Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/12265 |
Resumo: | Although the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. This work addresses the production of a Fusarium solani pisi cutinase by a recombinant Saccharomyces cerevisiae strain induced through the use of a galactose promoter. It was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthesis of the enzymes required for galactose intake, acetate production occurred. The on-line detection of acetate in the medium might prove useful for the control and the supervision of recombinant protein production processes using yeast. The volumes of acid and base added to control the pH throughout the time course of the cultivations were used to calculate an on-line estimator for acetate concentration. |
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Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathwaysSaccharomyces cerevisiaeHeterologous cutinasePhysiologyAcetateAlthough the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. This work addresses the production of a Fusarium solani pisi cutinase by a recombinant Saccharomyces cerevisiae strain induced through the use of a galactose promoter. It was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthesis of the enzymes required for galactose intake, acetate production occurred. The on-line detection of acetate in the medium might prove useful for the control and the supervision of recombinant protein production processes using yeast. The volumes of acid and base added to control the pH throughout the time course of the cultivations were used to calculate an on-line estimator for acetate concentration.ElsevierRCIPLFerreira, Bruno S.Calado, CecíliaKeulen, FrederikvanFonseca, Luís P. P.Cabral, Joaquim M.S.R., Da Fonseca M. M.2020-09-30T12:20:33Z2004-04-082004-04-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/12265engFERREIRA, Bruno S.; [et al] – Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. Journal of Biotechnology. ISSN 0168-1656. Vol. 109, N.º 1-2 (2004), pp. 159-1670168-165610.1016/j.jbiotec.2003.10.033metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:04:50Zoai:repositorio.ipl.pt:10400.21/12265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:23.353599Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
title |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
spellingShingle |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways Ferreira, Bruno S. Saccharomyces cerevisiae Heterologous cutinase Physiology Acetate |
title_short |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
title_full |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
title_fullStr |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
title_full_unstemmed |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
title_sort |
Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways |
author |
Ferreira, Bruno S. |
author_facet |
Ferreira, Bruno S. Calado, Cecília Keulen, Frederikvan Fonseca, Luís P. P. Cabral, Joaquim M.S. R., Da Fonseca M. M. |
author_role |
author |
author2 |
Calado, Cecília Keulen, Frederikvan Fonseca, Luís P. P. Cabral, Joaquim M.S. R., Da Fonseca M. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Ferreira, Bruno S. Calado, Cecília Keulen, Frederikvan Fonseca, Luís P. P. Cabral, Joaquim M.S. R., Da Fonseca M. M. |
dc.subject.por.fl_str_mv |
Saccharomyces cerevisiae Heterologous cutinase Physiology Acetate |
topic |
Saccharomyces cerevisiae Heterologous cutinase Physiology Acetate |
description |
Although the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. This work addresses the production of a Fusarium solani pisi cutinase by a recombinant Saccharomyces cerevisiae strain induced through the use of a galactose promoter. It was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthesis of the enzymes required for galactose intake, acetate production occurred. The on-line detection of acetate in the medium might prove useful for the control and the supervision of recombinant protein production processes using yeast. The volumes of acid and base added to control the pH throughout the time course of the cultivations were used to calculate an on-line estimator for acetate concentration. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-04-08 2004-04-08T00:00:00Z 2020-09-30T12:20:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/12265 |
url |
http://hdl.handle.net/10400.21/12265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FERREIRA, Bruno S.; [et al] – Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. Journal of Biotechnology. ISSN 0168-1656. Vol. 109, N.º 1-2 (2004), pp. 159-167 0168-1656 10.1016/j.jbiotec.2003.10.033 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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