Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach

Detalhes bibliográficos
Autor(a) principal: Silva, João C.
Data de Publicação: 2020
Outros Autores: Carvalho, Marta S., Udangawa, Ranodhi N., Moura, Carla, Cabral, Joaquim M. S., Silva, Cláudia L. da, Ferreira, Frederico Castelo, Vashishth, Deepak, Linhardt, Robert J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8412
Resumo: J.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958).
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spelling Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approachAdditive manufacturingBone tissue engineeringCell-derived extracellular matrixMesenchymal stem/stromal cellsPolycaprolactone scaffoldsJ.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958).The clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques with cell-derived extracellular matrix (ECM) to generate structurally well-defined bioactive scaffolds for bone tissue engineering (BTE). In this work, highly porous three-dimensional polycaprolactone (PCL) scaffolds with desired size and architecture were fabricated by fused deposition modeling and subsequently decorated with human mesenchymal stem/stromal cell (MSC)-derived ECM produced in situ. The successful deposition of MSC-derived ECM onto PCL scaffolds (PCL-MSC ECM) was confirmed after decellularization using scanning electron microscopy, elemental analysis, and immunofluorescence. The presence of cell derived ECM within the PCL scaffolds significantly enhanced MSC attachment and proliferation, with and without osteogenic supplementation. Additionally, under osteogenic induction, PCL-MSC ECM scaffolds promoted significantly higher calcium deposition and elevated relative expression of bone-specific genes, particularly the gene encoding osteopontin, when compared to pristine scaffolds. Overall, our results demonstrated the favorable effects of combining MSC-derived ECM and AM-based scaffolds on the osteogenic differentiation of MSC, resulting from a closer mimicry of the native bone niche. This strategy is highly promising for the development of novel personalized BTE approaches enabling the fabrication of patient defect-tailored scaffolds with enhanced biological performance and osteoinductive properties.WileyIC-OnlineSilva, João C.Carvalho, Marta S.Udangawa, Ranodhi N.Moura, CarlaCabral, Joaquim M. S.Silva, Cláudia L. daFerreira, Frederico CasteloVashishth, DeepakLinhardt, Robert J.2023-04-19T10:32:40Z2020-01-092020-01-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8412engSilva, J. C., Carvalho, M. S., Udangawa, R. N., Moura, C. S., Cabral, J. M. S., L. da Silva, C., Ferreira, F. C., Vashishth, D., & Linhardt, R. J. (2020). Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(5), 2153–2166. https://doi.org/10.1002/jbm.b.345541552-497310.1002/jbm.b.34554metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:57:16Zoai:iconline.ipleiria.pt:10400.8/8412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:08.466333Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
title Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
spellingShingle Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
Silva, João C.
Additive manufacturing
Bone tissue engineering
Cell-derived extracellular matrix
Mesenchymal stem/stromal cells
Polycaprolactone scaffolds
title_short Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
title_full Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
title_fullStr Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
title_full_unstemmed Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
title_sort Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach
author Silva, João C.
author_facet Silva, João C.
Carvalho, Marta S.
Udangawa, Ranodhi N.
Moura, Carla
Cabral, Joaquim M. S.
Silva, Cláudia L. da
Ferreira, Frederico Castelo
Vashishth, Deepak
Linhardt, Robert J.
author_role author
author2 Carvalho, Marta S.
Udangawa, Ranodhi N.
Moura, Carla
Cabral, Joaquim M. S.
Silva, Cláudia L. da
Ferreira, Frederico Castelo
Vashishth, Deepak
Linhardt, Robert J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Silva, João C.
Carvalho, Marta S.
Udangawa, Ranodhi N.
Moura, Carla
Cabral, Joaquim M. S.
Silva, Cláudia L. da
Ferreira, Frederico Castelo
Vashishth, Deepak
Linhardt, Robert J.
dc.subject.por.fl_str_mv Additive manufacturing
Bone tissue engineering
Cell-derived extracellular matrix
Mesenchymal stem/stromal cells
Polycaprolactone scaffolds
topic Additive manufacturing
Bone tissue engineering
Cell-derived extracellular matrix
Mesenchymal stem/stromal cells
Polycaprolactone scaffolds
description J.C.S. and M.S.C. are grateful to Fundação para a Ciência e Tecnologia (FCT, Portugal) for financial support through the scholarships SFRH/ BD/105771/2014 and SFRH/BD/52478/2014, respectively. The authors acknowledge financial support from FCT through iBB— Institute for Bioengineering and Biosciences (UID/BIO/04565/2019) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and also through the projects PRECISE (PAC-PRECISELISBOA- 01-0145-FEDER-016394) and Stimuli2BioScaffold (FCT grant PTDC/EME-SIS/32554/2017). This study was also supported by Center for Biotechnology and Interdisciplinary Studies-Rensselaer Polytechnic Institute funds and by the National Institutes of Health (Grant # DK111958).
publishDate 2020
dc.date.none.fl_str_mv 2020-01-09
2020-01-09T00:00:00Z
2023-04-19T10:32:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8412
url http://hdl.handle.net/10400.8/8412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, J. C., Carvalho, M. S., Udangawa, R. N., Moura, C. S., Cabral, J. M. S., L. da Silva, C., Ferreira, F. C., Vashishth, D., & Linhardt, R. J. (2020). Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient-tailored bone tissue engineering approach. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108(5), 2153–2166. https://doi.org/10.1002/jbm.b.34554
1552-4973
10.1002/jbm.b.34554
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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