A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms

Detalhes bibliográficos
Autor(a) principal: Oliveira, Adilia P.
Data de Publicação: 2008
Outros Autores: Pampulha, Maria Elisa, Bennett, James P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/5519
Resumo: We evaluated the changes of some soil microbiological characteristics due to the use of transgenic maize expressing Bacillus thuringiensis (Bt) toxin. A two-year field experiment was conducted (2003 and 2004). Two lines of transgenic Bt maize that express the Cry1Ab protein (event 176 and MON 810) and their near-isogenic non-Bt lines were used. Rhizosphere and non-rhizosphere soils were collected and measurements were performed during the maize cultural cycle and immediately at pre-harvest. Key soil microbiological parameters measured included the numbers of culturable aerobic bacteria, including actinomycetes, and fungi, the activity of dehydrogenase and nitrogenase enzymes and ATP content. There were clear seasonal effects in the microbial parameters as evidenced by the consistent changes in sampling dates across the two years. Differences in the measured variables were also observed between rhizosphere and non-rhizosphere soils. However, under our field conditions, the presence of Bt maize did not cause, in a general way, changes in the microbial populations of the soil or in the activity of the microbial community
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spelling A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganismsBt maizeculturable soil microbiotadehydrogenase activitynitrogenase activityATP contentWe evaluated the changes of some soil microbiological characteristics due to the use of transgenic maize expressing Bacillus thuringiensis (Bt) toxin. A two-year field experiment was conducted (2003 and 2004). Two lines of transgenic Bt maize that express the Cry1Ab protein (event 176 and MON 810) and their near-isogenic non-Bt lines were used. Rhizosphere and non-rhizosphere soils were collected and measurements were performed during the maize cultural cycle and immediately at pre-harvest. Key soil microbiological parameters measured included the numbers of culturable aerobic bacteria, including actinomycetes, and fungi, the activity of dehydrogenase and nitrogenase enzymes and ATP content. There were clear seasonal effects in the microbial parameters as evidenced by the consistent changes in sampling dates across the two years. Differences in the measured variables were also observed between rhizosphere and non-rhizosphere soils. However, under our field conditions, the presence of Bt maize did not cause, in a general way, changes in the microbial populations of the soil or in the activity of the microbial communityElsevierRepositório da Universidade de LisboaOliveira, Adilia P.Pampulha, Maria ElisaBennett, James P.2013-05-29T10:26:58Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/5519eng"Science of the Total Environment". ISSN 0048-9697. 405 (2008) 351-3570048-9697info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T19:05:23Zoai:repositorio.ul.pt:10400.5/5519Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T19:05:23Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
title A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
spellingShingle A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
Oliveira, Adilia P.
Bt maize
culturable soil microbiota
dehydrogenase activity
nitrogenase activity
ATP content
title_short A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
title_full A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
title_fullStr A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
title_full_unstemmed A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
title_sort A two-year field study with transgenic Bacillus thuringiensis maize: effects on soil microorganisms
author Oliveira, Adilia P.
author_facet Oliveira, Adilia P.
Pampulha, Maria Elisa
Bennett, James P.
author_role author
author2 Pampulha, Maria Elisa
Bennett, James P.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Oliveira, Adilia P.
Pampulha, Maria Elisa
Bennett, James P.
dc.subject.por.fl_str_mv Bt maize
culturable soil microbiota
dehydrogenase activity
nitrogenase activity
ATP content
topic Bt maize
culturable soil microbiota
dehydrogenase activity
nitrogenase activity
ATP content
description We evaluated the changes of some soil microbiological characteristics due to the use of transgenic maize expressing Bacillus thuringiensis (Bt) toxin. A two-year field experiment was conducted (2003 and 2004). Two lines of transgenic Bt maize that express the Cry1Ab protein (event 176 and MON 810) and their near-isogenic non-Bt lines were used. Rhizosphere and non-rhizosphere soils were collected and measurements were performed during the maize cultural cycle and immediately at pre-harvest. Key soil microbiological parameters measured included the numbers of culturable aerobic bacteria, including actinomycetes, and fungi, the activity of dehydrogenase and nitrogenase enzymes and ATP content. There were clear seasonal effects in the microbial parameters as evidenced by the consistent changes in sampling dates across the two years. Differences in the measured variables were also observed between rhizosphere and non-rhizosphere soils. However, under our field conditions, the presence of Bt maize did not cause, in a general way, changes in the microbial populations of the soil or in the activity of the microbial community
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2013-05-29T10:26:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/5519
url http://hdl.handle.net/10400.5/5519
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Science of the Total Environment". ISSN 0048-9697. 405 (2008) 351-357
0048-9697
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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