Effects of glycation by methylglyoxal in saccharomyces cerevisae

Detalhes bibliográficos
Autor(a) principal: Pendão, Ana Sofia Mena Aires
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/47838
Resumo: Mestrado em Bioquímica (Bioquímica Médica) Universidade de Lisboa; Faculdade de Ciências, 2021
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spelling Effects of glycation by methylglyoxal in saccharomyces cerevisaeglicaçãometilglioxalMAGEsstress oxidativoSaccharomyces cerevisiaeTeses de mestrado - 2021Departamento de Química e BioquímicaMestrado em Bioquímica (Bioquímica Médica) Universidade de Lisboa; Faculdade de Ciências, 2021Glycation is the nonenzymatic modification of biomolecules caused by reactive dicarbonyl species. In vivo, the major physiological glycation agent is methylglyoxal. This compound’s formation occurs in all living cells, mainly nonenzymatically from dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate during glycolysis. When glycation occurs on proteins it originates advanced glycation end-products (AGEs), which will change the structure and stability of proteins, consequently impairing their functionality. AGEs tend to accumulate on cells, being associated with ageing and several human diseases, such as diabetes and amyloidotic neuropathies (Alzheimer’s disease, Parkinson’s disease and transthyretin amyloidosis). Hence, there is a growing interest in this post-translational modification. However, little is known about the physiologic effects of glycation, since most studies use in vitro approaches, which do not fully reproduce the in vivo process. Saccharomyces cerevisiae is an ideal model of in vivo glycation, being able to reproduce the effects that happen in higher eukaryotic cells. The in vivo study and use of Saccharomyces cerevisiae cells with different genetic backgrounds, gives us an accurate vision of a wide range of phenotypical and physiological effects caused by glycation. In the present study we show that the glyoxalase system seems to be the major detoxifying mechanism of methylglyoxal, since mutations on this pathway lead to lower growth rates. Moreover, using fluorescence microscopy and flow cytometry we found that glycation is not sufficient to alter cell morphology or its viability. However, the effects of glycation seem to be devastating on mitochondrial activity causing its dysfunction and the accumulation of reactive oxygen species (ROS). These findings provide insight to uncover knowledge of the process of glycation, necessary for better comprehension and advance in the cure and early identification of many human diseases that haunt our society.Cordeiro, Carlos, 1966-Silva, Marta SousaRepositório da Universidade de LisboaPendão, Ana Sofia Mena Aires2021-12-30T01:30:19Z202120202021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/47838TID:202695832enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:01Zoai:repositorio.ul.pt:10451/47838Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:59:48.671047Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of glycation by methylglyoxal in saccharomyces cerevisae
title Effects of glycation by methylglyoxal in saccharomyces cerevisae
spellingShingle Effects of glycation by methylglyoxal in saccharomyces cerevisae
Pendão, Ana Sofia Mena Aires
glicação
metilglioxal
MAGEs
stress oxidativo
Saccharomyces cerevisiae
Teses de mestrado - 2021
Departamento de Química e Bioquímica
title_short Effects of glycation by methylglyoxal in saccharomyces cerevisae
title_full Effects of glycation by methylglyoxal in saccharomyces cerevisae
title_fullStr Effects of glycation by methylglyoxal in saccharomyces cerevisae
title_full_unstemmed Effects of glycation by methylglyoxal in saccharomyces cerevisae
title_sort Effects of glycation by methylglyoxal in saccharomyces cerevisae
author Pendão, Ana Sofia Mena Aires
author_facet Pendão, Ana Sofia Mena Aires
author_role author
dc.contributor.none.fl_str_mv Cordeiro, Carlos, 1966-
Silva, Marta Sousa
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pendão, Ana Sofia Mena Aires
dc.subject.por.fl_str_mv glicação
metilglioxal
MAGEs
stress oxidativo
Saccharomyces cerevisiae
Teses de mestrado - 2021
Departamento de Química e Bioquímica
topic glicação
metilglioxal
MAGEs
stress oxidativo
Saccharomyces cerevisiae
Teses de mestrado - 2021
Departamento de Química e Bioquímica
description Mestrado em Bioquímica (Bioquímica Médica) Universidade de Lisboa; Faculdade de Ciências, 2021
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-12-30T01:30:19Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/47838
TID:202695832
url http://hdl.handle.net/10451/47838
identifier_str_mv TID:202695832
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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