Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/598 |
Resumo: | Animals have a determined species-specific body size that results from the combined action of hormones and signaling pathways regulating growth rate and duration. In Drosophila, the steroid hormone ecdysone controls developmental transitions, thereby regulating the duration of the growth period. Here we show that ecdysone promotes the growth of imaginal discs in mid-third instar larvae, since imaginal discs from larvae with reduced or no ecdysone synthesis are smaller than wild type due to smaller and fewer cells. We show that insulin-like peptides are produced and secreted normally in larvae with reduced ecdysone synthesis, and upstream components of insulin/insulin-like signaling are activated in their discs. Instead, ecdysone appears to regulate the growth of imaginal discs via Thor/4E-BP, a negative growth regulator downstream of the insulin/insulin-like growth factor/Tor pathways. Discs from larvae with reduced ecdysone synthesis have elevated levels of Thor, while mutations in Thor partially rescue their growth. The regulation of organ growth by ecdysone is evolutionarily conserved in hemimetabolous insects, as shown by our results obtained using Blattella germanica. In summary, our data provide new insights into the relationship between components of the insulin/insulin-like/Tor and ecdysone pathways in the control of organ growth. |
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Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogasterCell proliferationDevelopmentLimb developmentTOR signallingAnimals have a determined species-specific body size that results from the combined action of hormones and signaling pathways regulating growth rate and duration. In Drosophila, the steroid hormone ecdysone controls developmental transitions, thereby regulating the duration of the growth period. Here we show that ecdysone promotes the growth of imaginal discs in mid-third instar larvae, since imaginal discs from larvae with reduced or no ecdysone synthesis are smaller than wild type due to smaller and fewer cells. We show that insulin-like peptides are produced and secreted normally in larvae with reduced ecdysone synthesis, and upstream components of insulin/insulin-like signaling are activated in their discs. Instead, ecdysone appears to regulate the growth of imaginal discs via Thor/4E-BP, a negative growth regulator downstream of the insulin/insulin-like growth factor/Tor pathways. Discs from larvae with reduced ecdysone synthesis have elevated levels of Thor, while mutations in Thor partially rescue their growth. The regulation of organ growth by ecdysone is evolutionarily conserved in hemimetabolous insects, as shown by our results obtained using Blattella germanica. In summary, our data provide new insights into the relationship between components of the insulin/insulin-like/Tor and ecdysone pathways in the control of organ growth.Spanish Ministry of Science and Consolider program grants: (BFU-2008-01884, BFU2011-25986, CSD2007-008-25120, BFU2009-10571 and BES-2009-016077); Departments of Education and Industry of the Basque Government grant: (PI2012/42); Bizkaia County; Instituto Gulbenkian de Ciência/Fundação Calouste Gulbenkian; Fundação Para a Ciência e a Tecnologia fellowship: (SFRH/BD/51181/2010).Nature Publishing GroupARCAHerboso, LeireOliveira, Marisa M.Talamillo, AnaPérez, CoraliaGonzález, MonikaMartín, DavidSutherland, James D.Shingleton, Alexander W.Mirth, Christen K.Barrio, Rosa2016-05-05T10:21:57Z2015-07-222015-07-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/mswordapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://hdl.handle.net/10400.7/598engHerboso, L. et al. Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster. Sci. Rep. 5, 12383; doi: 10.1038/srep12383 (2015).10.1038/srep12383info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:58Zoai:arca.igc.gulbenkian.pt:10400.7/598Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:50.708957Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
title |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
spellingShingle |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster Herboso, Leire Cell proliferation Development Limb development TOR signalling |
title_short |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
title_full |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
title_fullStr |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
title_full_unstemmed |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
title_sort |
Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster |
author |
Herboso, Leire |
author_facet |
Herboso, Leire Oliveira, Marisa M. Talamillo, Ana Pérez, Coralia González, Monika Martín, David Sutherland, James D. Shingleton, Alexander W. Mirth, Christen K. Barrio, Rosa |
author_role |
author |
author2 |
Oliveira, Marisa M. Talamillo, Ana Pérez, Coralia González, Monika Martín, David Sutherland, James D. Shingleton, Alexander W. Mirth, Christen K. Barrio, Rosa |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Herboso, Leire Oliveira, Marisa M. Talamillo, Ana Pérez, Coralia González, Monika Martín, David Sutherland, James D. Shingleton, Alexander W. Mirth, Christen K. Barrio, Rosa |
dc.subject.por.fl_str_mv |
Cell proliferation Development Limb development TOR signalling |
topic |
Cell proliferation Development Limb development TOR signalling |
description |
Animals have a determined species-specific body size that results from the combined action of hormones and signaling pathways regulating growth rate and duration. In Drosophila, the steroid hormone ecdysone controls developmental transitions, thereby regulating the duration of the growth period. Here we show that ecdysone promotes the growth of imaginal discs in mid-third instar larvae, since imaginal discs from larvae with reduced or no ecdysone synthesis are smaller than wild type due to smaller and fewer cells. We show that insulin-like peptides are produced and secreted normally in larvae with reduced ecdysone synthesis, and upstream components of insulin/insulin-like signaling are activated in their discs. Instead, ecdysone appears to regulate the growth of imaginal discs via Thor/4E-BP, a negative growth regulator downstream of the insulin/insulin-like growth factor/Tor pathways. Discs from larvae with reduced ecdysone synthesis have elevated levels of Thor, while mutations in Thor partially rescue their growth. The regulation of organ growth by ecdysone is evolutionarily conserved in hemimetabolous insects, as shown by our results obtained using Blattella germanica. In summary, our data provide new insights into the relationship between components of the insulin/insulin-like/Tor and ecdysone pathways in the control of organ growth. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-22 2015-07-22T00:00:00Z 2016-05-05T10:21:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/598 |
url |
http://hdl.handle.net/10400.7/598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Herboso, L. et al. Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster. Sci. Rep. 5, 12383; doi: 10.1038/srep12383 (2015). 10.1038/srep12383 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/msword application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130573757743104 |