Active APPL1 sequestration by Plasmodium favors liver-stage development

Detalhes bibliográficos
Autor(a) principal: Lahree, Aparajita
Data de Publicação: 2022
Outros Autores: Baptista, Sara de Jesus Santos, Marques, Sofia, Perschin, Veronika, Zuzarte-Luis, Vanessa, Goel, Manisha, Choudhary, Hadi Hasan, Mishra, Satish, Stigloher, Christian, Zerial, Marino, Sundaramurthy, Varadharajan, Mota, Maria M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/53296
Resumo: © 2022 The Authors. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/).
id RCAP_e8d1ef971ce836033988d81e23de308b
oai_identifier_str oai:repositorio.ul.pt:10451/53296
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Active APPL1 sequestration by Plasmodium favors liver-stage developmentAPPL1CPMicrobiologyPbRab5bPlasmodiumEndosomeHepatocytesLiver-stageMalaria© 2022 The Authors. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/).Intracellular pathogens manipulate host cells to survive and thrive. Cellular sensing and signaling pathways are among the key host machineries deregulated to favor infection. In this study, we show that liver-stage Plasmodium parasites compete with the host to sequester a host endosomal-adaptor protein (APPL1) known to regulate signaling in response to endocytosis. The enrichment of APPL1 at the parasitophorous vacuole membrane (PVM) involves an atypical Plasmodium Rab5 isoform (Rab5b). Depletion of host APPL1 alters neither the infection nor parasite development; however, upon overexpression of a GTPase-deficient host Rab5 mutant (hRab5_Q79L), the parasites are smaller and their PVM is stripped of APPL1. Infection with the GTPase-deficient Plasmodium berghei Rab5b mutant (PbRab5b_Q91L) in this case rescues the PVM APPL1 signal and parasite size. In summary, we observe a robust correlation between the level of APPL1 retention at the PVM and parasite size during exoerythrocytic development.This work was supported by grants from the la Caixa Banking Foundation and Fundação para a Ciência e a Tecnologia (HR17-00264 and PTDC/SAU-PAR/30751/2017 respectively, both to M.M.M.). A.L and S.S.B were sponsored by Fundação para a Ciência e a Tecnologia fellowships (PD/BD/114036/2015, SFRH/BD/114464/2016, respectively). V.S acknowledges funding from the Science and Engineering Research Board (SERB) (EMR/2016/006810), Department of Science and Technology (DST), Government of India. V.P. and C.S. were supported by GRK2581 (P6) SPHINGOINF of the Growing Spine Foundation (DFG).ElsevierRepositório da Universidade de LisboaLahree, AparajitaBaptista, Sara de Jesus SantosMarques, SofiaPerschin, VeronikaZuzarte-Luis, VanessaGoel, ManishaChoudhary, Hadi HasanMishra, SatishStigloher, ChristianZerial, MarinoSundaramurthy, VaradharajanMota, Maria M.2022-06-06T15:38:55Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53296engCell Rep. 2022 May 31;39(9):1108862211-124710.1016/j.celrep.2022.110886info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:59:00Zoai:repositorio.ul.pt:10451/53296Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:16.342730Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Active APPL1 sequestration by Plasmodium favors liver-stage development
title Active APPL1 sequestration by Plasmodium favors liver-stage development
spellingShingle Active APPL1 sequestration by Plasmodium favors liver-stage development
Lahree, Aparajita
APPL1
CP
Microbiology
PbRab5b
Plasmodium
Endosome
Hepatocytes
Liver-stage
Malaria
title_short Active APPL1 sequestration by Plasmodium favors liver-stage development
title_full Active APPL1 sequestration by Plasmodium favors liver-stage development
title_fullStr Active APPL1 sequestration by Plasmodium favors liver-stage development
title_full_unstemmed Active APPL1 sequestration by Plasmodium favors liver-stage development
title_sort Active APPL1 sequestration by Plasmodium favors liver-stage development
author Lahree, Aparajita
author_facet Lahree, Aparajita
Baptista, Sara de Jesus Santos
Marques, Sofia
Perschin, Veronika
Zuzarte-Luis, Vanessa
Goel, Manisha
Choudhary, Hadi Hasan
Mishra, Satish
Stigloher, Christian
Zerial, Marino
Sundaramurthy, Varadharajan
Mota, Maria M.
author_role author
author2 Baptista, Sara de Jesus Santos
Marques, Sofia
Perschin, Veronika
Zuzarte-Luis, Vanessa
Goel, Manisha
Choudhary, Hadi Hasan
Mishra, Satish
Stigloher, Christian
Zerial, Marino
Sundaramurthy, Varadharajan
Mota, Maria M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lahree, Aparajita
Baptista, Sara de Jesus Santos
Marques, Sofia
Perschin, Veronika
Zuzarte-Luis, Vanessa
Goel, Manisha
Choudhary, Hadi Hasan
Mishra, Satish
Stigloher, Christian
Zerial, Marino
Sundaramurthy, Varadharajan
Mota, Maria M.
dc.subject.por.fl_str_mv APPL1
CP
Microbiology
PbRab5b
Plasmodium
Endosome
Hepatocytes
Liver-stage
Malaria
topic APPL1
CP
Microbiology
PbRab5b
Plasmodium
Endosome
Hepatocytes
Liver-stage
Malaria
description © 2022 The Authors. This is an open access article under the CC BY-NC-ND license http://creativecommons.org/licenses/by-nc-nd/4.0/).
publishDate 2022
dc.date.none.fl_str_mv 2022-06-06T15:38:55Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/53296
url http://hdl.handle.net/10451/53296
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell Rep. 2022 May 31;39(9):110886
2211-1247
10.1016/j.celrep.2022.110886
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134593127809024

Registros relacionados