Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Cristina Alves
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/17810
Resumo: Mestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de Agronomia
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spelling Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)Diplodia sapinaPestalotiopsis spp.Sydowia polysporaITSPinus pineaPortugalMestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de AgronomiaUntil a few years ago, the stone pine (Pinus pinea) was a species affected by few pathogens, but recently shoot blight and death of the shoots have been frequently reported in our country, causing serious concerns for producers and pine nut industry. By hindering the development of cones, this problem affects the production of pine nuts, the main resource of this forest industry. Lately, fungi belonging to different genera have been identified associated with shoot blight of stone pine. Some of these fungi, such as Sydowia polyspora, were unknown in Portugal and others, although present in our country (eg Pestalotiopsis spp.), were usually considered weak or stress parasites, affecting only weakened trees. The main aim of the present work was to contribute for the identification of the fungi associated to shoot blight of stone pine and to clarify their role in the expression of the disease. A total of 46 isolates from seven different sites were analysed. Identification was made based on cultural and morphological characteristics and phylogenetic analysis (ITS, TEF and TUB regions). Isolates of the most relevant species were tested for their pathogenicity. This work allowed to identify several species of fungi associated with shoot blight in stone pine, namely: Diplodia sapinea, Epicoccum nigrum, Pestalotiopsis australis, Pestalotiopsis sp. and Sydowia polyspora. The inoculation of one year-old P. pinea plants under controlled conditions allowed to confirm the pathogenicity of isolates of species D. sapinea and Pestalotiopsis sp.. The role of S. polyspora in the expression of these symptoms was not clarified, however, in the plants inoculated simultaneously with D. sapinea and S. polyspora isolates, the incidence of the disease was much higher than in the plants inoculated with D. sapinea alone, raising the hypothesis that S. polyspora may be potentiating the pathogenicity of D. sapinea. In this work, the fungus S. polyspora was identified for the first time in Portugal and for the first time in stone pine. It should also be pointed out that the isolate belonging to Pestalotiopsis sp., whose pathogenicity was proved in the present work, presents morpho-cultural and genetic characteristics different from those described for other species within this genus, being more aggressive than other known species within this genusISA/ULBragança, Maria Helena PiresRamos, Ana Paula FerreiraRepositório da Universidade de LisboaSilva, Ana Cristina Alves2021-06-01T00:30:15Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/17810TID:203092449engSilva, A.C.A. - Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.). Lisboa: ISA, 2018, 100 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:28Zoai:www.repository.utl.pt:10400.5/17810Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:02:58.625799Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
title Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
spellingShingle Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
Silva, Ana Cristina Alves
Diplodia sapina
Pestalotiopsis spp.
Sydowia polyspora
ITS
Pinus pinea
Portugal
title_short Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
title_full Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
title_fullStr Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
title_full_unstemmed Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
title_sort Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.)
author Silva, Ana Cristina Alves
author_facet Silva, Ana Cristina Alves
author_role author
dc.contributor.none.fl_str_mv Bragança, Maria Helena Pires
Ramos, Ana Paula Ferreira
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Ana Cristina Alves
dc.subject.por.fl_str_mv Diplodia sapina
Pestalotiopsis spp.
Sydowia polyspora
ITS
Pinus pinea
Portugal
topic Diplodia sapina
Pestalotiopsis spp.
Sydowia polyspora
ITS
Pinus pinea
Portugal
description Mestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de Agronomia
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2021-06-01T00:30:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/17810
TID:203092449
url http://hdl.handle.net/10400.5/17810
identifier_str_mv TID:203092449
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, A.C.A. - Contribution to the study of shoot blight disease on stone pine (Pinus pinea L.). Lisboa: ISA, 2018, 100 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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