Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31493 |
Resumo: | Aims To characterise and explore the potential in extracellular biosynthesis of silver nanoparticles (AgNPs) by Penicillium chrysogenum and Aspergillus oryzae and to investigate the antifungal effect of chemically vs. biologically synthesised AgNPs comparing with conventional antifungal drugs against Trichophyton rubrum. Methods and Results Chemically synthesised AgNPs (Chem-AgNPs) coated with polyvinylpyrrolidone (PVP) were synthesised by chemical reduction method with glucose in PVP aqueous solution. Biologically synthesised AgNPs (Bio-AgNPs) were produced from the extracellular cell-free filtrate of Penicillium chrysogenum MUM 03.22 and Aspergillus oryzae MUM 97.19. Among the commercial antifungal drugs terbinafine exhibited the lower MIC range values of 0.063 to 0.25 μg ml-1 for the clinical strains. Chem-AgNPs exhibited antifungal activity against all T. rubrum strains. Bio-AgNPs produced by the fungal cell-free filtrate of P. chrysogenum showed an antifungal activity higher than fluconazole but less than terbinafine, itraconazole and Chem-AgNPs. Conclusion The synthesis parameters in future works should be carefully studied to take full advantage of all the potential of filamentous fungi in the synthesis of AgNPs. Significance and Impact of the study: Bio-AgNPs could be used as antifungal agents, namely against dermatophytes |
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Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrumAntimicrobialsFungiInfectionMycologyDermatophytesScience & TechnologyAims To characterise and explore the potential in extracellular biosynthesis of silver nanoparticles (AgNPs) by Penicillium chrysogenum and Aspergillus oryzae and to investigate the antifungal effect of chemically vs. biologically synthesised AgNPs comparing with conventional antifungal drugs against Trichophyton rubrum. Methods and Results Chemically synthesised AgNPs (Chem-AgNPs) coated with polyvinylpyrrolidone (PVP) were synthesised by chemical reduction method with glucose in PVP aqueous solution. Biologically synthesised AgNPs (Bio-AgNPs) were produced from the extracellular cell-free filtrate of Penicillium chrysogenum MUM 03.22 and Aspergillus oryzae MUM 97.19. Among the commercial antifungal drugs terbinafine exhibited the lower MIC range values of 0.063 to 0.25 μg ml-1 for the clinical strains. Chem-AgNPs exhibited antifungal activity against all T. rubrum strains. Bio-AgNPs produced by the fungal cell-free filtrate of P. chrysogenum showed an antifungal activity higher than fluconazole but less than terbinafine, itraconazole and Chem-AgNPs. Conclusion The synthesis parameters in future works should be carefully studied to take full advantage of all the potential of filamentous fungi in the synthesis of AgNPs. Significance and Impact of the study: Bio-AgNPs could be used as antifungal agents, namely against dermatophytesThe authors thank Pedro Martins (Physics Department of University of Minho) for help in XRD analysis. The authors thank to SDBSWeb: http://sdbs.db.aist.go.jp (Japanese National Institute of Advanced Industrial Science and Technology). The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013. Nicolina Dias acknowledges the project 'Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB', RECI/BBB-EBI/0179/2012.Blackwell Publishing Inc.Universidade do MinhoPereira, LeonelDias, NicolinaCarvalho, JulianaFernandes, SaraSantos, CledirLima, Nelson2014-01-012014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31493engPereira, Leonel; Dias, Nicolina; Carvalho, J.; Fernandes, Sara; Santos, C.; Lima, Nelson, Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum. Journal of Applied Microbiology, 117(6), 1601-1613, 20141364-50721365-267210.1111/jam.1265225234047info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:04Zoai:repositorium.sdum.uminho.pt:1822/31493Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:27.900840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
title |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
spellingShingle |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum Pereira, Leonel Antimicrobials Fungi Infection Mycology Dermatophytes Science & Technology |
title_short |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
title_full |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
title_fullStr |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
title_full_unstemmed |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
title_sort |
Synthesis, characterisation and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum |
author |
Pereira, Leonel |
author_facet |
Pereira, Leonel Dias, Nicolina Carvalho, Juliana Fernandes, Sara Santos, Cledir Lima, Nelson |
author_role |
author |
author2 |
Dias, Nicolina Carvalho, Juliana Fernandes, Sara Santos, Cledir Lima, Nelson |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Leonel Dias, Nicolina Carvalho, Juliana Fernandes, Sara Santos, Cledir Lima, Nelson |
dc.subject.por.fl_str_mv |
Antimicrobials Fungi Infection Mycology Dermatophytes Science & Technology |
topic |
Antimicrobials Fungi Infection Mycology Dermatophytes Science & Technology |
description |
Aims To characterise and explore the potential in extracellular biosynthesis of silver nanoparticles (AgNPs) by Penicillium chrysogenum and Aspergillus oryzae and to investigate the antifungal effect of chemically vs. biologically synthesised AgNPs comparing with conventional antifungal drugs against Trichophyton rubrum. Methods and Results Chemically synthesised AgNPs (Chem-AgNPs) coated with polyvinylpyrrolidone (PVP) were synthesised by chemical reduction method with glucose in PVP aqueous solution. Biologically synthesised AgNPs (Bio-AgNPs) were produced from the extracellular cell-free filtrate of Penicillium chrysogenum MUM 03.22 and Aspergillus oryzae MUM 97.19. Among the commercial antifungal drugs terbinafine exhibited the lower MIC range values of 0.063 to 0.25 μg ml-1 for the clinical strains. Chem-AgNPs exhibited antifungal activity against all T. rubrum strains. Bio-AgNPs produced by the fungal cell-free filtrate of P. chrysogenum showed an antifungal activity higher than fluconazole but less than terbinafine, itraconazole and Chem-AgNPs. Conclusion The synthesis parameters in future works should be carefully studied to take full advantage of all the potential of filamentous fungi in the synthesis of AgNPs. Significance and Impact of the study: Bio-AgNPs could be used as antifungal agents, namely against dermatophytes |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31493 |
url |
http://hdl.handle.net/1822/31493 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, Leonel; Dias, Nicolina; Carvalho, J.; Fernandes, Sara; Santos, C.; Lima, Nelson, Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum. Journal of Applied Microbiology, 117(6), 1601-1613, 2014 1364-5072 1365-2672 10.1111/jam.12652 25234047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing Inc. |
publisher.none.fl_str_mv |
Blackwell Publishing Inc. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133048190533632 |