Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/13241 https://doi.org/10.1017/S1431927614013099 |
Resumo: | Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes. |
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Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa CulturesAmanita ponderosaprotein-bound polysaccharidesDifferent compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.Cambridge University Press2015-03-11T15:39:15Z2015-03-112014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/13241http://hdl.handle.net/10174/13241https://doi.org/10.1017/S1431927614013099engCátia Salvador, M. Rosário Martins, and A. Teresa Caldeira (2014). Microanalysis Characterization of Bioactive Protein- Bound Polysaccharides Produced by Amanita Ponderosa Cultures. Microscopy and Microanalisys. doi:10.1017/S1431927614013099.cscs@uevora.ptmrm@uevora.ptatc@uevora.pt303Salvador, CMartins, M RCaldeira, A Teresainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:58:32Zoai:dspace.uevora.pt:10174/13241Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:06:40.619367Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
title |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
spellingShingle |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures Salvador, C Amanita ponderosa protein-bound polysaccharides |
title_short |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
title_full |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
title_fullStr |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
title_full_unstemmed |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
title_sort |
Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures |
author |
Salvador, C |
author_facet |
Salvador, C Martins, M R Caldeira, A Teresa |
author_role |
author |
author2 |
Martins, M R Caldeira, A Teresa |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Salvador, C Martins, M R Caldeira, A Teresa |
dc.subject.por.fl_str_mv |
Amanita ponderosa protein-bound polysaccharides |
topic |
Amanita ponderosa protein-bound polysaccharides |
description |
Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01T00:00:00Z 2015-03-11T15:39:15Z 2015-03-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/13241 http://hdl.handle.net/10174/13241 https://doi.org/10.1017/S1431927614013099 |
url |
http://hdl.handle.net/10174/13241 https://doi.org/10.1017/S1431927614013099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cátia Salvador, M. Rosário Martins, and A. Teresa Caldeira (2014). Microanalysis Characterization of Bioactive Protein- Bound Polysaccharides Produced by Amanita Ponderosa Cultures. Microscopy and Microanalisys. doi:10.1017/S1431927614013099. cscs@uevora.pt mrm@uevora.pt atc@uevora.pt 303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136551796473856 |