Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands

Detalhes bibliográficos
Autor(a) principal: da Moura, Aires Januário Fernandes
Data de Publicação: 2023
Outros Autores: Valadas, Vera, Da Veiga Leal, Silvania, Montalvo Sabino, Eddyson, Sousa, Carla A., Pinto, João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/154352
Resumo: Funding Information: We are grateful to the National Institute of Public Health for the laboratory support in Cape Verde, and to technicians from the Ministry of Health for their assistance in field work. We would like to thank Prof. Paulo Almeida for providing DNA controls of Cx. pipiens and Cx. quinquefasciatus used in the species identification PCR assay. Funding Information: This work was funded by national funds through FCT—Fundação para a Ciência e Tecnologia, I.P., within the framework of the project ARBOMONITOR (PTDC/BIA-OUT/29477/2017. Aires da Moura was funded by the Ph.D. fellowship program of Camões I.P. Publisher Copyright: © 2023, The Author(s).
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spelling Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islandsCape VerdeCulex pipiensCulex tigripesGenotypingMosquitoesWolbachiaRA Public aspects of medicineQR MicrobiologyParasitologyveterinary(all)Infectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: We are grateful to the National Institute of Public Health for the laboratory support in Cape Verde, and to technicians from the Ministry of Health for their assistance in field work. We would like to thank Prof. Paulo Almeida for providing DNA controls of Cx. pipiens and Cx. quinquefasciatus used in the species identification PCR assay. Funding Information: This work was funded by national funds through FCT—Fundação para a Ciência e Tecnologia, I.P., within the framework of the project ARBOMONITOR (PTDC/BIA-OUT/29477/2017. Aires da Moura was funded by the Ph.D. fellowship program of Camões I.P. Publisher Copyright: © 2023, The Author(s).Background: Wolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde. Methods: Mosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR–restriction fragment length polymorphism (RFLP) assay on the ankyrin domain gene pk1. Results: Nine mosquito species were collected, including the major vectors Aedes aegypti, Anopheles arabiensis, Culex pipiens sensu stricto, and Culex quinquefasciatus. Wolbachia was only detected in Cx. pipiens s.s. (100% prevalence), Cx. quinquefasciatus (98.3%), Cx. pipiens/quinquefasciatus hybrids (100%), and Culex tigripes (100%). Based on the results of MLST and wsp hypervariable region typing, Wolbachia from the Cx. pipiens complex was assigned to sequence type 9, wPip clade, and supergroup B. PCR/RFLP analysis revealed three wPip groups in Cape Verde, namely wPip-II, wPip-III, and wPip-IV. wPip-IV was the most prevalent, while wPip-II and wPip-III were found only on Maio and Fogo islands. Wolbachia detected in Cx. tigripes belongs to supergroup B, with no attributed MLST profile, indicating a new strain of Wolbachia in this mosquito species. Conclusions: A high prevalence and diversity of Wolbachia was found in species from the Cx. pipiens complex. This diversity may be related to the mosquito's colonization history on the Cape Verde islands. To the best of our knowledge, this is the first study to detect Wolbachia in Cx. tigripes, which may provide an additional opportunity for biocontrol initiatives. Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNda Moura, Aires Januário FernandesValadas, VeraDa Veiga Leal, SilvaniaMontalvo Sabino, EddysonSousa, Carla A.Pinto, João2023-06-23T22:24:08Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/154352eng1756-3305PURE: 63839441https://doi.org/10.1186/s13071-023-05745-winfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:36:48Zoai:run.unl.pt:10362/154352Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:35.547264Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
title Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
spellingShingle Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
da Moura, Aires Januário Fernandes
Cape Verde
Culex pipiens
Culex tigripes
Genotyping
Mosquitoes
Wolbachia
RA Public aspects of medicine
QR Microbiology
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
title_full Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
title_fullStr Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
title_full_unstemmed Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
title_sort Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
author da Moura, Aires Januário Fernandes
author_facet da Moura, Aires Januário Fernandes
Valadas, Vera
Da Veiga Leal, Silvania
Montalvo Sabino, Eddyson
Sousa, Carla A.
Pinto, João
author_role author
author2 Valadas, Vera
Da Veiga Leal, Silvania
Montalvo Sabino, Eddyson
Sousa, Carla A.
Pinto, João
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv da Moura, Aires Januário Fernandes
Valadas, Vera
Da Veiga Leal, Silvania
Montalvo Sabino, Eddyson
Sousa, Carla A.
Pinto, João
dc.subject.por.fl_str_mv Cape Verde
Culex pipiens
Culex tigripes
Genotyping
Mosquitoes
Wolbachia
RA Public aspects of medicine
QR Microbiology
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Cape Verde
Culex pipiens
Culex tigripes
Genotyping
Mosquitoes
Wolbachia
RA Public aspects of medicine
QR Microbiology
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: We are grateful to the National Institute of Public Health for the laboratory support in Cape Verde, and to technicians from the Ministry of Health for their assistance in field work. We would like to thank Prof. Paulo Almeida for providing DNA controls of Cx. pipiens and Cx. quinquefasciatus used in the species identification PCR assay. Funding Information: This work was funded by national funds through FCT—Fundação para a Ciência e Tecnologia, I.P., within the framework of the project ARBOMONITOR (PTDC/BIA-OUT/29477/2017. Aires da Moura was funded by the Ph.D. fellowship program of Camões I.P. Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-06-23T22:24:08Z
2023-12
2023-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/154352
url http://hdl.handle.net/10362/154352
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1756-3305
PURE: 63839441
https://doi.org/10.1186/s13071-023-05745-w
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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