Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth

Detalhes bibliográficos
Autor(a) principal: Proença, Carina
Data de Publicação: 2023
Outros Autores: Rufino, Ana Teresa, Santos, Isabela, Albuquerque, Hélio M. T., Silva, Artur M. S., Fernandes, Eduarda, Oliveira, José Miguel P. Ferreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/42604
Resumo: Osteosarcoma (OS) is a common childhood sarcoma, and its treatment is hindered by adverse effects, chemoresistance, and recurrence. Interleukin (IL)-6 production by tumors plays a significant role in inflammation, carcinogenesis, and metastasis. This study aimed to investigate the antiproliferative potential of luteolin derivatives in OS and to evaluate interleukin production. MG-63, Saos-2, HOS, and 143B human OS cell lines were incubated with luteolin and eight derivatives containing hydroxy, chlorine, or alkyl substitutions. The cell viability and growth were evaluated in the presence of these compounds. Apoptosis was also examined through the analysis of the Bax expression and caspase-3 activity. Finally, the gossypetin effects were measured regarding the production of proinflammatory cytokines interleukin (IL)-6, IL-1β, and IL-12p70. Our findings show that gossypetin was the most potent compound, with proliferation-suppressing activities that induced a series of critical events, including the inhibition of the cell viability and growth. Apoptosis was associated with enhanced caspase-3 activity and increased Bax expression, indicating the involvement of the intrinsic pathway of apoptosis. Moreover, pre-/co-treatment with gossypetin significantly reduced the autocrine production of proinflammatory cytokines. Further investigation is required; nevertheless, considering the link between inflammation, carcinogenesis, and metastasis in OS, our findings suggest that gossypetin exhibits anti-proliferative and anti-inflammatory properties that are potentially relevant in the clinical context.
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spelling Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growthBone sarcomaChemotherapeutic agentsFlavonoid3,3′,4′,5,7,8-hexahydroxyflavoneApoptosisProinflammatory cytokinesOsteosarcoma (OS) is a common childhood sarcoma, and its treatment is hindered by adverse effects, chemoresistance, and recurrence. Interleukin (IL)-6 production by tumors plays a significant role in inflammation, carcinogenesis, and metastasis. This study aimed to investigate the antiproliferative potential of luteolin derivatives in OS and to evaluate interleukin production. MG-63, Saos-2, HOS, and 143B human OS cell lines were incubated with luteolin and eight derivatives containing hydroxy, chlorine, or alkyl substitutions. The cell viability and growth were evaluated in the presence of these compounds. Apoptosis was also examined through the analysis of the Bax expression and caspase-3 activity. Finally, the gossypetin effects were measured regarding the production of proinflammatory cytokines interleukin (IL)-6, IL-1β, and IL-12p70. Our findings show that gossypetin was the most potent compound, with proliferation-suppressing activities that induced a series of critical events, including the inhibition of the cell viability and growth. Apoptosis was associated with enhanced caspase-3 activity and increased Bax expression, indicating the involvement of the intrinsic pathway of apoptosis. Moreover, pre-/co-treatment with gossypetin significantly reduced the autocrine production of proinflammatory cytokines. Further investigation is required; nevertheless, considering the link between inflammation, carcinogenesis, and metastasis in OS, our findings suggest that gossypetin exhibits anti-proliferative and anti-inflammatory properties that are potentially relevant in the clinical context.MDPI2024-10-21T09:58:05Z2023-09-01T00:00:00Z2023-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/42604eng10.3390/antiox12091744Proença, CarinaRufino, Ana TeresaSantos, IsabelaAlbuquerque, Hélio M. T.Silva, Artur M. S.Fernandes, EduardaOliveira, José Miguel P. Ferreira deinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-10-28T01:45:44Zoai:ria.ua.pt:10773/42604Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-10-28T01:45:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
title Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
spellingShingle Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
Proença, Carina
Bone sarcoma
Chemotherapeutic agents
Flavonoid
3,3′,4′,5,7,8-hexahydroxyflavone
Apoptosis
Proinflammatory cytokines
title_short Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
title_full Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
title_fullStr Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
title_full_unstemmed Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
title_sort Gossypetin is a novel modulator of inflammatory cytokine production and a suppressor of osteosarcoma cell growth
author Proença, Carina
author_facet Proença, Carina
Rufino, Ana Teresa
Santos, Isabela
Albuquerque, Hélio M. T.
Silva, Artur M. S.
Fernandes, Eduarda
Oliveira, José Miguel P. Ferreira de
author_role author
author2 Rufino, Ana Teresa
Santos, Isabela
Albuquerque, Hélio M. T.
Silva, Artur M. S.
Fernandes, Eduarda
Oliveira, José Miguel P. Ferreira de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Proença, Carina
Rufino, Ana Teresa
Santos, Isabela
Albuquerque, Hélio M. T.
Silva, Artur M. S.
Fernandes, Eduarda
Oliveira, José Miguel P. Ferreira de
dc.subject.por.fl_str_mv Bone sarcoma
Chemotherapeutic agents
Flavonoid
3,3′,4′,5,7,8-hexahydroxyflavone
Apoptosis
Proinflammatory cytokines
topic Bone sarcoma
Chemotherapeutic agents
Flavonoid
3,3′,4′,5,7,8-hexahydroxyflavone
Apoptosis
Proinflammatory cytokines
description Osteosarcoma (OS) is a common childhood sarcoma, and its treatment is hindered by adverse effects, chemoresistance, and recurrence. Interleukin (IL)-6 production by tumors plays a significant role in inflammation, carcinogenesis, and metastasis. This study aimed to investigate the antiproliferative potential of luteolin derivatives in OS and to evaluate interleukin production. MG-63, Saos-2, HOS, and 143B human OS cell lines were incubated with luteolin and eight derivatives containing hydroxy, chlorine, or alkyl substitutions. The cell viability and growth were evaluated in the presence of these compounds. Apoptosis was also examined through the analysis of the Bax expression and caspase-3 activity. Finally, the gossypetin effects were measured regarding the production of proinflammatory cytokines interleukin (IL)-6, IL-1β, and IL-12p70. Our findings show that gossypetin was the most potent compound, with proliferation-suppressing activities that induced a series of critical events, including the inhibition of the cell viability and growth. Apoptosis was associated with enhanced caspase-3 activity and increased Bax expression, indicating the involvement of the intrinsic pathway of apoptosis. Moreover, pre-/co-treatment with gossypetin significantly reduced the autocrine production of proinflammatory cytokines. Further investigation is required; nevertheless, considering the link between inflammation, carcinogenesis, and metastasis in OS, our findings suggest that gossypetin exhibits anti-proliferative and anti-inflammatory properties that are potentially relevant in the clinical context.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-01T00:00:00Z
2023-09
2024-10-21T09:58:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/42604
url http://hdl.handle.net/10773/42604
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3390/antiox12091744
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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