Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures

Detalhes bibliográficos
Autor(a) principal: Salvador, C
Data de Publicação: 2014
Outros Autores: Martins, M R, Caldeira, A Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/13241
https://doi.org/10.1017/S1431927614013099
Resumo: Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.
id RCAP_e78c71d6bd291665fce569ba80eac284
oai_identifier_str oai:dspace.uevora.pt:10174/13241
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa CulturesAmanita ponderosaprotein-bound polysaccharidesDifferent compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.Cambridge University Press2015-03-11T15:39:15Z2015-03-112014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/13241http://hdl.handle.net/10174/13241https://doi.org/10.1017/S1431927614013099engCátia Salvador, M. Rosário Martins, and A. Teresa Caldeira (2014). Microanalysis Characterization of Bioactive Protein- Bound Polysaccharides Produced by Amanita Ponderosa Cultures. Microscopy and Microanalisys. doi:10.1017/S1431927614013099.cscs@uevora.ptmrm@uevora.ptatc@uevora.pt303Salvador, CMartins, M RCaldeira, A Teresainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:58:32Zoai:dspace.uevora.pt:10174/13241Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:06:40.619367Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
title Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
spellingShingle Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
Salvador, C
Amanita ponderosa
protein-bound polysaccharides
title_short Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
title_full Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
title_fullStr Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
title_full_unstemmed Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
title_sort Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures
author Salvador, C
author_facet Salvador, C
Martins, M R
Caldeira, A Teresa
author_role author
author2 Martins, M R
Caldeira, A Teresa
author2_role author
author
dc.contributor.author.fl_str_mv Salvador, C
Martins, M R
Caldeira, A Teresa
dc.subject.por.fl_str_mv Amanita ponderosa
protein-bound polysaccharides
topic Amanita ponderosa
protein-bound polysaccharides
description Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01T00:00:00Z
2015-03-11T15:39:15Z
2015-03-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/13241
http://hdl.handle.net/10174/13241
https://doi.org/10.1017/S1431927614013099
url http://hdl.handle.net/10174/13241
https://doi.org/10.1017/S1431927614013099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cátia Salvador, M. Rosário Martins, and A. Teresa Caldeira (2014). Microanalysis Characterization of Bioactive Protein- Bound Polysaccharides Produced by Amanita Ponderosa Cultures. Microscopy and Microanalisys. doi:10.1017/S1431927614013099.
cscs@uevora.pt
mrm@uevora.pt
atc@uevora.pt
303
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136551796473856