Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue?
Main Author: | |
---|---|
Publication Date: | 2020 |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.18/7620 |
Summary: | The prevalence and diversity of Staphylococcus species from wild European rabbits (Oryctolagus cuniculus) in the Azores were investigated, and the antibiotic resistance phenotype and genotype of the isolates were determined. Nasal samples from 77 wild European rabbits from São Jorge and São Miguel islands in Azores were examined. Antibiotic susceptibility of the isolates was determined using the Kirby-Bauer disk diffusion method, and the presence of antimicrobial resistance genes and virulence factors was determined by PCR. The genetic lineages of S. aureus isolates were characterized by spa typing and multilocus sequence typing. A total of 49 staphylococci were obtained from 35 of the 77 wild rabbits. Both coagulase-positive (8.2%) and coagulase-negative (91.8%) staphylococci were detected: 4 S. aureus, 17 S. fleurettii, 13 S. sciuri, 7 S. xylosus, 4 S. epidermidis, and 1 each of S. simulans, S. saprophyticus, S. succinus, and S. equorum. The four S. aureus isolates showed methicillin susceptibility and were characterized as spa type t272/CC121, Panton-Valentine leukocidin negative, and hlB positive. Most of the coagulase-negative staphylococci showed resistance to fusidic acid and beta-lactams, and multidrug resistance was identified especially among S. epidermidis isolates. The mecA gene was detected in 20 isolates of the species S. fleurettii and S. epidermidis, associated with the blaZ gene in one S. epidermidis isolate. Five antimicrobial resistance genes were detected in one S. epidermidis isolate (mecA,dfrA,dfrG,aac6'-aph2'', and ant4). Our results highlight that wild rabbits are reservoirs or "temporary hosts" of Staphylococcus species with zoonotic potential, some of them carrying relevant antimicrobial resistances. |
id |
RCAP_e8d286c82b13f82434522ca7acbc273c |
---|---|
oai_identifier_str |
oai:repositorio.insa.pt:10400.18/7620 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue?AnimalsAnti-Bacterial AgentsAzoresMethicillinMicrobial Sensitivity TestsRabbitsStaphylococcus aureusStaphylococcal InfectionsStaphylococcusResistência aos AntimicrobianosThe prevalence and diversity of Staphylococcus species from wild European rabbits (Oryctolagus cuniculus) in the Azores were investigated, and the antibiotic resistance phenotype and genotype of the isolates were determined. Nasal samples from 77 wild European rabbits from São Jorge and São Miguel islands in Azores were examined. Antibiotic susceptibility of the isolates was determined using the Kirby-Bauer disk diffusion method, and the presence of antimicrobial resistance genes and virulence factors was determined by PCR. The genetic lineages of S. aureus isolates were characterized by spa typing and multilocus sequence typing. A total of 49 staphylococci were obtained from 35 of the 77 wild rabbits. Both coagulase-positive (8.2%) and coagulase-negative (91.8%) staphylococci were detected: 4 S. aureus, 17 S. fleurettii, 13 S. sciuri, 7 S. xylosus, 4 S. epidermidis, and 1 each of S. simulans, S. saprophyticus, S. succinus, and S. equorum. The four S. aureus isolates showed methicillin susceptibility and were characterized as spa type t272/CC121, Panton-Valentine leukocidin negative, and hlB positive. Most of the coagulase-negative staphylococci showed resistance to fusidic acid and beta-lactams, and multidrug resistance was identified especially among S. epidermidis isolates. The mecA gene was detected in 20 isolates of the species S. fleurettii and S. epidermidis, associated with the blaZ gene in one S. epidermidis isolate. Five antimicrobial resistance genes were detected in one S. epidermidis isolate (mecA,dfrA,dfrG,aac6'-aph2'', and ant4). Our results highlight that wild rabbits are reservoirs or "temporary hosts" of Staphylococcus species with zoonotic potential, some of them carrying relevant antimicrobial resistances.Highlights: Staphylococci are frequently detected in nasal samples of wild rabbits; Main Staphylococcus spp. (of 9 detected): fleurettii, sciuri, xylosus, epidermidis, and aureus; All S. aureus were MSSA and typed as t272/CC121; The mecA gene was detected in 44% of CoNS; all were of S. epidermidis or S. fleurettii species; Fusidic acid resistance is detected in most of CoNS, and multidrug resistance in S. epidermidis.This work was funded by the R&D Project CAREBIO2— Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This research was supported by funding from the Research Group SEGURALI. Margarida Sousa and Vanessa Silva have their Ph.D. fellowships granted by FCT (Fundação para a Ciência e a Tecnologia, Portugal) with the references SFRH/BD/87302/2012 and SFRH/BD/137947/2018, respectively. Nuno Silva was supported by “Programa Ciência 2008” cofinanced by POPH/QREN Type 4.2: Employment Promotion Scientific subsidized by the European Social Fund and National Funds of the Ministry of Science and Technology for Higher Education (MCTES).International Association for Food ProtectionRepositório Científico do Instituto Nacional de SaúdeSousa, M.Silva, V.Silva, A.Silva, N.Ribeiro, J.Tejedor-Junco, M.T.Capita, R.Chenouf, N.S.Alonso-Calleja, C.Rodrigues, T.M.Leitão, M.Gonçalves, D.Caniça, M.Torres, C.Igrejas, G.Poeta, P.2021-03-31T17:08:11Z2020-07-012020-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7620engJ Food Prot. 2020 Jul 1;83(7):1110-1114. doi: 10.4315/0362-028X.JFP-19-423.0362-028X10.4315/0362-028X.JFP-19-423info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:06Zoai:repositorio.insa.pt:10400.18/7620Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:15.029458Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
title |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
spellingShingle |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? Sousa, M. Animals Anti-Bacterial Agents Azores Methicillin Microbial Sensitivity Tests Rabbits Staphylococcus aureus Staphylococcal Infections Staphylococcus Resistência aos Antimicrobianos |
title_short |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
title_full |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
title_fullStr |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
title_full_unstemmed |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
title_sort |
Staphylococci among Wild European Rabbits from the Azores: A Potential Zoonotic Issue? |
author |
Sousa, M. |
author_facet |
Sousa, M. Silva, V. Silva, A. Silva, N. Ribeiro, J. Tejedor-Junco, M.T. Capita, R. Chenouf, N.S. Alonso-Calleja, C. Rodrigues, T.M. Leitão, M. Gonçalves, D. Caniça, M. Torres, C. Igrejas, G. Poeta, P. |
author_role |
author |
author2 |
Silva, V. Silva, A. Silva, N. Ribeiro, J. Tejedor-Junco, M.T. Capita, R. Chenouf, N.S. Alonso-Calleja, C. Rodrigues, T.M. Leitão, M. Gonçalves, D. Caniça, M. Torres, C. Igrejas, G. Poeta, P. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Sousa, M. Silva, V. Silva, A. Silva, N. Ribeiro, J. Tejedor-Junco, M.T. Capita, R. Chenouf, N.S. Alonso-Calleja, C. Rodrigues, T.M. Leitão, M. Gonçalves, D. Caniça, M. Torres, C. Igrejas, G. Poeta, P. |
dc.subject.por.fl_str_mv |
Animals Anti-Bacterial Agents Azores Methicillin Microbial Sensitivity Tests Rabbits Staphylococcus aureus Staphylococcal Infections Staphylococcus Resistência aos Antimicrobianos |
topic |
Animals Anti-Bacterial Agents Azores Methicillin Microbial Sensitivity Tests Rabbits Staphylococcus aureus Staphylococcal Infections Staphylococcus Resistência aos Antimicrobianos |
description |
The prevalence and diversity of Staphylococcus species from wild European rabbits (Oryctolagus cuniculus) in the Azores were investigated, and the antibiotic resistance phenotype and genotype of the isolates were determined. Nasal samples from 77 wild European rabbits from São Jorge and São Miguel islands in Azores were examined. Antibiotic susceptibility of the isolates was determined using the Kirby-Bauer disk diffusion method, and the presence of antimicrobial resistance genes and virulence factors was determined by PCR. The genetic lineages of S. aureus isolates were characterized by spa typing and multilocus sequence typing. A total of 49 staphylococci were obtained from 35 of the 77 wild rabbits. Both coagulase-positive (8.2%) and coagulase-negative (91.8%) staphylococci were detected: 4 S. aureus, 17 S. fleurettii, 13 S. sciuri, 7 S. xylosus, 4 S. epidermidis, and 1 each of S. simulans, S. saprophyticus, S. succinus, and S. equorum. The four S. aureus isolates showed methicillin susceptibility and were characterized as spa type t272/CC121, Panton-Valentine leukocidin negative, and hlB positive. Most of the coagulase-negative staphylococci showed resistance to fusidic acid and beta-lactams, and multidrug resistance was identified especially among S. epidermidis isolates. The mecA gene was detected in 20 isolates of the species S. fleurettii and S. epidermidis, associated with the blaZ gene in one S. epidermidis isolate. Five antimicrobial resistance genes were detected in one S. epidermidis isolate (mecA,dfrA,dfrG,aac6'-aph2'', and ant4). Our results highlight that wild rabbits are reservoirs or "temporary hosts" of Staphylococcus species with zoonotic potential, some of them carrying relevant antimicrobial resistances. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-01 2020-07-01T00:00:00Z 2021-03-31T17:08:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7620 |
url |
http://hdl.handle.net/10400.18/7620 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
J Food Prot. 2020 Jul 1;83(7):1110-1114. doi: 10.4315/0362-028X.JFP-19-423. 0362-028X 10.4315/0362-028X.JFP-19-423 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Association for Food Protection |
publisher.none.fl_str_mv |
International Association for Food Protection |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132167272398848 |