The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/15373 |
Resumo: | Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation. |
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The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic EffectsNatural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.This work was supported by grants of the Fundac¸˜ao de Apoio ao Desenvolvimento do Ensino, Ciˆencia e Tecnologia do Mato Grosso do Sul (FUNDECT), Conselho Nacional de Desenvolvimento Cient´ıfico e Tecnol´ogico (CNPq), and Coordenac¸˜ao de Aperfeic¸oamento de Pessoal de N´ıvel Superior (CAPES). Edson Lucas dos Santos, Carlos Alexandre Carollo, and Edgar J. Paredes-Gamero are recipients of fellowships from Brazilian National Research Council (CNPq), Brazil.Biblioteca Digital do IPBCampos, Jaqueline FerreiraCastro, David Tsuyoshi HiramatsuDamião, Marcio JoséTorquato, Heron F. VieiraParedes-Gamero, Edgar JulianCarollo, Carlos AlexandreEstevinho, Leticia M.Souza, Kely de PicoliSantos, Edson Lucas dos2018-01-31T10:00:00Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15373engCampos, Jaqueline Ferreira; Castro, David Tsuyoshi Hiramatsu; Damiaõ, Marcio José; Torquato, Heron F. Vieira; Paredes-Gamero, Edgar J.; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas (2016). The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects. Oxidative Medicine and Cellular Longevity. ISSN 1942-0900. p. 1-131942-090010.1155/2016/8405957info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:56Zoai:bibliotecadigital.ipb.pt:10198/15373Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:58.871320Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
title |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
spellingShingle |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects Campos, Jaqueline Ferreira |
title_short |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
title_full |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
title_fullStr |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
title_full_unstemmed |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
title_sort |
The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects |
author |
Campos, Jaqueline Ferreira |
author_facet |
Campos, Jaqueline Ferreira Castro, David Tsuyoshi Hiramatsu Damião, Marcio José Torquato, Heron F. Vieira Paredes-Gamero, Edgar Julian Carollo, Carlos Alexandre Estevinho, Leticia M. Souza, Kely de Picoli Santos, Edson Lucas dos |
author_role |
author |
author2 |
Castro, David Tsuyoshi Hiramatsu Damião, Marcio José Torquato, Heron F. Vieira Paredes-Gamero, Edgar Julian Carollo, Carlos Alexandre Estevinho, Leticia M. Souza, Kely de Picoli Santos, Edson Lucas dos |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Campos, Jaqueline Ferreira Castro, David Tsuyoshi Hiramatsu Damião, Marcio José Torquato, Heron F. Vieira Paredes-Gamero, Edgar Julian Carollo, Carlos Alexandre Estevinho, Leticia M. Souza, Kely de Picoli Santos, Edson Lucas dos |
description |
Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2018-01-31T10:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/15373 |
url |
http://hdl.handle.net/10198/15373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Campos, Jaqueline Ferreira; Castro, David Tsuyoshi Hiramatsu; Damiaõ, Marcio José; Torquato, Heron F. Vieira; Paredes-Gamero, Edgar J.; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas (2016). The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects. Oxidative Medicine and Cellular Longevity. ISSN 1942-0900. p. 1-13 1942-0900 10.1155/2016/8405957 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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