Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100

Detalhes bibliográficos
Autor(a) principal: Dias, Nicolina
Data de Publicação: 2003
Outros Autores: Mortara, Renato A., Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1466
Resumo: Non-ionic surfactants such as Triton X-100 have been widely used in industrial processing and in cleaning products for almost 50 years, being effective and economic emulsifying, wetting agents, dispersants and solubilizers. Cleaning products containing these surfactants are disposed of mainly by discharge into wastewater, which receives biological treatment in wastewater treatment systems. However, surface-active agents interact with eukaryotic cell membranes leading to biological damage at high concentrations. Tetrahymena pyriformis was used here as model organism to assess the effects of Triton X-100 through a series of in vitro cytotoxicity tests. Growth rates and morphological changes were, by their simplicity and reproducibility, the simplest toxicological assays. Cytoskeleton analysis seemed to be related with phagocytosis rate. Viability was evaluated by two different tests. Calcein AM/EthD-1 was used to assess T. pyriformis membrane damage during the 48-h experiment. The colorimetric MTT assay proved to be highly sensitive even at very short periods of Triton X-100 exposure. Tests performed in this study included simple and fast bioassays that provide overall information on the morphological and physiological state of cells exposed to different non-lytic and lytic concentrations of Triton X-100.
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spelling Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100Cytotoxicity assessmentIn vitroTetrahymena pyriformisTriton X-100Science & TechnologyNon-ionic surfactants such as Triton X-100 have been widely used in industrial processing and in cleaning products for almost 50 years, being effective and economic emulsifying, wetting agents, dispersants and solubilizers. Cleaning products containing these surfactants are disposed of mainly by discharge into wastewater, which receives biological treatment in wastewater treatment systems. However, surface-active agents interact with eukaryotic cell membranes leading to biological damage at high concentrations. Tetrahymena pyriformis was used here as model organism to assess the effects of Triton X-100 through a series of in vitro cytotoxicity tests. Growth rates and morphological changes were, by their simplicity and reproducibility, the simplest toxicological assays. Cytoskeleton analysis seemed to be related with phagocytosis rate. Viability was evaluated by two different tests. Calcein AM/EthD-1 was used to assess T. pyriformis membrane damage during the 48-h experiment. The colorimetric MTT assay proved to be highly sensitive even at very short periods of Triton X-100 exposure. Tests performed in this study included simple and fast bioassays that provide overall information on the morphological and physiological state of cells exposed to different non-lytic and lytic concentrations of Triton X-100.Fundação para a Ciência e a Tecnologia (FCT) - PRAXIS XXI/BD/20328/99 - Instituto de Biotecnologia e Química Fina (IBQF). Fundação de Amparo à Pesquisa do Estado de SP (FAPESP). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Revista de Atualização Médica ( RAM).Elsevier ScienceUniversidade do MinhoDias, NicolinaMortara, Renato A.Lima, Nelson20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1466eng"Toxicology in vitro". ISSN 0887-2333. 17:3 (2003) 357–366.0887-233310.1016/S0887-2333(03)00023-712781214info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:46Zoai:repositorium.sdum.uminho.pt:1822/1466Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:40.769977Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
title Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
spellingShingle Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
Dias, Nicolina
Cytotoxicity assessment
In vitro
Tetrahymena pyriformis
Triton X-100
Science & Technology
title_short Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
title_full Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
title_fullStr Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
title_full_unstemmed Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
title_sort Morphological and physiological changes in tetrahymena pyriformis for the in vitro cytotoxicity assessment of triton X-100
author Dias, Nicolina
author_facet Dias, Nicolina
Mortara, Renato A.
Lima, Nelson
author_role author
author2 Mortara, Renato A.
Lima, Nelson
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Dias, Nicolina
Mortara, Renato A.
Lima, Nelson
dc.subject.por.fl_str_mv Cytotoxicity assessment
In vitro
Tetrahymena pyriformis
Triton X-100
Science & Technology
topic Cytotoxicity assessment
In vitro
Tetrahymena pyriformis
Triton X-100
Science & Technology
description Non-ionic surfactants such as Triton X-100 have been widely used in industrial processing and in cleaning products for almost 50 years, being effective and economic emulsifying, wetting agents, dispersants and solubilizers. Cleaning products containing these surfactants are disposed of mainly by discharge into wastewater, which receives biological treatment in wastewater treatment systems. However, surface-active agents interact with eukaryotic cell membranes leading to biological damage at high concentrations. Tetrahymena pyriformis was used here as model organism to assess the effects of Triton X-100 through a series of in vitro cytotoxicity tests. Growth rates and morphological changes were, by their simplicity and reproducibility, the simplest toxicological assays. Cytoskeleton analysis seemed to be related with phagocytosis rate. Viability was evaluated by two different tests. Calcein AM/EthD-1 was used to assess T. pyriformis membrane damage during the 48-h experiment. The colorimetric MTT assay proved to be highly sensitive even at very short periods of Triton X-100 exposure. Tests performed in this study included simple and fast bioassays that provide overall information on the morphological and physiological state of cells exposed to different non-lytic and lytic concentrations of Triton X-100.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1466
url http://hdl.handle.net/1822/1466
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Toxicology in vitro". ISSN 0887-2333. 17:3 (2003) 357–366.
0887-2333
10.1016/S0887-2333(03)00023-7
12781214
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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