Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/18066 |
Resumo: | Saliva is a non-invasive source of biomarkers useful in the study of physiological mechanisms. Moreover, this fluid has diverse functions, among which food perception and ingestion, making it particularly suitable for the study of obesity. The aims of this study were to assess changes in salivary proteome among morbidly obese women, with a view to provide information about mechanisms potentially related to the development of obesity, and to evaluate whether these changes persist after weight loss. Mixed saliva samples from morbidly obese women (N = 18) who had been either subjected (group O-BS) or not (group O) to bariatric surgery and women with normal weight (N = 14; group C) were compared for protein profiles, alpha-amylase abundance and enzymatic activity, and carbonic anhydrase (CA) VI abundance. Differences in salivary obese profiles were observed for 23 different spots. Zinc-alpha-2 glycoprotein-containing spots showed higher abundance in group O only, whereas cystatin S-containing spots presented higher abundance in the two groups of obese subjects. Most of the spots identified as salivary amylase were present at lower levels in group O-BS. With regard to the amylase enzymatic activity, increases were observed for group O and decreases for group O-BS. One interesting finding was the high correlation between levels of CA VI and body mass index in group O, which was not observed for groups O-BS or C. The differences between groups, mainly regarding salivary proteins involved in taste sensitivity and metabolism, point to the potential of using saliva in the study of obesity development. |
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Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or notsalivaobesityprotein profileSaliva is a non-invasive source of biomarkers useful in the study of physiological mechanisms. Moreover, this fluid has diverse functions, among which food perception and ingestion, making it particularly suitable for the study of obesity. The aims of this study were to assess changes in salivary proteome among morbidly obese women, with a view to provide information about mechanisms potentially related to the development of obesity, and to evaluate whether these changes persist after weight loss. Mixed saliva samples from morbidly obese women (N = 18) who had been either subjected (group O-BS) or not (group O) to bariatric surgery and women with normal weight (N = 14; group C) were compared for protein profiles, alpha-amylase abundance and enzymatic activity, and carbonic anhydrase (CA) VI abundance. Differences in salivary obese profiles were observed for 23 different spots. Zinc-alpha-2 glycoprotein-containing spots showed higher abundance in group O only, whereas cystatin S-containing spots presented higher abundance in the two groups of obese subjects. Most of the spots identified as salivary amylase were present at lower levels in group O-BS. With regard to the amylase enzymatic activity, increases were observed for group O and decreases for group O-BS. One interesting finding was the high correlation between levels of CA VI and body mass index in group O, which was not observed for groups O-BS or C. The differences between groups, mainly regarding salivary proteins involved in taste sensitivity and metabolism, point to the potential of using saliva in the study of obesity development.Springer2016-03-15T12:24:24Z2016-03-152015-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/18066http://hdl.handle.net/10174/18066engElsa Lamy, Carla Simões, Lénia Rodrigues, Ana Rodrigues Costa, Rui Vitorino, Francisco Amado, Célia Antunes, Isabel do Carmo “Changes in salivary protein profile in morbid obese women with and without bariatric surgery", Journal of Physiology and Biochemistry”, December 2015, Volume 71, Issue 4, pp 691-702.http://link.springer.com/article/10.1007%2Fs13105-015-0434-8ecsl@uevora.ptcarlasimoes3@hotmail.comlrodrigues-88@hotmail.comacrc@uevora.ptrvitorino@ua.ptfamado@ua.ptcmma@uevora.ptisabel.carmo72@gmail.com365DOI: 10.1007/s13105-015-0434-8Lamy, ElsaSimões, CarlaRodrigues, LéniaRodrigues Costa, AnaVitorino, RuiAmado, FranciscoAntunes, Céliado Carmo, Isabelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:05:49Zoai:dspace.uevora.pt:10174/18066Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:09:58.251731Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
title |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
spellingShingle |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not Lamy, Elsa saliva obesity protein profile |
title_short |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
title_full |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
title_fullStr |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
title_full_unstemmed |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
title_sort |
Changes in the salivary protein profile of morbidly obese women either previously subjected to bariatric surgery or not |
author |
Lamy, Elsa |
author_facet |
Lamy, Elsa Simões, Carla Rodrigues, Lénia Rodrigues Costa, Ana Vitorino, Rui Amado, Francisco Antunes, Célia do Carmo, Isabel |
author_role |
author |
author2 |
Simões, Carla Rodrigues, Lénia Rodrigues Costa, Ana Vitorino, Rui Amado, Francisco Antunes, Célia do Carmo, Isabel |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lamy, Elsa Simões, Carla Rodrigues, Lénia Rodrigues Costa, Ana Vitorino, Rui Amado, Francisco Antunes, Célia do Carmo, Isabel |
dc.subject.por.fl_str_mv |
saliva obesity protein profile |
topic |
saliva obesity protein profile |
description |
Saliva is a non-invasive source of biomarkers useful in the study of physiological mechanisms. Moreover, this fluid has diverse functions, among which food perception and ingestion, making it particularly suitable for the study of obesity. The aims of this study were to assess changes in salivary proteome among morbidly obese women, with a view to provide information about mechanisms potentially related to the development of obesity, and to evaluate whether these changes persist after weight loss. Mixed saliva samples from morbidly obese women (N = 18) who had been either subjected (group O-BS) or not (group O) to bariatric surgery and women with normal weight (N = 14; group C) were compared for protein profiles, alpha-amylase abundance and enzymatic activity, and carbonic anhydrase (CA) VI abundance. Differences in salivary obese profiles were observed for 23 different spots. Zinc-alpha-2 glycoprotein-containing spots showed higher abundance in group O only, whereas cystatin S-containing spots presented higher abundance in the two groups of obese subjects. Most of the spots identified as salivary amylase were present at lower levels in group O-BS. With regard to the amylase enzymatic activity, increases were observed for group O and decreases for group O-BS. One interesting finding was the high correlation between levels of CA VI and body mass index in group O, which was not observed for groups O-BS or C. The differences between groups, mainly regarding salivary proteins involved in taste sensitivity and metabolism, point to the potential of using saliva in the study of obesity development. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01T00:00:00Z 2016-03-15T12:24:24Z 2016-03-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/18066 http://hdl.handle.net/10174/18066 |
url |
http://hdl.handle.net/10174/18066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Elsa Lamy, Carla Simões, Lénia Rodrigues, Ana Rodrigues Costa, Rui Vitorino, Francisco Amado, Célia Antunes, Isabel do Carmo “Changes in salivary protein profile in morbid obese women with and without bariatric surgery", Journal of Physiology and Biochemistry”, December 2015, Volume 71, Issue 4, pp 691-702. http://link.springer.com/article/10.1007%2Fs13105-015-0434-8 ecsl@uevora.pt carlasimoes3@hotmail.com lrodrigues-88@hotmail.com acrc@uevora.pt rvitorino@ua.pt famado@ua.pt cmma@uevora.pt isabel.carmo72@gmail.com 365 DOI: 10.1007/s13105-015-0434-8 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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