioactivities.This work was supported by national funds through the Fundação para a Ciência e Tecnologia, FCT I.P. [“Contrato-Programa” UIDB/04050/2020]; the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding [UIDB/ 00686/2020]; and by the Operation Program of Integrated Infrastructure [Advancing University Capacity and Competence in Research, Development and Innovation, ITMS2014+: 313021X329, co-financed by the European Regional Development Fund].ElsevierUniversidade do MinhoGonçalves, AnaSampaio, Cátia I.Ševčovičová, AndreaDias, AliceOliveira, Rui Pedro Soares de20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/87059eng1878-818110.1016/j.bcab.2023.102808102808https://www.sciencedirect.com/science/article/pii/S1878818123002098?via%3Dihubinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-28T01:19:58Zoai:repositorium.sdum.uminho.pt:1822/87059Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:26:01.602722Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
title Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
spellingShingle Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
Gonçalves, Ana
Cynara cardunculus L. var. scolymus
Globe artichoke
Chlorogenic acid
Cynaropicrin
Antioxidant activity
DNA damage protection
title_short Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
title_full Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
title_fullStr Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
title_full_unstemmed Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
title_sort Cynaropicrin- and chlorogenic acid-rich extracts easily prepared from Cynara cardunculus var. scolymus: antioxidant and antigenotoxic properties
author Gonçalves, Ana
author_facet Gonçalves, Ana
Sampaio, Cátia I.
Ševčovičová, Andrea
Dias, Alice
Oliveira, Rui Pedro Soares de
author_role author
author2 Sampaio, Cátia I.
Ševčovičová, Andrea
Dias, Alice
Oliveira, Rui Pedro Soares de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, Ana
Sampaio, Cátia I.
Ševčovičová, Andrea
Dias, Alice
Oliveira, Rui Pedro Soares de
dc.subject.por.fl_str_mv Cynara cardunculus L. var. scolymus
Globe artichoke
Chlorogenic acid
Cynaropicrin
Antioxidant activity
DNA damage protection
topic Cynara cardunculus L. var. scolymus
Globe artichoke
Chlorogenic acid
Cynaropicrin
Antioxidant activity
DNA damage protection
description Cynara cardunculus L. var. scolymus, commonly known as globe artichoke, is known for its medicinal and culinary properties since ancient times. Nowadays, with the increasing demand for healthier foods artichoke has been valorized and worldwide production has increased to meet the increasing demand in consumption of the flower and infusion from leaves. The aim of this work was to characterize the main bioactive compounds obtained from artichoke leaf extracts using simple and sustainable techniques. Among different extraction procedures tested, decoction in water gave extracts particularly rich in chlorogenic acid (1) and cynaropicrin (2) as demonstrated by the NMR spectroscopy analysis. Compounds 1 and 2 were efficiently separated by liquid-liquid extraction, as 1 was only found in the aqueous (Aq) fraction and 2 was selectively isolated in the organic (O) fraction. While both fractions showed in vitro antioxidant activity by scavenging activity, only the O fraction was able to protect Saccharomyces cerevisiae cells from oxidative stress. Interestingly, the O fraction was much more antigenotoxic than Aq fraction, suggesting a highly efficient hydroxyl radical scavenging activity. Besides being simple and avoiding organic solvents, decoction in water provides maximal extraction of 1 and 2 and, presumably, maximal bioactivities.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/87059
url https://hdl.handle.net/1822/87059
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1878-8181
10.1016/j.bcab.2023.102808
102808
https://www.sciencedirect.com/science/article/pii/S1878818123002098?via%3Dihub
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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