Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.8/8412 |
| Resumo: | J.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958). |
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Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approachAdditive manufacturingBone tissue engineeringCell-derived extracellular matrixMesenchymal stem/stromal cellsPolycaprolactone scaffoldsJ.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958).The clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques with cell-derived extracellular matrix (ECM) to generate structurally well-defined bioactive scaffolds for bone tissue engineering (BTE). In this work, highly porous three-dimensional polycaprolactone (PCL) scaffolds with desired size and architecture were fabricated by fused deposition modeling and subsequently decorated with human mesenchymal stem/stromal cell (MSC)-derived ECM produced in situ. The successful deposition of MSC-derived ECM onto PCL scaffolds (PCL-MSC ECM) was confirmed after decellularization using scanning electron microscopy, elemental analysis, and immunofluorescence. The presence of cell derived ECM within the PCL scaffolds significantly enhanced MSC attachment and proliferation, with and without osteogenic supplementation. Additionally, under osteogenic induction, PCL-MSC ECM scaffolds promoted significantly higher calcium deposition and elevated relative expression of bone-specific genes, particularly the gene encoding osteopontin, when compared to pristine scaffolds. Overall, our results demonstrated the favorable effects of combining MSC-derived ECM and AM-based scaffolds on the osteogenic differentiation of MSC, resulting from a closer mimicry of the native bone niche. This strategy is highly promising for the development of novel personalized BTE approaches enabling the fabrication of patient defect-tailored scaffolds with enhanced biological performance and osteoinductive properties.WileyIC-OnlineSilva, João C.Carvalho, Marta S.Udangawa, Ranodhi N.Moura, CarlaCabral, Joaquim M. S.Silva, Cláudia L. daFerreira, Frederico CasteloVashishth, DeepakLinhardt, Robert J.2023-04-19T10:32:40Z2020-01-092020-01-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8412engSilva, J. C., Carvalho, M. S., Udangawa, R. N., Moura, C. S., Cabral, J. M. S., L. da Silva, C., Ferreira, F. C., Vashishth, D., & Linhardt, R. J. (2020). Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(5), 2153–2166. https://doi.org/10.1002/jbm.b.345541552-497310.1002/jbm.b.34554metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-26T18:25:15Zoai:iconline.ipleiria.pt:10400.8/8412Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-26T18:25:15Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| title |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| spellingShingle |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach Silva, João C. Additive manufacturing Bone tissue engineering Cell-derived extracellular matrix Mesenchymal stem/stromal cells Polycaprolactone scaffolds |
| title_short |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| title_full |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| title_fullStr |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| title_full_unstemmed |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| title_sort |
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach |
| author |
Silva, João C. |
| author_facet |
Silva, João C. Carvalho, Marta S. Udangawa, Ranodhi N. Moura, Carla Cabral, Joaquim M. S. Silva, Cláudia L. da Ferreira, Frederico Castelo Vashishth, Deepak Linhardt, Robert J. |
| author_role |
author |
| author2 |
Carvalho, Marta S. Udangawa, Ranodhi N. Moura, Carla Cabral, Joaquim M. S. Silva, Cláudia L. da Ferreira, Frederico Castelo Vashishth, Deepak Linhardt, Robert J. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
IC-Online |
| dc.contributor.author.fl_str_mv |
Silva, João C. Carvalho, Marta S. Udangawa, Ranodhi N. Moura, Carla Cabral, Joaquim M. S. Silva, Cláudia L. da Ferreira, Frederico Castelo Vashishth, Deepak Linhardt, Robert J. |
| dc.subject.por.fl_str_mv |
Additive manufacturing Bone tissue engineering Cell-derived extracellular matrix Mesenchymal stem/stromal cells Polycaprolactone scaffolds |
| topic |
Additive manufacturing Bone tissue engineering Cell-derived extracellular matrix Mesenchymal stem/stromal cells Polycaprolactone scaffolds |
| description |
J.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-01-09 2020-01-09T00:00:00Z 2023-04-19T10:32:40Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/8412 |
| url |
http://hdl.handle.net/10400.8/8412 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Silva, J. C., Carvalho, M. S., Udangawa, R. N., Moura, C. S., Cabral, J. M. S., L. da Silva, C., Ferreira, F. C., Vashishth, D., & Linhardt, R. J. (2020). Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(5), 2153–2166. https://doi.org/10.1002/jbm.b.34554 1552-4973 10.1002/jbm.b.34554 |
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metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
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openAccess |
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application/pdf |
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Wiley |
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Wiley |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817547291035172864 |
