Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay

Detalhes bibliográficos
Autor(a) principal: Moreira, David
Data de Publicação: 2024
Outros Autores: Alexandre, Daniela, Miranda, André, Lourenço, Pedro, Baptista, Pedro V., Tomaz, Cândida, Lu, Yi, Cruz, Carla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/173336
Resumo: Funding Information: This study was developed within the scope of the CICS-UBI projects 10.54499/UIDB/00709/2020 and 10.54499/UIDP/00709/2020; LA/P/0140/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES; “Bolsa de Investigação em Oncologia Dr. Rocha Alves do Núcleo Regional do Centro da Liga Portuguesa Contra o Cancro”, Instruct-ERIC Pilot R&D application ID 2473, and PAPILOMA ref. CENTRO-01-0145-FEDER-181235. David Moreira, André Miranda, and Daniela Alexandre acknowledge doctoral fellowship grants from FCT https://doi.org/10.54499/2023.02001.BD, https://doi.org/10.54499/2021.07695.BD and https://sciproj.ptcris.pt/80505DFA, respectively. Pedro Lourenço acknowledges a fellowship grant financed by FCT to CICS-UBI (ref. UIDP/00709/2020). Publisher Copyright: © 2024 by the authors.
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spelling Detecting mir-155-3p through a Molecular Beacon Bead-Based Assaylung cancermicrofluidicsmir-155-3pmolecular beaconAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistrySDG 3 - Good Health and Well-beingFunding Information: This study was developed within the scope of the CICS-UBI projects 10.54499/UIDB/00709/2020 and 10.54499/UIDP/00709/2020; LA/P/0140/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES; “Bolsa de Investigação em Oncologia Dr. Rocha Alves do Núcleo Regional do Centro da Liga Portuguesa Contra o Cancro”, Instruct-ERIC Pilot R&D application ID 2473, and PAPILOMA ref. CENTRO-01-0145-FEDER-181235. David Moreira, André Miranda, and Daniela Alexandre acknowledge doctoral fellowship grants from FCT https://doi.org/10.54499/2023.02001.BD, https://doi.org/10.54499/2021.07695.BD and https://sciproj.ptcris.pt/80505DFA, respectively. Pedro Lourenço acknowledges a fellowship grant financed by FCT to CICS-UBI (ref. UIDP/00709/2020). Publisher Copyright: © 2024 by the authors.Lung cancer (LC) is recognized as one of the most prevalent and lethal cancers worldwide, underscoring an urgent need for innovative diagnostic and therapeutic approaches. MicroRNAs (miRNAs) have emerged as promising biomarkers for several diseases and their progression, such as LC. However, traditional methods for detecting and quantifying miRNAs, such as PCR, are time-consuming and expensive. Herein, we used a molecular beacon (MB) bead-based assay immobilized in a microfluidic device to detect miR-155-3p, which is frequently overexpressed in LC. The assay relies on the fluorescence enhancement of the MB upon binding to the target miRNA via Watson and Crick complementarity, resulting in a conformational change from a stem–loop to a linear structure, thereby bringing apart the fluorophores at each end. This assay was performed on a microfluidic platform enabling rapid and straightforward target detection. We successfully detected miR-155-3p in a saline solution, obtaining a limit of detection (LOD) of 42 nM. Furthermore, we evaluated the method’s performance in more complex biological samples, including A549 cells’ total RNA and peripheral blood mononuclear cells (PBMCs) spiked with the target miRNA. We achieved satisfactory recovery rates, especially in A549 cells’ total RNA.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNMoreira, DavidAlexandre, DanielaMiranda, AndréLourenço, PedroBaptista, Pedro V.Tomaz, CândidaLu, YiCruz, Carla2024-10-10T22:39:13Z2024-07-032024-07-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/173336eng1420-3049PURE: 99281614https://doi.org/10.3390/molecules29133182info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-18T01:41:33Zoai:run.unl.pt:10362/173336Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-18T01:41:33Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
title Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
spellingShingle Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
Moreira, David
lung cancer
microfluidics
mir-155-3p
molecular beacon
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
title_short Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
title_full Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
title_fullStr Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
title_full_unstemmed Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
title_sort Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay
author Moreira, David
author_facet Moreira, David
Alexandre, Daniela
Miranda, André
Lourenço, Pedro
Baptista, Pedro V.
Tomaz, Cândida
Lu, Yi
Cruz, Carla
author_role author
author2 Alexandre, Daniela
Miranda, André
Lourenço, Pedro
Baptista, Pedro V.
Tomaz, Cândida
Lu, Yi
Cruz, Carla
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Moreira, David
Alexandre, Daniela
Miranda, André
Lourenço, Pedro
Baptista, Pedro V.
Tomaz, Cândida
Lu, Yi
Cruz, Carla
dc.subject.por.fl_str_mv lung cancer
microfluidics
mir-155-3p
molecular beacon
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
topic lung cancer
microfluidics
mir-155-3p
molecular beacon
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
SDG 3 - Good Health and Well-being
description Funding Information: This study was developed within the scope of the CICS-UBI projects 10.54499/UIDB/00709/2020 and 10.54499/UIDP/00709/2020; LA/P/0140/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES; “Bolsa de Investigação em Oncologia Dr. Rocha Alves do Núcleo Regional do Centro da Liga Portuguesa Contra o Cancro”, Instruct-ERIC Pilot R&D application ID 2473, and PAPILOMA ref. CENTRO-01-0145-FEDER-181235. David Moreira, André Miranda, and Daniela Alexandre acknowledge doctoral fellowship grants from FCT https://doi.org/10.54499/2023.02001.BD, https://doi.org/10.54499/2021.07695.BD and https://sciproj.ptcris.pt/80505DFA, respectively. Pedro Lourenço acknowledges a fellowship grant financed by FCT to CICS-UBI (ref. UIDP/00709/2020). Publisher Copyright: © 2024 by the authors.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-10T22:39:13Z
2024-07-03
2024-07-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/173336
url http://hdl.handle.net/10362/173336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
PURE: 99281614
https://doi.org/10.3390/molecules29133182
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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