A new cuticle scale hydrolysing protease from beauveria brongniartii

Detalhes bibliográficos
Autor(a) principal: Erlacher, Angelika
Data de Publicação: 2006
Outros Autores: Sousa, Fernanda, Schroeder, M., Jus, S., Kokol, V., Paulo, Artur Cavaco, Gübitz, Georg M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/12954
Resumo: From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen analysis. The C/N ratio of released material turned out to be a good parameter to determine the site of action of proteases on fibres. Compared to other enzymes, the fungal protease preferentially hydrolyzed cuticle scales and has thus a potential for antishrinking pre-treatment of wool fabrics.
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spelling A new cuticle scale hydrolysing protease from beauveria brongniartiiWoolCuticleProteaseBeauveria brongniartiiScience & TechnologyFrom a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen analysis. The C/N ratio of released material turned out to be a good parameter to determine the site of action of proteases on fibres. Compared to other enzymes, the fungal protease preferentially hydrolyzed cuticle scales and has thus a potential for antishrinking pre-treatment of wool fabrics.SpringerUniversidade do MinhoErlacher, AngelikaSousa, FernandaSchroeder, M.Jus, S.Kokol, V.Paulo, Artur CavacoGübitz, Georg M.2006-052006-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12954eng0141-549210.1007/s10529-006-9047-116791724info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:54:11Zoai:repositorium.sdum.uminho.pt:1822/12954Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:44.207327Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new cuticle scale hydrolysing protease from beauveria brongniartii
title A new cuticle scale hydrolysing protease from beauveria brongniartii
spellingShingle A new cuticle scale hydrolysing protease from beauveria brongniartii
Erlacher, Angelika
Wool
Cuticle
Protease
Beauveria brongniartii
Science & Technology
title_short A new cuticle scale hydrolysing protease from beauveria brongniartii
title_full A new cuticle scale hydrolysing protease from beauveria brongniartii
title_fullStr A new cuticle scale hydrolysing protease from beauveria brongniartii
title_full_unstemmed A new cuticle scale hydrolysing protease from beauveria brongniartii
title_sort A new cuticle scale hydrolysing protease from beauveria brongniartii
author Erlacher, Angelika
author_facet Erlacher, Angelika
Sousa, Fernanda
Schroeder, M.
Jus, S.
Kokol, V.
Paulo, Artur Cavaco
Gübitz, Georg M.
author_role author
author2 Sousa, Fernanda
Schroeder, M.
Jus, S.
Kokol, V.
Paulo, Artur Cavaco
Gübitz, Georg M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Erlacher, Angelika
Sousa, Fernanda
Schroeder, M.
Jus, S.
Kokol, V.
Paulo, Artur Cavaco
Gübitz, Georg M.
dc.subject.por.fl_str_mv Wool
Cuticle
Protease
Beauveria brongniartii
Science & Technology
topic Wool
Cuticle
Protease
Beauveria brongniartii
Science & Technology
description From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen analysis. The C/N ratio of released material turned out to be a good parameter to determine the site of action of proteases on fibres. Compared to other enzymes, the fungal protease preferentially hydrolyzed cuticle scales and has thus a potential for antishrinking pre-treatment of wool fabrics.
publishDate 2006
dc.date.none.fl_str_mv 2006-05
2006-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/12954
url http://hdl.handle.net/1822/12954
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0141-5492
10.1007/s10529-006-9047-1
16791724
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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