Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures

Detalhes bibliográficos
Autor(a) principal: Dias, Marcelo
Data de Publicação: 2021
Outros Autores: Marques-Morgado, Inês, Coelho, Joana E, Ruivo, Pedro, Lopes, Luisa V., Remondes, Miguel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49220
Resumo: Copyright © 2021 Dias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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spelling Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structuresSuperior sagittal sinusSurgeryCopyright © 2021 Dias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.Stereotaxic access to brain areas underneath the superior sagittal sinus (SSS) is notoriously challenging. As a major drainage vessel, covering the whole extension of the sagittal fissure, the SSS impedes direct bilateral access to underlying regions for recording and stimulation probes, drug-delivery cannulas, and injection devices. We now describe a new method for transection and retraction of the SSS in rats, that allows the accurate placement of microinjection devices, or chronic electrode probes, while avoiding hemorrhage and the ensuing deleterious consequences for local structures, animal health, and behavior. To demonstrate the feasibility of this approach we evaluated its consequences acutely during surgery, and thereafter during surgical survival, recovery, behavioral testing, as well as postmortem analysis of histologic impact in the related brain structures of male rats. This method provides a new approach enabling direct access for manipulation and recording of activity in brain areas previously obstructed by the SSS.This work was supported by the Bial Foundation Grant 135/18; the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Grant PTDC/MED-NEU/29325/2017); Prémio Santa Casa-Prémio Mantero Belard (MB-7-2018); and FCT PhD Fellowships PD/PB/114125/2015 and PD/128395/2017, Scientific Employment Contract CEECIND/01497/2017, and Exploratory Grant IF/00201/2013; and an IMM Director’s Fund AwardeNeuroRepositório da Universidade de LisboaDias, MarceloMarques-Morgado, InêsCoelho, Joana ERuivo, PedroLopes, Luisa V.Remondes, Miguel2021-07-29T14:58:10Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49220engeNeuro. 2021 Jul 14;8(4):ENEURO.0146-21.202110.1523/ENEURO.0146-21.20212373-2822info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:47Zoai:repositorio.ul.pt:10451/49220Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:52.099677Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
title Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
spellingShingle Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
Dias, Marcelo
Superior sagittal sinus
Surgery
title_short Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
title_full Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
title_fullStr Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
title_full_unstemmed Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
title_sort Transection of the superior sagittal sinus enables bilateral access to the rodent midline brain structures
author Dias, Marcelo
author_facet Dias, Marcelo
Marques-Morgado, Inês
Coelho, Joana E
Ruivo, Pedro
Lopes, Luisa V.
Remondes, Miguel
author_role author
author2 Marques-Morgado, Inês
Coelho, Joana E
Ruivo, Pedro
Lopes, Luisa V.
Remondes, Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Dias, Marcelo
Marques-Morgado, Inês
Coelho, Joana E
Ruivo, Pedro
Lopes, Luisa V.
Remondes, Miguel
dc.subject.por.fl_str_mv Superior sagittal sinus
Surgery
topic Superior sagittal sinus
Surgery
description Copyright © 2021 Dias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-29T14:58:10Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49220
url http://hdl.handle.net/10451/49220
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv eNeuro. 2021 Jul 14;8(4):ENEURO.0146-21.2021
10.1523/ENEURO.0146-21.2021
2373-2822
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv eNeuro
publisher.none.fl_str_mv eNeuro
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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