Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants

Detalhes bibliográficos
Autor(a) principal: Nunes, Alexandra
Data de Publicação: 2023
Outros Autores: Oleastro, Mónica, Alves, Frederico, Liassine, Nadia, Lowe, David M., Benejat, Lucie, Ducounau, Astrid, Jehanne, Quentin, Borges, Vítor, Gomes, João Paulo, Godbole, Gauri, Philippe, Lehours
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8898
Resumo: Erratum in: Microbiol Spectr. 2023 Dec 12;11(6):e0312123. doi: 10.1128/spectrum.03121-23. Epub 2023 Oct 10.
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spelling Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance DeterminantsCampylobacter jejuniRecurrent InfectionHigh-level Carbapenem Resistanceimmunocompromised PatientsMacrolide ResistanceMajor PorinInfecções GastrointestinaisErratum in: Microbiol Spectr. 2023 Dec 12;11(6):e0312123. doi: 10.1128/spectrum.03121-23. Epub 2023 Oct 10.Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434052/We present two independent cases of recurrent multidrug-resistant Campylobacter jejuni infection in immunocompromised hosts and the clinical challenges encountered due to the development of high-level carbapenem resistance. The mechanisms associated with this unusual resistance for Campylobacters were characterized. Initial macrolide and carbapenem-susceptible strains acquired resistance to erythromycin (MIC . 256mg/L), ertapenem (MIC . 32mg/L), and meropenem (MIC . 32mg/L) during treatment. Carbapenem-resistant isolates developed an in-frame insertion resulting in an extra Asp residue in the major outer membrane protein PorA, within the extracellular loop L3 that connects b-strands 5 and 6 and forms a constriction zone involved in Ca21 binding. The isolates presenting the highest MIC to ertapenem exhibited an extra nonsynonymous mutation (G167AjGly56Asp) at PorA’s extracellular loop L1. IMPORTANCE Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.Importance: Carbapenem susceptibility patterns suggest drug impermeability, related to either insertion and/or single nucleotide polymorphism (SNP) within porA. Similar molecular events occurring in two independent cases support the association of these mechanisms with carbapenem resistance in Campylobacter spp.This work was partially supported by GenomePT (ref. POCI-01-0145-FEDER-022184) from Fundação para a Ciência e Tecnologia, Portugal.Repositório Científico do Instituto Nacional de SaúdeNunes, AlexandraOleastro, MónicaAlves, FredericoLiassine, NadiaLowe, David M.Benejat, LucieDucounau, AstridJehanne, QuentinBorges, VítorGomes, João PauloGodbole, GauriPhilippe, Lehours2024-01-12T16:23:51Z2023-08-172023-08-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8898engMicrobiol Spectr. 2023 Aug 17;11(4):e0107023. doi: 10.1128/spectrum.01070-23. Epub 2023 Jun 26.2165-049710.1128/spectrum.01070-23info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-20T01:31:50Zoai:repositorio.insa.pt:10400.18/8898Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:36:16.536488Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
title Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
spellingShingle Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
Nunes, Alexandra
Campylobacter jejuni
Recurrent Infection
High-level Carbapenem Resistance
immunocompromised Patients
Macrolide Resistance
Major Porin
Infecções Gastrointestinais
title_short Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
title_full Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
title_fullStr Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
title_full_unstemmed Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
title_sort Recurrent Campylobacter jejuni Infections with In Vivo Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants
author Nunes, Alexandra
author_facet Nunes, Alexandra
Oleastro, Mónica
Alves, Frederico
Liassine, Nadia
Lowe, David M.
Benejat, Lucie
Ducounau, Astrid
Jehanne, Quentin
Borges, Vítor
Gomes, João Paulo
Godbole, Gauri
Philippe, Lehours
author_role author
author2 Oleastro, Mónica
Alves, Frederico
Liassine, Nadia
Lowe, David M.
Benejat, Lucie
Ducounau, Astrid
Jehanne, Quentin
Borges, Vítor
Gomes, João Paulo
Godbole, Gauri
Philippe, Lehours
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Nunes, Alexandra
Oleastro, Mónica
Alves, Frederico
Liassine, Nadia
Lowe, David M.
Benejat, Lucie
Ducounau, Astrid
Jehanne, Quentin
Borges, Vítor
Gomes, João Paulo
Godbole, Gauri
Philippe, Lehours
dc.subject.por.fl_str_mv Campylobacter jejuni
Recurrent Infection
High-level Carbapenem Resistance
immunocompromised Patients
Macrolide Resistance
Major Porin
Infecções Gastrointestinais
topic Campylobacter jejuni
Recurrent Infection
High-level Carbapenem Resistance
immunocompromised Patients
Macrolide Resistance
Major Porin
Infecções Gastrointestinais
description Erratum in: Microbiol Spectr. 2023 Dec 12;11(6):e0312123. doi: 10.1128/spectrum.03121-23. Epub 2023 Oct 10.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-17
2023-08-17T00:00:00Z
2024-01-12T16:23:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8898
url http://hdl.handle.net/10400.18/8898
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbiol Spectr. 2023 Aug 17;11(4):e0107023. doi: 10.1128/spectrum.01070-23. Epub 2023 Jun 26.
2165-0497
10.1128/spectrum.01070-23
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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