Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/13080 |
Resumo: | Scale removal in fish triggers a damage-repair program to re-establish the lost epidermis and scale and an associated local immune response. In mammals, chronic stress is known to delay wound healing and to modulate the cutaneous stress axis, but this is unstudied in teleost fish the most successful extant vertebrates. The present study was designed to test the hypothesis that chronic stress impairs cutaneous repair in teleost fish as a consequence of suppression of the immune response. The hypothesis was tested by removing the scales and damaging the skin on one side of the body of fish previously exposed for 4 weeks to a chronic crowding stress and then evaluating cutaneous repair for 1 week. Scale removal caused the loss of the epidermis although at 3 days it was re-established. At this stage the basement membrane was significantly thicker (p = 0.038) and the hypodermis was significantly thinner (p = 0.016) in the regenerating skin of stressed fish relative to the control fish. At 3 days, stressed fish also had a significantly lower plasma osmolality (p = 0.015) than control fish indicative of reduced barrier function. Chronic stress caused a significant down-regulation of the glucocorticoid receptor (gr) in skin before damage (time 0, p = 0.005) and of star at 3 and 7 days (p < 0.05) after regeneration relative to control fish. In regenerating skin key transcripts of cutaneous repair, pcna, colival and nunp9, and the inflammatory response, tgf beta 1, mpo and crtac2, were down-regulated (p < 0.05) by chronic stress. Irrespective of chronic stress and in contrast to intact skin many hyper pigmented masses, putative melanomacrophages, infiltrated the epidermis of regenerating skin. This study reveals that chronic stress suppresses the local immune response to scale removal and impairs the expression of key transcripts of wound healing. Elements of the stress axis were identified and modulated by chronic stress during cutaneous repair in gilthead seabream skin. |
id |
RCAP_e9186476d0fb6e4b3d499463c7e9a5ff |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/13080 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.)Messenger-RnaCellular responsesScale regenerationGrowth factorsTeleost fishDifferential expressionGlucocorticoid-receptorDicentrarchus-LabraxBasement membraneSkin regenerationBarrier functionCutaneous wound healingImmune responseMelanomacrophage centersPeripheral stress axisScale removal in fish triggers a damage-repair program to re-establish the lost epidermis and scale and an associated local immune response. In mammals, chronic stress is known to delay wound healing and to modulate the cutaneous stress axis, but this is unstudied in teleost fish the most successful extant vertebrates. The present study was designed to test the hypothesis that chronic stress impairs cutaneous repair in teleost fish as a consequence of suppression of the immune response. The hypothesis was tested by removing the scales and damaging the skin on one side of the body of fish previously exposed for 4 weeks to a chronic crowding stress and then evaluating cutaneous repair for 1 week. Scale removal caused the loss of the epidermis although at 3 days it was re-established. At this stage the basement membrane was significantly thicker (p = 0.038) and the hypodermis was significantly thinner (p = 0.016) in the regenerating skin of stressed fish relative to the control fish. At 3 days, stressed fish also had a significantly lower plasma osmolality (p = 0.015) than control fish indicative of reduced barrier function. Chronic stress caused a significant down-regulation of the glucocorticoid receptor (gr) in skin before damage (time 0, p = 0.005) and of star at 3 and 7 days (p < 0.05) after regeneration relative to control fish. In regenerating skin key transcripts of cutaneous repair, pcna, colival and nunp9, and the inflammatory response, tgf beta 1, mpo and crtac2, were down-regulated (p < 0.05) by chronic stress. Irrespective of chronic stress and in contrast to intact skin many hyper pigmented masses, putative melanomacrophages, infiltrated the epidermis of regenerating skin. This study reveals that chronic stress suppresses the local immune response to scale removal and impairs the expression of key transcripts of wound healing. Elements of the stress axis were identified and modulated by chronic stress during cutaneous repair in gilthead seabream skin.European Regional Development Fund through COMPETEPortuguese Foundation for Science and Technology (FCT) [CCMAR/Multi/04326/2013, PTDC/MAR/ 122296/2010]FCT [UID/Multi/04326/ 2013, SFRH/BPD/79105/2011]Ministry of Science and Higher Education, PolandElsevierSapientiaMateus, AnaAnjos, LilianaCardoso, João CRPower, Deborah2019-11-20T15:07:31Z2017-072017-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/13080eng0161-589010.1016/j.molimm.2017.04.008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:25:07Zoai:sapientia.ualg.pt:10400.1/13080Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:04:17.570402Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
title |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
spellingShingle |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) Mateus, Ana Messenger-Rna Cellular responses Scale regeneration Growth factors Teleost fish Differential expression Glucocorticoid-receptor Dicentrarchus-Labrax Basement membrane Skin regeneration Barrier function Cutaneous wound healing Immune response Melanomacrophage centers Peripheral stress axis |
title_short |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
title_full |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
title_fullStr |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
title_full_unstemmed |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
title_sort |
Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.) |
author |
Mateus, Ana |
author_facet |
Mateus, Ana Anjos, Liliana Cardoso, João CR Power, Deborah |
author_role |
author |
author2 |
Anjos, Liliana Cardoso, João CR Power, Deborah |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Mateus, Ana Anjos, Liliana Cardoso, João CR Power, Deborah |
dc.subject.por.fl_str_mv |
Messenger-Rna Cellular responses Scale regeneration Growth factors Teleost fish Differential expression Glucocorticoid-receptor Dicentrarchus-Labrax Basement membrane Skin regeneration Barrier function Cutaneous wound healing Immune response Melanomacrophage centers Peripheral stress axis |
topic |
Messenger-Rna Cellular responses Scale regeneration Growth factors Teleost fish Differential expression Glucocorticoid-receptor Dicentrarchus-Labrax Basement membrane Skin regeneration Barrier function Cutaneous wound healing Immune response Melanomacrophage centers Peripheral stress axis |
description |
Scale removal in fish triggers a damage-repair program to re-establish the lost epidermis and scale and an associated local immune response. In mammals, chronic stress is known to delay wound healing and to modulate the cutaneous stress axis, but this is unstudied in teleost fish the most successful extant vertebrates. The present study was designed to test the hypothesis that chronic stress impairs cutaneous repair in teleost fish as a consequence of suppression of the immune response. The hypothesis was tested by removing the scales and damaging the skin on one side of the body of fish previously exposed for 4 weeks to a chronic crowding stress and then evaluating cutaneous repair for 1 week. Scale removal caused the loss of the epidermis although at 3 days it was re-established. At this stage the basement membrane was significantly thicker (p = 0.038) and the hypodermis was significantly thinner (p = 0.016) in the regenerating skin of stressed fish relative to the control fish. At 3 days, stressed fish also had a significantly lower plasma osmolality (p = 0.015) than control fish indicative of reduced barrier function. Chronic stress caused a significant down-regulation of the glucocorticoid receptor (gr) in skin before damage (time 0, p = 0.005) and of star at 3 and 7 days (p < 0.05) after regeneration relative to control fish. In regenerating skin key transcripts of cutaneous repair, pcna, colival and nunp9, and the inflammatory response, tgf beta 1, mpo and crtac2, were down-regulated (p < 0.05) by chronic stress. Irrespective of chronic stress and in contrast to intact skin many hyper pigmented masses, putative melanomacrophages, infiltrated the epidermis of regenerating skin. This study reveals that chronic stress suppresses the local immune response to scale removal and impairs the expression of key transcripts of wound healing. Elements of the stress axis were identified and modulated by chronic stress during cutaneous repair in gilthead seabream skin. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07 2017-07-01T00:00:00Z 2019-11-20T15:07:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/13080 |
url |
http://hdl.handle.net/10400.1/13080 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0161-5890 10.1016/j.molimm.2017.04.008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133279515836416 |