Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates

Detalhes bibliográficos
Autor(a) principal: Santos, Raquel
Data de Publicação: 2016
Outros Autores: Gomes, Diana, Macedo, Hermes, Barros, Diogo, Tibério, Catarina, Veiga, Ana Salomé, Tavares, Luís, Castanho, Miguel A. R. B., Oliveira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51455
Resumo: © 2016 The Authors
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spelling Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolatesAntimicrobial peptideDiabetic foot ulcerGuar gumNisinStaphylococcus aureus© 2016 The AuthorsDiabetic patients frequently develop diabetic foot ulcers (DFUs), particularly those patients vulnerable to Staphylococcus aureus opportunistic infections. It is urgent to find new treatments for bacterial infections. The antimicrobial peptide (AMP) nisin is a potential candidate, mainly due to its broad spectrum of action against pathogens. Considering that AMP can be degraded or inactivated before reaching its target at therapeutic concentrations, it is mandatory to establish effective AMP delivery systems, with the natural polysaccharide guar gum being one of the most promising. We analysed the antimicrobial potential of nisin against 23 S. aureus DFU biofilm-producing isolates. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were determined for nisin diluted in HCl and incorporated in guar gum gel. Statistical analysis was performed using the Wilcoxon matched-pair test. Nisin was effective against all isolates, including some multidrug-resistant clinical isolates, independent of whether it is incorporated in guar gum. While differences among MIC, MBC and MBIC values were observed for HCl- and guar gum- nisin, no significant differences were found between MBEC values. Inhibitory activity of both systems seems to differ only twofold, which does not compromise guar gum gel efficiency as a delivery system. Our results highlight the potential of nisin as a substitute for or complementary therapy to current antibiotics used for treating DFU infections, which is extremely relevant considering the increase in multidrug-resistant bacteria dissemination. The guar gum gel represents an alternative, practical and safe delivery system for AMPs, allowing the development of novel topical therapies as treatments for bacterial skin infections.The authors would like to acknowledge the Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa [Project UID/CVT/00276/2013, funded by Fundação para a Ciência e Tecnologia (FCT), Portugal]. This study was also conducted with the financial support of the project PTDC/SAU-MIC/122816/2010: ‘Biofilms in diabetic foot: microbial virulence characterization and cross-talk of major isolates’, funded by the FCT, Portugal. Raquel Santos, Diogo Barros and Ana Salomé Veiga acknowledge the FCT, Portugal, respectively, for two PhD fellowships (SFRH/BD/100571/2014 and PD/BD/113457/2015) and fellowship IF/00803/2012 under the FCT Investigator Programme.Microbiology SocietyRepositório da Universidade de LisboaSantos, RaquelGomes, DianaMacedo, HermesBarros, DiogoTibério, CatarinaVeiga, Ana SaloméTavares, LuísCastanho, Miguel A. R. B.Oliveira, Manuela2022-02-22T16:13:12Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51455engJournal of Medical Microbiology (2016), 65, 1092–10990022-261510.1099/jmm.0.0003291473-5644info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:09Zoai:repositorio.ul.pt:10451/51455Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:44.338842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
title Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
spellingShingle Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
Santos, Raquel
Antimicrobial peptide
Diabetic foot ulcer
Guar gum
Nisin
Staphylococcus aureus
title_short Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
title_full Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
title_fullStr Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
title_full_unstemmed Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
title_sort Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates
author Santos, Raquel
author_facet Santos, Raquel
Gomes, Diana
Macedo, Hermes
Barros, Diogo
Tibério, Catarina
Veiga, Ana Salomé
Tavares, Luís
Castanho, Miguel A. R. B.
Oliveira, Manuela
author_role author
author2 Gomes, Diana
Macedo, Hermes
Barros, Diogo
Tibério, Catarina
Veiga, Ana Salomé
Tavares, Luís
Castanho, Miguel A. R. B.
Oliveira, Manuela
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Raquel
Gomes, Diana
Macedo, Hermes
Barros, Diogo
Tibério, Catarina
Veiga, Ana Salomé
Tavares, Luís
Castanho, Miguel A. R. B.
Oliveira, Manuela
dc.subject.por.fl_str_mv Antimicrobial peptide
Diabetic foot ulcer
Guar gum
Nisin
Staphylococcus aureus
topic Antimicrobial peptide
Diabetic foot ulcer
Guar gum
Nisin
Staphylococcus aureus
description © 2016 The Authors
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2022-02-22T16:13:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51455
url http://hdl.handle.net/10451/51455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Medical Microbiology (2016), 65, 1092–1099
0022-2615
10.1099/jmm.0.000329
1473-5644
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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