First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/6738 |
Resumo: | Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compared. Cystozoites obtained from skin tissue of one bull were infectious for g-interferon knockout (GKO) mice by intraperitoneal (ip) inoculation. Tachyzoites were detected in the peritoneal cavity, spleen, liver and lung of the mice 5 days post-infection. The parasite could be maintained in GKO mice by ip inoculation for at least 5 passages. Peritoneal washings containing tachyzoites were obtained from infected mice and used to infect five cell lines (Vero, MARC-145, NA42/13, BHK21, KH-R). The best growth of tachyzoites was observed in BHK21 cells, but replication occurred to a smaller extent also in MARC-145, NA42/13 and KH-R cells. Subsequent comparative analyses revealed that after direct infection of these cell lines with cystozoites derived from bovine skin, the growth was best in NA42/13 cells. Considerable replication was also observed in the BHK21 and KH-R cell lines. Our observations on the growth characteristics of Bb-GER1 partially contrast those for other isolates. The preferential growth in particular cell linesmay be characteristic for particular B. besnoiti isolates. A potential association between growth properties and differences in virulence remains to be established. This is the first in vitro isolation of B. besnoiti from cattle in Germany. |
id |
RCAP_e7a067d404240fffee8f6d981a11ce1b |
---|---|
oai_identifier_str |
oai:dspace.uevora.pt:10174/6738 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in GermanyBesnoitia besnoitiisolationBesnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compared. Cystozoites obtained from skin tissue of one bull were infectious for g-interferon knockout (GKO) mice by intraperitoneal (ip) inoculation. Tachyzoites were detected in the peritoneal cavity, spleen, liver and lung of the mice 5 days post-infection. The parasite could be maintained in GKO mice by ip inoculation for at least 5 passages. Peritoneal washings containing tachyzoites were obtained from infected mice and used to infect five cell lines (Vero, MARC-145, NA42/13, BHK21, KH-R). The best growth of tachyzoites was observed in BHK21 cells, but replication occurred to a smaller extent also in MARC-145, NA42/13 and KH-R cells. Subsequent comparative analyses revealed that after direct infection of these cell lines with cystozoites derived from bovine skin, the growth was best in NA42/13 cells. Considerable replication was also observed in the BHK21 and KH-R cell lines. Our observations on the growth characteristics of Bb-GER1 partially contrast those for other isolates. The preferential growth in particular cell linesmay be characteristic for particular B. besnoiti isolates. A potential association between growth properties and differences in virulence remains to be established. This is the first in vitro isolation of B. besnoiti from cattle in Germany.2012-12-10T10:34:27Z2012-12-102009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/6738http://hdl.handle.net/10174/6738porSchares, G.; Basso, W.; Majzoub, M.; Cortes, H.C.E.; Rostaher, A.; Selmair, J.; Hermanns, W.; Conraths, F.J.; Gollnick, N.S.First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany, Veterinary Parasitology, 163, 4, 315-322, 2009.0304-4017DMVndndndhcec@uevora.ptndndndndnd384Schares, G.Basso, W.Majzoub, M.Cortes, H.C.E.Rostaher, A.Selmair, J.Hermanns, W.Conraths, F.J.Gollnick, N.S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:05Zoai:dspace.uevora.pt:10174/6738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:07.186026Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
title |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
spellingShingle |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany Schares, G. Besnoitia besnoiti isolation |
title_short |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
title_full |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
title_fullStr |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
title_full_unstemmed |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
title_sort |
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany |
author |
Schares, G. |
author_facet |
Schares, G. Basso, W. Majzoub, M. Cortes, H.C.E. Rostaher, A. Selmair, J. Hermanns, W. Conraths, F.J. Gollnick, N.S. |
author_role |
author |
author2 |
Basso, W. Majzoub, M. Cortes, H.C.E. Rostaher, A. Selmair, J. Hermanns, W. Conraths, F.J. Gollnick, N.S. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Schares, G. Basso, W. Majzoub, M. Cortes, H.C.E. Rostaher, A. Selmair, J. Hermanns, W. Conraths, F.J. Gollnick, N.S. |
dc.subject.por.fl_str_mv |
Besnoitia besnoiti isolation |
topic |
Besnoitia besnoiti isolation |
description |
Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compared. Cystozoites obtained from skin tissue of one bull were infectious for g-interferon knockout (GKO) mice by intraperitoneal (ip) inoculation. Tachyzoites were detected in the peritoneal cavity, spleen, liver and lung of the mice 5 days post-infection. The parasite could be maintained in GKO mice by ip inoculation for at least 5 passages. Peritoneal washings containing tachyzoites were obtained from infected mice and used to infect five cell lines (Vero, MARC-145, NA42/13, BHK21, KH-R). The best growth of tachyzoites was observed in BHK21 cells, but replication occurred to a smaller extent also in MARC-145, NA42/13 and KH-R cells. Subsequent comparative analyses revealed that after direct infection of these cell lines with cystozoites derived from bovine skin, the growth was best in NA42/13 cells. Considerable replication was also observed in the BHK21 and KH-R cell lines. Our observations on the growth characteristics of Bb-GER1 partially contrast those for other isolates. The preferential growth in particular cell linesmay be characteristic for particular B. besnoiti isolates. A potential association between growth properties and differences in virulence remains to be established. This is the first in vitro isolation of B. besnoiti from cattle in Germany. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01T00:00:00Z 2012-12-10T10:34:27Z 2012-12-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/6738 http://hdl.handle.net/10174/6738 |
url |
http://hdl.handle.net/10174/6738 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Schares, G.; Basso, W.; Majzoub, M.; Cortes, H.C.E.; Rostaher, A.; Selmair, J.; Hermanns, W.; Conraths, F.J.; Gollnick, N.S.First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany, Veterinary Parasitology, 163, 4, 315-322, 2009. 0304-4017 DMV nd nd nd hcec@uevora.pt nd nd nd nd nd 384 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136473809682432 |