Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/39736 |
Resumo: | Demineralized and disinfected waters may have very low microbial loads, requiring that large volumes of water are filtered to recover enough biomass for further analysis. Extended filtration periods, often interrupted by clogging, are a major limiting factor to concentrate samples' microbiota for further examination, besides hindering the work pace. In this study, we investigated the performance of three types of filtering membranes - polycarbonate (PC), cellulose nitrate (CN), and polyethersulfone (PES) with 0.22 μm pore size for culture-independent microbiological analysis (quantitative PCR of seven housekeeping and integrase genes) of tap water, recirculating tap water in a bottle washing loop, and of demineralized water. Compared to PC membranes, CN or PES required lower filtration periods, although had slightly lower DNA extraction yields. However, genes abundance per volume of water was, in general, not significantly different. The exception was observed for bottle washing water in which PC membranes supported significantly higher quantification values than PES membranes. These differences were lower than ∼0.5 log-units and did not hamper the distinction of the types of water based on genes profile. Also, the type of membrane did not significantly affect the profile of the bacterial community determined for tap and demineralized water. A major conclusion is that CN membranes, cheaper, allowing shorter filtration periods, and producing results that are not significantly different from those obtained with PC or PES, can be a good alternative to analyze waters with low biomass loads. |
id |
RCAP_e8822f267a30e968cfe1533a08904159 |
---|---|
oai_identifier_str |
oai:repositorio.ucp.pt:10400.14/39736 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean watersCellulose nitrate (CN)Clean watersFilter membranesMicrobiological analysisPolycarbonate (PC)Polyethersulfone (PES)Demineralized and disinfected waters may have very low microbial loads, requiring that large volumes of water are filtered to recover enough biomass for further analysis. Extended filtration periods, often interrupted by clogging, are a major limiting factor to concentrate samples' microbiota for further examination, besides hindering the work pace. In this study, we investigated the performance of three types of filtering membranes - polycarbonate (PC), cellulose nitrate (CN), and polyethersulfone (PES) with 0.22 μm pore size for culture-independent microbiological analysis (quantitative PCR of seven housekeeping and integrase genes) of tap water, recirculating tap water in a bottle washing loop, and of demineralized water. Compared to PC membranes, CN or PES required lower filtration periods, although had slightly lower DNA extraction yields. However, genes abundance per volume of water was, in general, not significantly different. The exception was observed for bottle washing water in which PC membranes supported significantly higher quantification values than PES membranes. These differences were lower than ∼0.5 log-units and did not hamper the distinction of the types of water based on genes profile. Also, the type of membrane did not significantly affect the profile of the bacterial community determined for tap and demineralized water. A major conclusion is that CN membranes, cheaper, allowing shorter filtration periods, and producing results that are not significantly different from those obtained with PC or PES, can be a good alternative to analyze waters with low biomass loads.Veritati - Repositório Institucional da Universidade Católica PortuguesaAbreu-Silva, JoanaRibeirinho-Soares, SaraOliveira-Inocêncio, InêsPedrosa, MartaSilva, Adrián M. T.Nunes, Olga C.Manaia, Célia M.2023-01-04T18:09:30Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39736eng2213-343710.1016/j.jece.2022.10913285144426112000901816800002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:45:17Zoai:repositorio.ucp.pt:10400.14/39736Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:32:31.712307Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
title |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
spellingShingle |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters Abreu-Silva, Joana Cellulose nitrate (CN) Clean waters Filter membranes Microbiological analysis Polycarbonate (PC) Polyethersulfone (PES) |
title_short |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
title_full |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
title_fullStr |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
title_full_unstemmed |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
title_sort |
Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membranes for culture-independent microbiota analysis of clean waters |
author |
Abreu-Silva, Joana |
author_facet |
Abreu-Silva, Joana Ribeirinho-Soares, Sara Oliveira-Inocêncio, Inês Pedrosa, Marta Silva, Adrián M. T. Nunes, Olga C. Manaia, Célia M. |
author_role |
author |
author2 |
Ribeirinho-Soares, Sara Oliveira-Inocêncio, Inês Pedrosa, Marta Silva, Adrián M. T. Nunes, Olga C. Manaia, Célia M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Abreu-Silva, Joana Ribeirinho-Soares, Sara Oliveira-Inocêncio, Inês Pedrosa, Marta Silva, Adrián M. T. Nunes, Olga C. Manaia, Célia M. |
dc.subject.por.fl_str_mv |
Cellulose nitrate (CN) Clean waters Filter membranes Microbiological analysis Polycarbonate (PC) Polyethersulfone (PES) |
topic |
Cellulose nitrate (CN) Clean waters Filter membranes Microbiological analysis Polycarbonate (PC) Polyethersulfone (PES) |
description |
Demineralized and disinfected waters may have very low microbial loads, requiring that large volumes of water are filtered to recover enough biomass for further analysis. Extended filtration periods, often interrupted by clogging, are a major limiting factor to concentrate samples' microbiota for further examination, besides hindering the work pace. In this study, we investigated the performance of three types of filtering membranes - polycarbonate (PC), cellulose nitrate (CN), and polyethersulfone (PES) with 0.22 μm pore size for culture-independent microbiological analysis (quantitative PCR of seven housekeeping and integrase genes) of tap water, recirculating tap water in a bottle washing loop, and of demineralized water. Compared to PC membranes, CN or PES required lower filtration periods, although had slightly lower DNA extraction yields. However, genes abundance per volume of water was, in general, not significantly different. The exception was observed for bottle washing water in which PC membranes supported significantly higher quantification values than PES membranes. These differences were lower than ∼0.5 log-units and did not hamper the distinction of the types of water based on genes profile. Also, the type of membrane did not significantly affect the profile of the bacterial community determined for tap and demineralized water. A major conclusion is that CN membranes, cheaper, allowing shorter filtration periods, and producing results that are not significantly different from those obtained with PC or PES, can be a good alternative to analyze waters with low biomass loads. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-04T18:09:30Z 2023-02 2023-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/39736 |
url |
http://hdl.handle.net/10400.14/39736 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2213-3437 10.1016/j.jece.2022.109132 85144426112 000901816800002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132049259364352 |