Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection

Detalhes bibliográficos
Autor(a) principal: Temporão, Adriana
Data de Publicação: 2021
Outros Autores: Sanches-Vaz, Margarida, Luís, Rafael, Nunes-Cabaço, Helena, Smith, Terry K., Prudêncio, Miguel, Figueiredo, Luisa M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56428
Resumo: © 2021 Temporão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection© 2021 Temporão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malaria, a disease caused by Plasmodium parasites, remains a major threat to public health globally. It is the most common disease in patients with sleeping sickness, another parasitic illness, caused by Trypanosoma brucei. We have previously shown that a T. brucei infection impairs a secondary P. berghei liver infection and decreases malaria severity in mice. However, whether this effect requires an active trypanosome infection remained unknown. Here, we show that Plasmodium liver infection can also be inhibited by the serum of a mouse previously infected by T. brucei and by total protein lysates of this kinetoplastid. Biochemical characterisation showed that the anti-Plasmodium activity of the total T. brucei lysates depends on its protein fraction, but is independent of the abundant variant surface glycoprotein. Finally, we found that the protein(s) responsible for the inhibition of Plasmodium infection is/are present within a fraction of ~350 proteins that are excreted to the bloodstream of the host. We conclude that the defence mechanism developed by trypanosomes against Plasmodium relies on protein excretion. This study opens the door to the identification of novel antiplasmodial intervention strategies.This work was supported by the Howard Hughes Medical Institute (ref. 55007419) awarded to LMF, by Fundação para a Ciência e Tecnologia (PD/BD/138891/2018) awarded to AT, (PD/BD/105838/2018) awarded to MSV, (CEECIND/03322/2018) awarded to LMF, (CEECIND/03539/2017) awarded to MP, and by the Wellcome Trust (094476/Z/10/Z) awarded to TKS.PLOSRepositório da Universidade de LisboaTemporão, AdrianaSanches-Vaz, MargaridaLuís, RafaelNunes-Cabaço, HelenaSmith, Terry K.Prudêncio, MiguelFigueiredo, Luisa M.2023-02-23T15:19:20Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/56428engPLoS Negl Trop Dis . 2021 Oct 29;15(10):e00099121935-272710.1371/journal.pntd.00099121935-2735info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:19:39Zoai:repositorio.ul.pt:10451/56428Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:19:39Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
title Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
spellingShingle Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
Temporão, Adriana
title_short Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
title_full Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
title_fullStr Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
title_full_unstemmed Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
title_sort Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection
author Temporão, Adriana
author_facet Temporão, Adriana
Sanches-Vaz, Margarida
Luís, Rafael
Nunes-Cabaço, Helena
Smith, Terry K.
Prudêncio, Miguel
Figueiredo, Luisa M.
author_role author
author2 Sanches-Vaz, Margarida
Luís, Rafael
Nunes-Cabaço, Helena
Smith, Terry K.
Prudêncio, Miguel
Figueiredo, Luisa M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Temporão, Adriana
Sanches-Vaz, Margarida
Luís, Rafael
Nunes-Cabaço, Helena
Smith, Terry K.
Prudêncio, Miguel
Figueiredo, Luisa M.
description © 2021 Temporão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2023-02-23T15:19:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56428
url http://hdl.handle.net/10451/56428
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS Negl Trop Dis . 2021 Oct 29;15(10):e0009912
1935-2727
10.1371/journal.pntd.0009912
1935-2735
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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