RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics

Detalhes bibliográficos
Autor(a) principal: Thole, Vera
Data de Publicação: 2019
Outros Autores: Bassard, Jean Etienne, Ramírez-González, Ricardo, Trick, Martin, Ghasemi Afshar, Bijan, Breitel, Dario, Hill, Lionel, Foito, Alexandre, Shepherd, Louise, Freitag, Sabine, Nunes Dos Santos, Claúdia, Menezes, Regina, Banãdos, Pilar, Naesby, Michael, Wang, Liangsheng, Sorokin, Artem, Tikhonova, Olga, Shelenga, Tatiana, Stewart, Derek, Vain, Philippe, Martin, Cathie
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/90873
Resumo: This research was funded by the European Union Framework Program 7, Project BacHBerry [FP7–613793]. The authors also acknowledge support from the Institute Strategic Programmes ‘Designing Future Wheat’ (BB/P016855/1), ‘Understanding and Exploiting Plant and Microbial Secondary Metabolism’ (BB/J004596/1) and ‘Molecules from Nature’ (BB/P012523/1) from the UK Biotechnology and Biological Sciences Research Council to the John Innes Centre and the European funded COST ACTION FA1106 QualityFruit. VT, PV and CM have also received funding from the European Union’s Horizon 2020 research and innovation programme through the TomGEM project under grant agreement No. 679796. The funding bodies had no role in the design of the study, collection, analysis and interpretation of data nor in writing the manuscript.
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spelling RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics13 berry fruit speciesAnthocyaninbHLHde novo assemblyFruit ripeningGene expression analysisMYBRNA-seqTranscription factorsWDRBiotechnologyGeneticsThis research was funded by the European Union Framework Program 7, Project BacHBerry [FP7–613793]. The authors also acknowledge support from the Institute Strategic Programmes ‘Designing Future Wheat’ (BB/P016855/1), ‘Understanding and Exploiting Plant and Microbial Secondary Metabolism’ (BB/J004596/1) and ‘Molecules from Nature’ (BB/P012523/1) from the UK Biotechnology and Biological Sciences Research Council to the John Innes Centre and the European funded COST ACTION FA1106 QualityFruit. VT, PV and CM have also received funding from the European Union’s Horizon 2020 research and innovation programme through the TomGEM project under grant agreement No. 679796. The funding bodies had no role in the design of the study, collection, analysis and interpretation of data nor in writing the manuscript.Background: Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild and cultivated berry fruit species are often limited, despite their value in underpinning the in-depth study of metabolic pathways, fruit ripening as well as in the identification of genotypes rich in bioactive compounds. Results: To access the genetic diversity of wild and cultivated berry fruit species that accumulate high levels of phenolic compounds in their fleshy berry(-like) fruits, we selected 13 species from Europe, South America and Asia representing eight genera, seven families and seven orders within three clades of the kingdom Plantae. RNA from either ripe fruits (ten species) or three ripening stages (two species) as well as leaf RNA (one species) were used to construct, assemble and analyse de novo transcriptomes. The transcriptome sequences are deposited in the BacHBerryGEN database (http://jicbio.nbi.ac.uk/berries) and were used, as a proof of concept, via its BLAST portal (http://jicbio.nbi.ac.uk/berries/blast.html) to identify candidate genes involved in the biosynthesis of phenylpropanoid compounds. Genes encoding regulatory proteins of the anthocyanin biosynthetic pathway (MYB and basic helix-loop-helix (bHLH) transcription factors and WD40 repeat proteins) were isolated using the transcriptomic resources of wild blackberry (Rubus genevieri) and cultivated red raspberry (Rubus idaeus cv. Prestige) and were shown to activate anthocyanin synthesis in Nicotiana benthamiana. Expression patterns of candidate flavonoid gene transcripts were also studied across three fruit developmental stages via the BacHBerryEXP gene expression browser (http://www.bachberryexp.com) in R. genevieri and R. idaeus cv. Prestige. Conclusions: We report a transcriptome resource that includes data for a wide range of berry(-like) fruit species that has been developed for gene identification and functional analysis to assist in berry fruit improvement. These resources will enable investigations of metabolic processes in berries beyond the phenylpropanoid biosynthetic pathway analysed in this study. The RNA-seq data will be useful for studies of berry fruit development and to select wild plant species useful for plant breeding purposes.Centro de Estudos de Doenças Crónicas (CEDOC)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNThole, VeraBassard, Jean EtienneRamírez-González, RicardoTrick, MartinGhasemi Afshar, BijanBreitel, DarioHill, LionelFoito, AlexandreShepherd, LouiseFreitag, SabineNunes Dos Santos, ClaúdiaMenezes, ReginaBanãdos, PilarNaesby, MichaelWang, LiangshengSorokin, ArtemTikhonova, OlgaShelenga, TatianaStewart, DerekVain, PhilippeMartin, Cathie2020-01-07T23:33:28Z2019-12-192019-12-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/90873eng1471-2164PURE: 16212510https://doi.org/10.1186/s12864-019-6183-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:40:21Zoai:run.unl.pt:10362/90873Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:12.175144Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
title RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
spellingShingle RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
Thole, Vera
13 berry fruit species
Anthocyanin
bHLH
de novo assembly
Fruit ripening
Gene expression analysis
MYB
RNA-seq
Transcription factors
WDR
Biotechnology
Genetics
title_short RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
title_full RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
title_fullStr RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
title_full_unstemmed RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
title_sort RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics
author Thole, Vera
author_facet Thole, Vera
Bassard, Jean Etienne
Ramírez-González, Ricardo
Trick, Martin
Ghasemi Afshar, Bijan
Breitel, Dario
Hill, Lionel
Foito, Alexandre
Shepherd, Louise
Freitag, Sabine
Nunes Dos Santos, Claúdia
Menezes, Regina
Banãdos, Pilar
Naesby, Michael
Wang, Liangsheng
Sorokin, Artem
Tikhonova, Olga
Shelenga, Tatiana
Stewart, Derek
Vain, Philippe
Martin, Cathie
author_role author
author2 Bassard, Jean Etienne
Ramírez-González, Ricardo
Trick, Martin
Ghasemi Afshar, Bijan
Breitel, Dario
Hill, Lionel
Foito, Alexandre
Shepherd, Louise
Freitag, Sabine
Nunes Dos Santos, Claúdia
Menezes, Regina
Banãdos, Pilar
Naesby, Michael
Wang, Liangsheng
Sorokin, Artem
Tikhonova, Olga
Shelenga, Tatiana
Stewart, Derek
Vain, Philippe
Martin, Cathie
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Thole, Vera
Bassard, Jean Etienne
Ramírez-González, Ricardo
Trick, Martin
Ghasemi Afshar, Bijan
Breitel, Dario
Hill, Lionel
Foito, Alexandre
Shepherd, Louise
Freitag, Sabine
Nunes Dos Santos, Claúdia
Menezes, Regina
Banãdos, Pilar
Naesby, Michael
Wang, Liangsheng
Sorokin, Artem
Tikhonova, Olga
Shelenga, Tatiana
Stewart, Derek
Vain, Philippe
Martin, Cathie
dc.subject.por.fl_str_mv 13 berry fruit species
Anthocyanin
bHLH
de novo assembly
Fruit ripening
Gene expression analysis
MYB
RNA-seq
Transcription factors
WDR
Biotechnology
Genetics
topic 13 berry fruit species
Anthocyanin
bHLH
de novo assembly
Fruit ripening
Gene expression analysis
MYB
RNA-seq
Transcription factors
WDR
Biotechnology
Genetics
description This research was funded by the European Union Framework Program 7, Project BacHBerry [FP7–613793]. The authors also acknowledge support from the Institute Strategic Programmes ‘Designing Future Wheat’ (BB/P016855/1), ‘Understanding and Exploiting Plant and Microbial Secondary Metabolism’ (BB/J004596/1) and ‘Molecules from Nature’ (BB/P012523/1) from the UK Biotechnology and Biological Sciences Research Council to the John Innes Centre and the European funded COST ACTION FA1106 QualityFruit. VT, PV and CM have also received funding from the European Union’s Horizon 2020 research and innovation programme through the TomGEM project under grant agreement No. 679796. The funding bodies had no role in the design of the study, collection, analysis and interpretation of data nor in writing the manuscript.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-19
2019-12-19T00:00:00Z
2020-01-07T23:33:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/90873
url http://hdl.handle.net/10362/90873
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-2164
PURE: 16212510
https://doi.org/10.1186/s12864-019-6183-2
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eu_rights_str_mv openAccess
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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