Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx

Detalhes bibliográficos
Autor(a) principal: Moreira, Helena R.
Data de Publicação: 2022
Outros Autores: Rodrigues, Daniel Barreira, Ribeiro, Sara Freitas, Silva, Lucília Pereira, Morais, Alain da S., Jarnalo, Mariana, Horta, Ricardo, Reis, R. L., Pirraco, Rogério P., Marques, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/81630
Resumo: In vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important in cutaneous wound healing. Thus, we aimed to investigate whether in vitro construct prevascularization with SVF accelerates the healing cascade by fostering early vascularization vis-à-vis SVF seeding prior to implantation. Prevascularized constructs delayed re-epithelization of full-thickness mice wounds compared to both non-prevascularized and control (no SVF) groups. Our results suggest this delay is due to a persistent inflammation as indicated by a significantly lower M2(CD163+)/M1(CD86+) macrophage subtype ratio. Moreover, a slower transition from the inflammatory to the proliferative phase of the healing was confirmed by reduced extracellular matrix deposition and increased presence of thick collagen fibers from early time-points, suggesting the prevalence of fiber crosslinking in relation to neodeposition. Overall, while prevascularization potentiates inflammatory cell influx, which negatively impacts the cutaneous wound healing cascade, an effective wound healing was guaranteed in non-prevascularized SVF cell-containing spongy-like hydrogels confirming that the SVF can have enhanced efficacy.
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spelling Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influxInflammationIntegrin-specific biomaterialsRe-epithelizationRemodelingStromal vascular fractionWound healingScience & TechnologyIn vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important in cutaneous wound healing. Thus, we aimed to investigate whether in vitro construct prevascularization with SVF accelerates the healing cascade by fostering early vascularization vis-à-vis SVF seeding prior to implantation. Prevascularized constructs delayed re-epithelization of full-thickness mice wounds compared to both non-prevascularized and control (no SVF) groups. Our results suggest this delay is due to a persistent inflammation as indicated by a significantly lower M2(CD163+)/M1(CD86+) macrophage subtype ratio. Moreover, a slower transition from the inflammatory to the proliferative phase of the healing was confirmed by reduced extracellular matrix deposition and increased presence of thick collagen fibers from early time-points, suggesting the prevalence of fiber crosslinking in relation to neodeposition. Overall, while prevascularization potentiates inflammatory cell influx, which negatively impacts the cutaneous wound healing cascade, an effective wound healing was guaranteed in non-prevascularized SVF cell-containing spongy-like hydrogels confirming that the SVF can have enhanced efficacy.Authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and the Starting Grant “CapBed” (ERC-2018-STG-805411), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08-5369-FSE-000037 (H.R.M.), and to FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the grants SFRH/BD/119756/2016 (D.B.R.), PhD grant PD/BD/135252/2017 (S.F.R.) and IF/00347/2015 (R.P.P.). Authors would also like to acknowledge BioRender.com as a platform for image creation.ElsevierUniversidade do MinhoMoreira, Helena R.Rodrigues, Daniel BarreiraRibeiro, Sara FreitasSilva, Lucília PereiraMorais, Alain da S.Jarnalo, MarianaHorta, RicardoReis, R. L.Pirraco, Rogério P.Marques, A. P.2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/81630engMoreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jarnalo M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx, Materials Today Bio, Vol. 17, pp. 100496, doi:10.1016/j.mtbio.2022.100496, 20222590-00642590-006410.1016/j.mtbio.2022.100496100496https://doi.org/10.1016/j.mtbio.2022.100496info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:33Zoai:repositorium.sdum.uminho.pt:1822/81630Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:01.084838Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
title Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
spellingShingle Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
Moreira, Helena R.
Inflammation
Integrin-specific biomaterials
Re-epithelization
Remodeling
Stromal vascular fraction
Wound healing
Science & Technology
title_short Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
title_full Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
title_fullStr Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
title_full_unstemmed Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
title_sort Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx
author Moreira, Helena R.
author_facet Moreira, Helena R.
Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
author_role author
author2 Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Moreira, Helena R.
Rodrigues, Daniel Barreira
Ribeiro, Sara Freitas
Silva, Lucília Pereira
Morais, Alain da S.
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Pirraco, Rogério P.
Marques, A. P.
dc.subject.por.fl_str_mv Inflammation
Integrin-specific biomaterials
Re-epithelization
Remodeling
Stromal vascular fraction
Wound healing
Science & Technology
topic Inflammation
Integrin-specific biomaterials
Re-epithelization
Remodeling
Stromal vascular fraction
Wound healing
Science & Technology
description In vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important in cutaneous wound healing. Thus, we aimed to investigate whether in vitro construct prevascularization with SVF accelerates the healing cascade by fostering early vascularization vis-à-vis SVF seeding prior to implantation. Prevascularized constructs delayed re-epithelization of full-thickness mice wounds compared to both non-prevascularized and control (no SVF) groups. Our results suggest this delay is due to a persistent inflammation as indicated by a significantly lower M2(CD163+)/M1(CD86+) macrophage subtype ratio. Moreover, a slower transition from the inflammatory to the proliferative phase of the healing was confirmed by reduced extracellular matrix deposition and increased presence of thick collagen fibers from early time-points, suggesting the prevalence of fiber crosslinking in relation to neodeposition. Overall, while prevascularization potentiates inflammatory cell influx, which negatively impacts the cutaneous wound healing cascade, an effective wound healing was guaranteed in non-prevascularized SVF cell-containing spongy-like hydrogels confirming that the SVF can have enhanced efficacy.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/81630
url https://hdl.handle.net/1822/81630
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moreira H. R., Rodrigues D. B., Freitas-Ribeiro S., da Silva L. P., Morais A., Jarnalo M., Horta R., Reis R. L., Pirraco R. P., Marques A. P. Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx, Materials Today Bio, Vol. 17, pp. 100496, doi:10.1016/j.mtbio.2022.100496, 2022
2590-0064
2590-0064
10.1016/j.mtbio.2022.100496
100496
https://doi.org/10.1016/j.mtbio.2022.100496
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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