Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/28186 |
Resumo: | Olive (Olea europaea subsp. europaea var. europaea L.) comprises several cultivars with reduced capacity to be propagated due its recalcitrant behaviour upon adventitious rooting (AR) stimulus. This prevents their propagation and consequently their availability in the nurseries. By overcoming the difficult-to-root behaviour it will be possible to increase the number of cultivars available for new orchards and consequently taking profit of it on final products differentiation. There are many protocols used in vegetative propagation to induce AR based on auxins, a group of phytohormones largely known as involved in many processes of plant development, including root initiation and development. However, most of these protocols are still based on “trial/error” approaches, where several variables needs to be tested. This happens because genetic control underlying AR is not completely elucidated. Auxins are mainly synthesized in young leaves and apical meristem of shoots and roots. The major auxin distribution is regulated by transport from cell to cell, known as polar auxin transport (PAT). PAT is mediated by three main classes of membrane auxin transporters, the auxin resistant 1/like aux1 (AUX/LAX), the ATP binding cassette subfamily B (ABCB/MDR/PGP) and the pin-formed (PIN) carriers. The PIN gene family encodes a subgroup of auxin efflux carriers shown to be involved in various developmental processes, including lateral/adventitious root formation, in several plant species. To date, PIN genes have been identified in 31 plant species by genome-wide approaches, however, there is still no information regarding its identification in olive. The recent publication of O. europaea genome allowed us to perform the identification of all members belonging to PIN gene family in this species (OePIN). Our work aims to characterize OePIN family, as well as, to investigate the involvement of its members during AR. The expression profile study of OePIN members during AR in IBA-induced in vitro cultured microshoots of cv. ‘Galega vulgar’, attempting to understand if the hard-rooting behaviour of this cultivar might be related with a disturbance in auxin transport. Additionally, reactive oxygen species (ROS) as key signaling molecules that regulate growth and development and coordinate responses to biotic and abiotic stresses in plants are also being analysed in IBA-treated and non-treated explants. Also, cells from phloem, cortex and sub-epidermis are being isolated and gene expression will be performed on these cells in order to find out which cells are responsible for the formation of adventitious roots. |
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Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L.adventitious rootingmicropropagationoliveauxin carrier genesOlive (Olea europaea subsp. europaea var. europaea L.) comprises several cultivars with reduced capacity to be propagated due its recalcitrant behaviour upon adventitious rooting (AR) stimulus. This prevents their propagation and consequently their availability in the nurseries. By overcoming the difficult-to-root behaviour it will be possible to increase the number of cultivars available for new orchards and consequently taking profit of it on final products differentiation. There are many protocols used in vegetative propagation to induce AR based on auxins, a group of phytohormones largely known as involved in many processes of plant development, including root initiation and development. However, most of these protocols are still based on “trial/error” approaches, where several variables needs to be tested. This happens because genetic control underlying AR is not completely elucidated. Auxins are mainly synthesized in young leaves and apical meristem of shoots and roots. The major auxin distribution is regulated by transport from cell to cell, known as polar auxin transport (PAT). PAT is mediated by three main classes of membrane auxin transporters, the auxin resistant 1/like aux1 (AUX/LAX), the ATP binding cassette subfamily B (ABCB/MDR/PGP) and the pin-formed (PIN) carriers. The PIN gene family encodes a subgroup of auxin efflux carriers shown to be involved in various developmental processes, including lateral/adventitious root formation, in several plant species. To date, PIN genes have been identified in 31 plant species by genome-wide approaches, however, there is still no information regarding its identification in olive. The recent publication of O. europaea genome allowed us to perform the identification of all members belonging to PIN gene family in this species (OePIN). Our work aims to characterize OePIN family, as well as, to investigate the involvement of its members during AR. The expression profile study of OePIN members during AR in IBA-induced in vitro cultured microshoots of cv. ‘Galega vulgar’, attempting to understand if the hard-rooting behaviour of this cultivar might be related with a disturbance in auxin transport. Additionally, reactive oxygen species (ROS) as key signaling molecules that regulate growth and development and coordinate responses to biotic and abiotic stresses in plants are also being analysed in IBA-treated and non-treated explants. Also, cells from phloem, cortex and sub-epidermis are being isolated and gene expression will be performed on these cells in order to find out which cells are responsible for the formation of adventitious roots.2020-10-23T11:05:52Z2020-10-232019-06-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28186http://hdl.handle.net/10174/28186eng. Jornadas MED – Mediterranean Institute for Agriculture, Environment and Development, Universidade de Évora (Évora - Portugal)naonaosimndhcardoso@uevora.ptapeixe@uevora.ptVelada, IsabelCardoso, HéliaPeixe, Augustoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:24:26Zoai:dspace.uevora.pt:10174/28186Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:10.433470Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
title |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
spellingShingle |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. Velada, Isabel adventitious rooting micropropagation olive auxin carrier genes |
title_short |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
title_full |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
title_fullStr |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
title_full_unstemmed |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
title_sort |
Genome-wide analysis and expression profile of PIN-formed auxin carrier genes during in vitro IBA-induced adventitious rooting in Olea europaea L. |
author |
Velada, Isabel |
author_facet |
Velada, Isabel Cardoso, Hélia Peixe, Augusto |
author_role |
author |
author2 |
Cardoso, Hélia Peixe, Augusto |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Velada, Isabel Cardoso, Hélia Peixe, Augusto |
dc.subject.por.fl_str_mv |
adventitious rooting micropropagation olive auxin carrier genes |
topic |
adventitious rooting micropropagation olive auxin carrier genes |
description |
Olive (Olea europaea subsp. europaea var. europaea L.) comprises several cultivars with reduced capacity to be propagated due its recalcitrant behaviour upon adventitious rooting (AR) stimulus. This prevents their propagation and consequently their availability in the nurseries. By overcoming the difficult-to-root behaviour it will be possible to increase the number of cultivars available for new orchards and consequently taking profit of it on final products differentiation. There are many protocols used in vegetative propagation to induce AR based on auxins, a group of phytohormones largely known as involved in many processes of plant development, including root initiation and development. However, most of these protocols are still based on “trial/error” approaches, where several variables needs to be tested. This happens because genetic control underlying AR is not completely elucidated. Auxins are mainly synthesized in young leaves and apical meristem of shoots and roots. The major auxin distribution is regulated by transport from cell to cell, known as polar auxin transport (PAT). PAT is mediated by three main classes of membrane auxin transporters, the auxin resistant 1/like aux1 (AUX/LAX), the ATP binding cassette subfamily B (ABCB/MDR/PGP) and the pin-formed (PIN) carriers. The PIN gene family encodes a subgroup of auxin efflux carriers shown to be involved in various developmental processes, including lateral/adventitious root formation, in several plant species. To date, PIN genes have been identified in 31 plant species by genome-wide approaches, however, there is still no information regarding its identification in olive. The recent publication of O. europaea genome allowed us to perform the identification of all members belonging to PIN gene family in this species (OePIN). Our work aims to characterize OePIN family, as well as, to investigate the involvement of its members during AR. The expression profile study of OePIN members during AR in IBA-induced in vitro cultured microshoots of cv. ‘Galega vulgar’, attempting to understand if the hard-rooting behaviour of this cultivar might be related with a disturbance in auxin transport. Additionally, reactive oxygen species (ROS) as key signaling molecules that regulate growth and development and coordinate responses to biotic and abiotic stresses in plants are also being analysed in IBA-treated and non-treated explants. Also, cells from phloem, cortex and sub-epidermis are being isolated and gene expression will be performed on these cells in order to find out which cells are responsible for the formation of adventitious roots. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-27T00:00:00Z 2020-10-23T11:05:52Z 2020-10-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/28186 http://hdl.handle.net/10174/28186 |
url |
http://hdl.handle.net/10174/28186 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
. Jornadas MED – Mediterranean Institute for Agriculture, Environment and Development, Universidade de Évora (Évora - Portugal) nao nao sim nd hcardoso@uevora.pt apeixe@uevora.pt |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136664169218048 |