Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Gaálová-Radochová, Barbora
Data de Publicação: 2023
Outros Autores: Kendra, Samuel, Jordao, Luisa, Kursawe, Laura, Kikhney, Judith, Moter, Annette, Bujdáková, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8667
Resumo: This article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.
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spelling Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureusMethicillin-resistant/sensitive S. aureusBiofilms of C. albicans-MSSA/MRSAResistanceFarnesolEnhancing EffectCell ViabilityMicroscopyThis article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.The natural bioactive molecule farnesol (FAR) is widely studied mainly for its antibiofilm and antimicrobial properties. In addition, it increases the effectiveness of some antimicrobial substances, which makes it interesting for the development of combined therapy. In the present work, the effect of FAR either alone or in combination with oxacillin (OXA) on mixed biofilms formed by clinically relevant pathogens, Candida albicans and Staphylococcus aureus, was studied. S. aureus isolates used for biofilm formation originated from blood cultures and central venous catheters (CVC) were characterized in terms of antimicrobial resistance. The minimal biofilm inhibitory concentration (MBIC50) for FAR of 48 h mixed biofilms formed by the C. albicans and methicillin-sensitive S. aureus (MSSA) was determined to be 125 M, and for the mixed biofilms with methicillin-resistant S. aureus (MRSA) was determined to be 250 M. Treatment of mixed biofilms with OXA (2 mg/mL) showed 4% inhibition; however, the combination of OXA (2 mg/mL) and FAR (300 M) resulted in 80% inhibition of biofilms. In addition, planktonic cells of S. aureus exhibited an increased susceptibility to OXA, cefoxitin and kanamycin in the presence of FAR (150 and 300 M). Scanning electron microscopy (SEM) micrographs confirmed patchy biofilm and lack of candidal hyphae in the samples treated with FAR and FAR/OXA in comparison to control and mixed biofilms treated only with OXA. Intriguingly, in a pilot experiment using fluorescence in situ hybridization (FISH), considerable differences in activity (as indicated by ribosome content) of staphylococcal cells were detected. While the activity rate of the staphylococci in mixed biofilms treated with FAR was high, no FISH-positive signal for staphylococcal cells was found in the biofilm treated with FAR/OXA.This research was funded by the Slovak Research and Development Agency under contracts of SK-PT-18-0006 as part of the Bilateral Cooperation Program (2019–2022), APVV-21-0302 and APVV-18-0075. This work was also supported by the EU Grant number 952398—CEMBO, Call: H2020-WIDESPREAD-05-2020—Twinning.MDPIRepositório Científico do Instituto Nacional de SaúdeGaálová-Radochová, BarboraKendra, SamuelJordao, LuisaKursawe, LauraKikhney, JudithMoter, AnnetteBujdáková, Helena2023-10-06T13:16:08Z2023-02-232023-02-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8667engAntibiotics (Basel). 2023 Feb 23;12(3):441. doi: 10.3390/antibiotics120304412079-638210.3390/antibiotics12030441info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-14T01:31:33Zoai:repositorio.insa.pt:10400.18/8667Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:33:34.170265Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
title Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
spellingShingle Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
Gaálová-Radochová, Barbora
Methicillin-resistant/sensitive S. aureus
Biofilms of C. albicans-MSSA/MRSA
Resistance
Farnesol
Enhancing Effect
Cell Viability
Microscopy
title_short Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
title_full Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
title_fullStr Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
title_full_unstemmed Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
title_sort Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
author Gaálová-Radochová, Barbora
author_facet Gaálová-Radochová, Barbora
Kendra, Samuel
Jordao, Luisa
Kursawe, Laura
Kikhney, Judith
Moter, Annette
Bujdáková, Helena
author_role author
author2 Kendra, Samuel
Jordao, Luisa
Kursawe, Laura
Kikhney, Judith
Moter, Annette
Bujdáková, Helena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Gaálová-Radochová, Barbora
Kendra, Samuel
Jordao, Luisa
Kursawe, Laura
Kikhney, Judith
Moter, Annette
Bujdáková, Helena
dc.subject.por.fl_str_mv Methicillin-resistant/sensitive S. aureus
Biofilms of C. albicans-MSSA/MRSA
Resistance
Farnesol
Enhancing Effect
Cell Viability
Microscopy
topic Methicillin-resistant/sensitive S. aureus
Biofilms of C. albicans-MSSA/MRSA
Resistance
Farnesol
Enhancing Effect
Cell Viability
Microscopy
description This article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-06T13:16:08Z
2023-02-23
2023-02-23T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8667
url http://hdl.handle.net/10400.18/8667
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antibiotics (Basel). 2023 Feb 23;12(3):441. doi: 10.3390/antibiotics12030441
2079-6382
10.3390/antibiotics12030441
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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