Production and characterization of bioactive peptides from blue mussel shells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43905 |
Resumo: | Mussels, a popular global seafood choice, and the disposal of large amounts of mussel shells raise environmental concerns, prompting the industry to explore two main approaches, extracting calcium carbonate and valorizing the organic matrix composed of proteins, for more sustainable waste utilization. In this study we aimed to extract and characterize bioactive peptides from the blue mussel (Mytilus edulis) shells, contributing to an added-value upcycling of this waste stream. In terms of demineralization, the overall yield of extraction was 84.6%, as for the solubilization step, it was 53.2%, and for the hydrolysis, it obtained a global yield of 59.1%. The blue mussel shells peptides obtained exhibited antioxidant activities, namely 3.98 ± 0.41 µmol Trolox Equivalent/mg protein for ABTS method (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) and 4.12 ± 0.22 µmol Trolox Equivalent/mg protein for ORAC method (oxygen radical absorbance capacity). The antihypertensive activity, determined by Angiotensin-converting enzyme (ACE) inhibitory activity, resulted in an IC50 of 805.7 ± 18.0 µg protein/mL. The peptide size composition of the obtained fractions characterized through High Pressure Size Exclusion Chromatography (HPSEC) revealed most of the peptides in the range between 1 and 3 kDa and under 1 kDa. Following the gastrointestinal simulation, the acquired peptides demonstrated antioxidant properties at the end of digestion, specifically 7.06 ± 1.96 µmol Trolox equivalents/mg of protein using the ABTS method and 4.38 ± 0.36 µmol Trolox equivalents/mg of protein using the ORAC method. This pioneering study allowed us to obtain a good yield of peptides from the blue mussel shell and demonstrated their bioactive potential. |
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Production and characterization of bioactive peptides from blue mussel shellsBioactive peptidesEnzymatic hydrolysisBlue mussel shell (mytilus edulis)Antioxidant activityAntihypertensive activityAntimicrobial activityPéptidos bioativosHidrólise enzimáticaConcha de mexilhão-azul (mytilus edulis)Atividade antioxidanteAtividade anti-hipertensivaAtividade antimicrobianaDomínio/Área Científica::Ciências Naturais::Ciências BiológicasMussels, a popular global seafood choice, and the disposal of large amounts of mussel shells raise environmental concerns, prompting the industry to explore two main approaches, extracting calcium carbonate and valorizing the organic matrix composed of proteins, for more sustainable waste utilization. In this study we aimed to extract and characterize bioactive peptides from the blue mussel (Mytilus edulis) shells, contributing to an added-value upcycling of this waste stream. In terms of demineralization, the overall yield of extraction was 84.6%, as for the solubilization step, it was 53.2%, and for the hydrolysis, it obtained a global yield of 59.1%. The blue mussel shells peptides obtained exhibited antioxidant activities, namely 3.98 ± 0.41 µmol Trolox Equivalent/mg protein for ABTS method (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) and 4.12 ± 0.22 µmol Trolox Equivalent/mg protein for ORAC method (oxygen radical absorbance capacity). The antihypertensive activity, determined by Angiotensin-converting enzyme (ACE) inhibitory activity, resulted in an IC50 of 805.7 ± 18.0 µg protein/mL. The peptide size composition of the obtained fractions characterized through High Pressure Size Exclusion Chromatography (HPSEC) revealed most of the peptides in the range between 1 and 3 kDa and under 1 kDa. Following the gastrointestinal simulation, the acquired peptides demonstrated antioxidant properties at the end of digestion, specifically 7.06 ± 1.96 µmol Trolox equivalents/mg of protein using the ABTS method and 4.38 ± 0.36 µmol Trolox equivalents/mg of protein using the ORAC method. This pioneering study allowed us to obtain a good yield of peptides from the blue mussel shell and demonstrated their bioactive potential.Os mexilhões, são uma escolha muito popular de frutos do mar e o descarte de grandes quantidades de conchas de mexilhão levanta preocupações ambientais, levando a indústria a explorar duas abordagens principais para o seu reaproveitamento, a extração de carbonato de cálcio e a valorização da sua matriz orgânica, composta por proteínas, para uma utilização mais sustentável dos resíduos. Este estudo tem como objetivo extrair e caracterizar péptidos bioativos das conchas de mexilhão-azul (Mytilus edulis). Para extrair os péptidos bioativos das cascas dos mexilhões foram realizadas três etapas, a saber, desmineralização, solubilização e hidrólise enzimática. Em termos de desmineralização o rendimento global de extração foi de 84.6%, para a etapa de solubilização foi de 53.2% e para a hidrólise obteve-se um rendimento global de 59.1%. Os péptidos obtidos a partir da concha do mexilhão azul, exibiram atividades antioxidantes, nomeadamente 3.98 ± 0.41 µmol de equivalente Trolox/mg de proteína para o método de ABTS e 4.12 ± 0.22 µmol de equivalente de Trolox/mg de proteína para o método de ORAC. A atividade anti-hipertensiva, determinada pelo método iACE, resultou em IC50 de 805.7 ± 18.0 µg proteína/mL. A distribuição do tamanho dos péptidos obtidos foi caracterizada por cromatografia de exclusão de tamanho, e revelou que a maioria dos péptidos se encontrava entre 1 e 3 kDa e abaixo de 1 kDa. Após simulação gastrointestinal, os peptídeos adquiridos demonstraram propriedades antioxidantes, medindo especificamente 7.06 ± 1.96 µmol equivalentes de Trolox/mg de proteína usando o método ABTS e 4.38 ± 0.36 µmol equivalentes de Trolox/mg de proteína usando o método ORAC. Este estudo pioneiro permitiu extrair péptidos bioativos da concha do mexilhão-azul e demonstra o seu potencial bioativo.Pintado, Maria Manuela EstevezCoscueta, Ezequiel RicardoTorrecilla, Maria Paula JaureguiVeritati - Repositório Institucional da Universidade Católica PortuguesaFerreira, Daniela Marisa Vale2023-12-142023-112025-02-01T00:00:00Z2023-12-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.14/43905TID:203443080enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-20T01:32:36Zoai:repositorio.ucp.pt:10400.14/43905Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:37:58.455663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production and characterization of bioactive peptides from blue mussel shells |
title |
Production and characterization of bioactive peptides from blue mussel shells |
spellingShingle |
Production and characterization of bioactive peptides from blue mussel shells Ferreira, Daniela Marisa Vale Bioactive peptides Enzymatic hydrolysis Blue mussel shell (mytilus edulis) Antioxidant activity Antihypertensive activity Antimicrobial activity Péptidos bioativos Hidrólise enzimática Concha de mexilhão-azul (mytilus edulis) Atividade antioxidante Atividade anti-hipertensiva Atividade antimicrobiana Domínio/Área Científica::Ciências Naturais::Ciências Biológicas |
title_short |
Production and characterization of bioactive peptides from blue mussel shells |
title_full |
Production and characterization of bioactive peptides from blue mussel shells |
title_fullStr |
Production and characterization of bioactive peptides from blue mussel shells |
title_full_unstemmed |
Production and characterization of bioactive peptides from blue mussel shells |
title_sort |
Production and characterization of bioactive peptides from blue mussel shells |
author |
Ferreira, Daniela Marisa Vale |
author_facet |
Ferreira, Daniela Marisa Vale |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pintado, Maria Manuela Estevez Coscueta, Ezequiel Ricardo Torrecilla, Maria Paula Jauregui Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ferreira, Daniela Marisa Vale |
dc.subject.por.fl_str_mv |
Bioactive peptides Enzymatic hydrolysis Blue mussel shell (mytilus edulis) Antioxidant activity Antihypertensive activity Antimicrobial activity Péptidos bioativos Hidrólise enzimática Concha de mexilhão-azul (mytilus edulis) Atividade antioxidante Atividade anti-hipertensiva Atividade antimicrobiana Domínio/Área Científica::Ciências Naturais::Ciências Biológicas |
topic |
Bioactive peptides Enzymatic hydrolysis Blue mussel shell (mytilus edulis) Antioxidant activity Antihypertensive activity Antimicrobial activity Péptidos bioativos Hidrólise enzimática Concha de mexilhão-azul (mytilus edulis) Atividade antioxidante Atividade anti-hipertensiva Atividade antimicrobiana Domínio/Área Científica::Ciências Naturais::Ciências Biológicas |
description |
Mussels, a popular global seafood choice, and the disposal of large amounts of mussel shells raise environmental concerns, prompting the industry to explore two main approaches, extracting calcium carbonate and valorizing the organic matrix composed of proteins, for more sustainable waste utilization. In this study we aimed to extract and characterize bioactive peptides from the blue mussel (Mytilus edulis) shells, contributing to an added-value upcycling of this waste stream. In terms of demineralization, the overall yield of extraction was 84.6%, as for the solubilization step, it was 53.2%, and for the hydrolysis, it obtained a global yield of 59.1%. The blue mussel shells peptides obtained exhibited antioxidant activities, namely 3.98 ± 0.41 µmol Trolox Equivalent/mg protein for ABTS method (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) and 4.12 ± 0.22 µmol Trolox Equivalent/mg protein for ORAC method (oxygen radical absorbance capacity). The antihypertensive activity, determined by Angiotensin-converting enzyme (ACE) inhibitory activity, resulted in an IC50 of 805.7 ± 18.0 µg protein/mL. The peptide size composition of the obtained fractions characterized through High Pressure Size Exclusion Chromatography (HPSEC) revealed most of the peptides in the range between 1 and 3 kDa and under 1 kDa. Following the gastrointestinal simulation, the acquired peptides demonstrated antioxidant properties at the end of digestion, specifically 7.06 ± 1.96 µmol Trolox equivalents/mg of protein using the ABTS method and 4.38 ± 0.36 µmol Trolox equivalents/mg of protein using the ORAC method. This pioneering study allowed us to obtain a good yield of peptides from the blue mussel shell and demonstrated their bioactive potential. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-14 2023-11 2023-12-14T00:00:00Z 2025-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43905 TID:203443080 |
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http://hdl.handle.net/10400.14/43905 |
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TID:203443080 |
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eng |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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