Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/20.500.12207/5424 |
Resumo: | Euphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype |
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Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotypeEuphorbia heterophyllaPest controlExtensive crop areasImazamoxControlo de infestantesHerbicidaEuphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotypeNature2022-05-05T12:12:40Z2019-01-01T00:00:00Z2019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12207/5424enghttps://doi.org/10.1038/s41598-019-51682-zRojano-Blanco, AntoniaPortugal, JoãoPalma-Bautista, CandelarioAlcantara-de la Cruz, RicardoTorra, JoelAlcantara, EstebanDe Prado, Rafaelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-06-23T07:47:47Zoai:repositorio.ipbeja.pt:20.500.12207/5424Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T14:59:39.336552Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
title |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
spellingShingle |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype Rojano-Blanco, Antonia Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
title_short |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
title_full |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
title_fullStr |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
title_full_unstemmed |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
title_sort |
Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype |
author |
Rojano-Blanco, Antonia |
author_facet |
Rojano-Blanco, Antonia Portugal, João Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael |
author_role |
author |
author2 |
Portugal, João Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rojano-Blanco, Antonia Portugal, João Palma-Bautista, Candelario Alcantara-de la Cruz, Ricardo Torra, Joel Alcantara, Esteban De Prado, Rafael |
dc.subject.por.fl_str_mv |
Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
topic |
Euphorbia heterophylla Pest control Extensive crop areas Imazamox Controlo de infestantes Herbicida |
description |
Euphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confrmed by dose-response assays since it required 1250.2g ai ha−1 to reduce the fresh weight by 50% versus 7.4g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from fve diferent chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2019-01-01T00:00:00Z 2022-05-05T12:12:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/20.500.12207/5424 |
url |
http://hdl.handle.net/20.500.12207/5424 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://doi.org/10.1038/s41598-019-51682-z |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature |
publisher.none.fl_str_mv |
Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799129868936413184 |