Naked nuclei revisited: p63 immunoexpression

Detalhes bibliográficos
Autor(a) principal: Reis Filho, Jorge S.
Data de Publicação: 2002
Outros Autores: Albergaria, André, Milanezi, Fernanda, Amendoeira, Isabel, Schmitt, Fernando C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1935
Resumo: The presence of naked nuclei (NN) in cytological preparations of fibroadenomas is a well-known finding. Regardless of their importance on the differential diagnosis of fibroadenoma and other benign lesions of the breast, the origin of NN remains elusive. Despite previous efforts to characterize them, the lack of a reliable nuclear marker for myoepithelial cells impaired definitive conclusions. We performed a systematic evaluation of p63 expression in cytological and histological preparations of 10 fibroadenoma specimens. We observed that in histological sections, p63 was restricted to the nuclei of myoepithelial/basal cells in lobules and ducts of normal breast. In fibroadenomas, p63 decorated the nuclei of myoepithelial cells in the periphery of epithelial duct-like formations and slit-like formations. No p63 immunoreactivity was observed in stromal or epithelial cells. In cytological samples, almost all NN and cells surrounding epithelial cell clusters were stained; no stromal cell admixed with fibrillary matrix or epithelial cell was stained with p63. Based on our findings, we strongly suggest that most, if not all, NN are myoepithelial in origin.
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spelling Naked nuclei revisited: p63 immunoexpressionFibroadenomaStromal cellp63Myoepithelial cellNaked nucleiThe presence of naked nuclei (NN) in cytological preparations of fibroadenomas is a well-known finding. Regardless of their importance on the differential diagnosis of fibroadenoma and other benign lesions of the breast, the origin of NN remains elusive. Despite previous efforts to characterize them, the lack of a reliable nuclear marker for myoepithelial cells impaired definitive conclusions. We performed a systematic evaluation of p63 expression in cytological and histological preparations of 10 fibroadenoma specimens. We observed that in histological sections, p63 was restricted to the nuclei of myoepithelial/basal cells in lobules and ducts of normal breast. In fibroadenomas, p63 decorated the nuclei of myoepithelial cells in the periphery of epithelial duct-like formations and slit-like formations. No p63 immunoreactivity was observed in stromal or epithelial cells. In cytological samples, almost all NN and cells surrounding epithelial cell clusters were stained; no stromal cell admixed with fibrillary matrix or epithelial cell was stained with p63. Based on our findings, we strongly suggest that most, if not all, NN are myoepithelial in origin.Fundação para a Ciência e a Tecnologia (FCT) - grant SFRH/BD/5386/2001.WileyUniversidade do MinhoReis Filho, Jorge S.Albergaria, AndréMilanezi, FernandaAmendoeira, IsabelSchmitt, Fernando C.20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1935eng"Diagnostic cytopathology". ISSN 8755-1039. 27:3 (2002) 135-138.8755-103910.1002/dc.1016412203858info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:23Zoai:repositorium.sdum.uminho.pt:1822/1935Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:45.088911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Naked nuclei revisited: p63 immunoexpression
title Naked nuclei revisited: p63 immunoexpression
spellingShingle Naked nuclei revisited: p63 immunoexpression
Reis Filho, Jorge S.
Fibroadenoma
Stromal cell
p63
Myoepithelial cell
Naked nuclei
title_short Naked nuclei revisited: p63 immunoexpression
title_full Naked nuclei revisited: p63 immunoexpression
title_fullStr Naked nuclei revisited: p63 immunoexpression
title_full_unstemmed Naked nuclei revisited: p63 immunoexpression
title_sort Naked nuclei revisited: p63 immunoexpression
author Reis Filho, Jorge S.
author_facet Reis Filho, Jorge S.
Albergaria, André
Milanezi, Fernanda
Amendoeira, Isabel
Schmitt, Fernando C.
author_role author
author2 Albergaria, André
Milanezi, Fernanda
Amendoeira, Isabel
Schmitt, Fernando C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Reis Filho, Jorge S.
Albergaria, André
Milanezi, Fernanda
Amendoeira, Isabel
Schmitt, Fernando C.
dc.subject.por.fl_str_mv Fibroadenoma
Stromal cell
p63
Myoepithelial cell
Naked nuclei
topic Fibroadenoma
Stromal cell
p63
Myoepithelial cell
Naked nuclei
description The presence of naked nuclei (NN) in cytological preparations of fibroadenomas is a well-known finding. Regardless of their importance on the differential diagnosis of fibroadenoma and other benign lesions of the breast, the origin of NN remains elusive. Despite previous efforts to characterize them, the lack of a reliable nuclear marker for myoepithelial cells impaired definitive conclusions. We performed a systematic evaluation of p63 expression in cytological and histological preparations of 10 fibroadenoma specimens. We observed that in histological sections, p63 was restricted to the nuclei of myoepithelial/basal cells in lobules and ducts of normal breast. In fibroadenomas, p63 decorated the nuclei of myoepithelial cells in the periphery of epithelial duct-like formations and slit-like formations. No p63 immunoreactivity was observed in stromal or epithelial cells. In cytological samples, almost all NN and cells surrounding epithelial cell clusters were stained; no stromal cell admixed with fibrillary matrix or epithelial cell was stained with p63. Based on our findings, we strongly suggest that most, if not all, NN are myoepithelial in origin.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1935
url http://hdl.handle.net/1822/1935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Diagnostic cytopathology". ISSN 8755-1039. 27:3 (2002) 135-138.
8755-1039
10.1002/dc.10164
12203858
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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