Modification upon aging of the density of presynaptic modulation systems in the hippocampus
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/4689 https://doi.org/10.1016/j.neurobiolaging.2008.01.003 |
Resumo: | Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards, whereas the densities of GABAergic (vGAT) only decreased after 24 months. Inhibitory A1 and CB1 receptor density tended to decrease with aging, whereas facilitatory mGluR5 and P2Y1 receptor density was roughly constant and facilitatory A2A receptor density increased at 18-24 months. Thus aging causes an imbalance of excitatory versus inhibitory nerve terminal markers and causes a predominant decrease of inhibitory rather than facilitatory presynaptic modulation systems. |
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Modification upon aging of the density of presynaptic modulation systems in the hippocampusNerve terminalsDensityAdenosineMetabotropicGlutamateAcetylcholineSynaptophysinDifferent presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards, whereas the densities of GABAergic (vGAT) only decreased after 24 months. Inhibitory A1 and CB1 receptor density tended to decrease with aging, whereas facilitatory mGluR5 and P2Y1 receptor density was roughly constant and facilitatory A2A receptor density increased at 18-24 months. Thus aging causes an imbalance of excitatory versus inhibitory nerve terminal markers and causes a predominant decrease of inhibitory rather than facilitatory presynaptic modulation systems.http://www.sciencedirect.com/science/article/B6T09-4S02T4M-1/1/e64b5e4577dafc00487393f333c02a462008-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/4689http://hdl.handle.net/10316/4689https://doi.org/10.1016/j.neurobiolaging.2008.01.003engNeurobiology of Aging. In Press, Corrected Proof:Canas, Paula M.Duarte, João M. N.Rodrigues, Ricardo J.Köfalvi, AttilaCunha, Rodrigo A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:49:18Zoai:estudogeral.uc.pt:10316/4689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:34.009611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
title |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
spellingShingle |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus Canas, Paula M. Nerve terminals Density Adenosine Metabotropic Glutamate Acetylcholine Synaptophysin |
title_short |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
title_full |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
title_fullStr |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
title_full_unstemmed |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
title_sort |
Modification upon aging of the density of presynaptic modulation systems in the hippocampus |
author |
Canas, Paula M. |
author_facet |
Canas, Paula M. Duarte, João M. N. Rodrigues, Ricardo J. Köfalvi, Attila Cunha, Rodrigo A. |
author_role |
author |
author2 |
Duarte, João M. N. Rodrigues, Ricardo J. Köfalvi, Attila Cunha, Rodrigo A. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Canas, Paula M. Duarte, João M. N. Rodrigues, Ricardo J. Köfalvi, Attila Cunha, Rodrigo A. |
dc.subject.por.fl_str_mv |
Nerve terminals Density Adenosine Metabotropic Glutamate Acetylcholine Synaptophysin |
topic |
Nerve terminals Density Adenosine Metabotropic Glutamate Acetylcholine Synaptophysin |
description |
Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards, whereas the densities of GABAergic (vGAT) only decreased after 24 months. Inhibitory A1 and CB1 receptor density tended to decrease with aging, whereas facilitatory mGluR5 and P2Y1 receptor density was roughly constant and facilitatory A2A receptor density increased at 18-24 months. Thus aging causes an imbalance of excitatory versus inhibitory nerve terminal markers and causes a predominant decrease of inhibitory rather than facilitatory presynaptic modulation systems. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/4689 http://hdl.handle.net/10316/4689 https://doi.org/10.1016/j.neurobiolaging.2008.01.003 |
url |
http://hdl.handle.net/10316/4689 https://doi.org/10.1016/j.neurobiolaging.2008.01.003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neurobiology of Aging. In Press, Corrected Proof: |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133707796217856 |