Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/2037 |
Resumo: | An accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optimal extraction parameters. Validation of the overall methodology was performed by spiking assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight were obtained. The established method was applied in edible tissues of three commonly consumed and commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight) and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and carcinogenic effects of PAHs in food. |
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Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extractionPolycyclic aromatic hydrocarbonsFishMicrowave-assisted extractionResponse surface methodologyLiquid chromatographyAn accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optimal extraction parameters. Validation of the overall methodology was performed by spiking assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight were obtained. The established method was applied in edible tissues of three commonly consumed and commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight) and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and carcinogenic effects of PAHs in food.ElsevierRepositório Científico do Instituto Politécnico do PortoRamalhosa, Maria JoãoPaíga, PaulaMorais, SimoneSousa, Ana M. M.Gonçalves, Maria PilarDelerue-Matos, CristinaOliveira, M. Beatriz P. P.2013-09-30T11:02:04Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/2037eng0308-814610.101/j.foodchem.2012.04.078info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:41:34Zoai:recipp.ipp.pt:10400.22/2037Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:23:08.850712Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
title |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
spellingShingle |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction Ramalhosa, Maria João Polycyclic aromatic hydrocarbons Fish Microwave-assisted extraction Response surface methodology Liquid chromatography |
title_short |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
title_full |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
title_fullStr |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
title_full_unstemmed |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
title_sort |
Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction |
author |
Ramalhosa, Maria João |
author_facet |
Ramalhosa, Maria João Paíga, Paula Morais, Simone Sousa, Ana M. M. Gonçalves, Maria Pilar Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. |
author_role |
author |
author2 |
Paíga, Paula Morais, Simone Sousa, Ana M. M. Gonçalves, Maria Pilar Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Ramalhosa, Maria João Paíga, Paula Morais, Simone Sousa, Ana M. M. Gonçalves, Maria Pilar Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. |
dc.subject.por.fl_str_mv |
Polycyclic aromatic hydrocarbons Fish Microwave-assisted extraction Response surface methodology Liquid chromatography |
topic |
Polycyclic aromatic hydrocarbons Fish Microwave-assisted extraction Response surface methodology Liquid chromatography |
description |
An accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optimal extraction parameters. Validation of the overall methodology was performed by spiking assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight were obtained. The established method was applied in edible tissues of three commonly consumed and commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight) and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and carcinogenic effects of PAHs in food. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2013-09-30T11:02:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/2037 |
url |
http://hdl.handle.net/10400.22/2037 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0308-8146 10.101/j.foodchem.2012.04.078 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131327762530304 |