Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence

Detalhes bibliográficos
Autor(a) principal: Carvalho, Nuno
Data de Publicação: 2024
Outros Autores: Carolino, Elisabete, Ferreira, Margarida, Coelho, Hélder, Santos, Catarina Rolo, Barreira, Ana Lúcia, Henriques, Susana, Cardoso, Carlos, Moita, Luís, Costa, Paulo Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/17100
Resumo: This study is part of a research project which seeks to evaluate the immune–inflammatory response in AA, without specific funding. The cost of mast cell granule protein determination was supported by the Joaquim Chaves Lab.
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spelling Tryptase in acute appendicitis: unveiling allergic connections through compelling evidenceAcute appendicitisAllergyHistamineHypersensitivity type I reactionMastocytesPathophysiologySerotoninTryptaseThis study is part of a research project which seeks to evaluate the immune–inflammatory response in AA, without specific funding. The cost of mast cell granule protein determination was supported by the Joaquim Chaves Lab.The aetiology of acute appendicitis (AA), the most frequent abdominal surgical emergency, is still unclarified. Recent epidemiologic, clinical, and laboratory data point to an allergic component in the pathophysiology of AA. Mastocytes participate in the Th2 immune response, releasing inflammatory mediators from their granules upon stimulation by IgE-specific antigens. Among the well-known mediators are histamine, serotonin, and tryptase, which are responsible for the clinical manifestations of allergies. We conducted a prospective single-centre study to measure histamine and serotonin (commercial ELISA kit) and tryptase (ImmunoCAP System) concentrations in appendicular lavage fluid (ALF) and serum. Consecutive patients presenting to the emergency department with a clinical diagnosis of AA were enrolled: 22 patients with phlegmonous AA and 24 with gangrenous AA The control group was composed of 14 patients referred for colectomy for colon malignancy. Appendectomy was performed during colectomy. Tryptase levels were strikingly different between histological groups, both in ALF and serum (p < 0.001); ALF levels were higher than serum levels. Tryptase concentrations in ALF were 109 times higher in phlegmonous AA (APA) (796.8 (194.1-980.5) pg/mL) and 114 times higher in gangrenous AA (AGA) (837.4 (272.6-1075.1) pg/mL) than in the control group (7.3 (4.5-10.3) pg/mL. For the diagnosis of AA, the discriminative power of serum tryptase concentration was good (AUC = 0.825), but the discriminative power was weak (AUC = 0.559) for the differential diagnosis between APA and AGA. Mastocytes are involved in AA during clinical presentations of both phlegmonous and gangrenous appendicitis, and no significant differences in concentration were found. No differences were found in serum and ALF concentrations of histamine and serotonin between histological groups. Due to their short half-lives, these might have elapsed by the time the samples were collected. In future research, these determinations should be made immediately after appendectomy. Our findings confirm the hypersensitivity type I reaction as an event occurring in the pathogenesis of AA: tryptase levels in ALF and serum were higher among patients with AA when compared to the control group, which is in line with a Th2 immune response and supports the concept of the presence of an allergic reaction in the pathogenesis of acute appendicitis. Our results, if confirmed, may have clinical implications for the treatment of AA.MDPIRCIPLCarvalho, NunoCarolino, ElisabeteFerreira, MargaridaCoelho, HélderSantos, Catarina RoloBarreira, Ana LúciaHenriques, SusanaCardoso, CarlosMoita, LuísCosta, Paulo Matos2024-02-12T11:22:14Z2024-012024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/17100engCarvalho N, Carolino E, Ferreira M, Coelho H, Santos CR, Barreira AL, et al. Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence. Int J Mol Sci. 2024;25(3):1645.10.3390/ijms25031645info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-21T02:17:14Zoai:repositorio.ipl.pt:10400.21/17100Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:38:12.968121Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
title Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
spellingShingle Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
Carvalho, Nuno
Acute appendicitis
Allergy
Histamine
Hypersensitivity type I reaction
Mastocytes
Pathophysiology
Serotonin
Tryptase
title_short Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
title_full Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
title_fullStr Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
title_full_unstemmed Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
title_sort Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence
author Carvalho, Nuno
author_facet Carvalho, Nuno
Carolino, Elisabete
Ferreira, Margarida
Coelho, Hélder
Santos, Catarina Rolo
Barreira, Ana Lúcia
Henriques, Susana
Cardoso, Carlos
Moita, Luís
Costa, Paulo Matos
author_role author
author2 Carolino, Elisabete
Ferreira, Margarida
Coelho, Hélder
Santos, Catarina Rolo
Barreira, Ana Lúcia
Henriques, Susana
Cardoso, Carlos
Moita, Luís
Costa, Paulo Matos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Carvalho, Nuno
Carolino, Elisabete
Ferreira, Margarida
Coelho, Hélder
Santos, Catarina Rolo
Barreira, Ana Lúcia
Henriques, Susana
Cardoso, Carlos
Moita, Luís
Costa, Paulo Matos
dc.subject.por.fl_str_mv Acute appendicitis
Allergy
Histamine
Hypersensitivity type I reaction
Mastocytes
Pathophysiology
Serotonin
Tryptase
topic Acute appendicitis
Allergy
Histamine
Hypersensitivity type I reaction
Mastocytes
Pathophysiology
Serotonin
Tryptase
description This study is part of a research project which seeks to evaluate the immune–inflammatory response in AA, without specific funding. The cost of mast cell granule protein determination was supported by the Joaquim Chaves Lab.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-12T11:22:14Z
2024-01
2024-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/17100
url http://hdl.handle.net/10400.21/17100
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalho N, Carolino E, Ferreira M, Coelho H, Santos CR, Barreira AL, et al. Tryptase in acute appendicitis: unveiling allergic connections through compelling evidence. Int J Mol Sci. 2024;25(3):1645.
10.3390/ijms25031645
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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