Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases

Detalhes bibliográficos
Autor(a) principal: Costa, Júlia
Data de Publicação: 2020
Outros Autores: Pronto Laborinho, Ana Catarina, Pinto, Susana, Gromicho, Marta, Bonucci, Sara, Tranfield, Erin, Correia, Catarina, Alexandre, Bruno M., Carvalho, Mamede
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49899
Resumo: © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases© The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Galectin-3 binding protein (LGALS3BP or 90 K) is a secreted glycoprotein found in human body fluids. Deregulated levels were observed in cancer and infection and its study in neurological diseases is more recent. Here, we have investigated 90 K from human cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS, n = 35) and other neurological diseases (n = 23). CSF was fractionated by ultrafiltration/size-exclusion chromatography (SEC) and eluted fractions were analysed by complementary techniques including immunoblotting, electron microscopy and nano-liquid chromatography-tandem mass spectrometry. A fraction of 90 K appeared as nanoparticles of irregular shape with heterogeneous dimensions of 15-60 nm that co-eluted with extracellular vesicles in SEC. Median levels of 90 K quantified by ELISA were not different between ALS patients (215.8 ng/ml) and controls (213.3 ng/ml) in contrast with the benchmark biomarker for ALS phosphoneurofilament heavy chain (1750 and 345 pg/ml, respectively). A multiregression model supported age is the only independent predictor of 90 K level in both groups (p < 0.05). Significant correlation was found between 90 K levels and age for the ALS group (r = 0.366, p = 0.031) and for all subjects (r = 0.392, p = 0.003). In conclusion, this study unveils the presence of 90 K-containing nanoparticles in human CSF and opens novel perspectives to further investigate 90 K as potential aging marker.This work was supported by Euronanomed 2 ERA-NET project GlioEx (ENMed/0001/2013), Fundação para a Ciência e a Tecnologia (FCT), Portugal; iNOVA4Health Research Unit (LISBOA-01–0145-FEDER-007344), which is cofunded by FCT/Ministério da Ciência e do Ensino Superior, through national funds; and by FEDER under the PT2020 Partnership Agreement.Springer NatureRepositório da Universidade de LisboaCosta, JúliaPronto Laborinho, Ana CatarinaPinto, SusanaGromicho, MartaBonucci, SaraTranfield, ErinCorreia, CatarinaAlexandre, Bruno M.Carvalho, Mamede2021-10-15T13:10:47Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49899engSci Rep. 2020 Mar 27;10(1):564910.1038/s41598-020-62592-w2045-2322info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:51Zoai:repositorio.ul.pt:10451/49899Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:25.488525Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
title Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
spellingShingle Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
Costa, Júlia
title_short Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
title_full Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
title_fullStr Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
title_full_unstemmed Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
title_sort Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
author Costa, Júlia
author_facet Costa, Júlia
Pronto Laborinho, Ana Catarina
Pinto, Susana
Gromicho, Marta
Bonucci, Sara
Tranfield, Erin
Correia, Catarina
Alexandre, Bruno M.
Carvalho, Mamede
author_role author
author2 Pronto Laborinho, Ana Catarina
Pinto, Susana
Gromicho, Marta
Bonucci, Sara
Tranfield, Erin
Correia, Catarina
Alexandre, Bruno M.
Carvalho, Mamede
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Costa, Júlia
Pronto Laborinho, Ana Catarina
Pinto, Susana
Gromicho, Marta
Bonucci, Sara
Tranfield, Erin
Correia, Catarina
Alexandre, Bruno M.
Carvalho, Mamede
description © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-10-15T13:10:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49899
url http://hdl.handle.net/10451/49899
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sci Rep. 2020 Mar 27;10(1):5649
10.1038/s41598-020-62592-w
2045-2322
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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