Chronic arthritis leads to disturbances in the bone collagen network
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/6012 |
Resumo: | © 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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Chronic arthritis leads to disturbances in the bone collagen networkChronic arthritisBone collagen network© 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Introduction: In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by chronic inflammation is associated with an imbalance in bone turnover and also a disorganization of the bone type I collagen network. Methods: Serum, vertebrae and femur bones were collected from eight-month-old polyarthritis SKG mice and controls. Strength of the femoral bones was evaluated using three-point bending tests and density was assessed with a pycnometer. Bone turnover markers carboxy-terminal collagen cross-linking telopeptides (CTX-I) and amino-terminal propeptide of type I procollagen (PINP) were measured in serum. The organization and density of bone collagen were analyzed in vertebrae using second-harmonic generation (SHG) imaging with a two-photon microscope and trabecular bone microstructure was assessed by scanning electron microscope (SEM). Results: Femoral bones of SKG mice revealed increased fragility expressed by deterioration of mechanical properties, namely altered stiffness (P = 0.007) and reduced strength (P = 0.006), when compared to controls. Accordingly, intertrabecular distance and trabecular thickness as observed by SEM were reduced in SKG mice. PINP was significantly higher in arthritic mice (9.18 ± 3.21 ng/ml) when compared to controls (1.71 ± 0.53 ng/ml, P < 0.001). Bone resorption marker CTX-I was 9.67 ± 3.18 ng/ml in arthritic SKG mice compared to 6.23 ± 4.11 ng/ml in controls (P = 0.176). The forward-to-backward signal ratio measured by SHG was higher in SKG animals, reflecting disorganized matrix and loose collagen structure, compared to controls. Conclusions: We have shown for the first time that chronic arthritis by itself impairs bone matrix architecture, probably due to disturbed bone remodeling and increased collagen turnover. This effect might predispose patients to bone fragility fractures.This work was supported by the PTDC/SAU-BEB/65992/2006 grant from Fundação para a Ciência e Tecnologia.BioMed CentralRepositório da Universidade de LisboaCaetano-Lopes, JoanaNery, Ana M.Canhao, HelenaDuarte, JoanaCascão, RitaRodrigues, AnaPerpétuo, Inês P.Abdulghani, SabaAmaral, Pedro M.Sakaguchi, ShimonKonttinen, Yrjö T.Graça, LuísVaz, Maria F.Fonseca, João Eurico2012-04-17T14:11:21Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6012engArthritis Research & Therapy 2010, 12:R91478-6354info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:48:10Zoai:repositorio.ul.pt:10451/6012Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:13.372142Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Chronic arthritis leads to disturbances in the bone collagen network |
| title |
Chronic arthritis leads to disturbances in the bone collagen network |
| spellingShingle |
Chronic arthritis leads to disturbances in the bone collagen network Caetano-Lopes, Joana Chronic arthritis Bone collagen network |
| title_short |
Chronic arthritis leads to disturbances in the bone collagen network |
| title_full |
Chronic arthritis leads to disturbances in the bone collagen network |
| title_fullStr |
Chronic arthritis leads to disturbances in the bone collagen network |
| title_full_unstemmed |
Chronic arthritis leads to disturbances in the bone collagen network |
| title_sort |
Chronic arthritis leads to disturbances in the bone collagen network |
| author |
Caetano-Lopes, Joana |
| author_facet |
Caetano-Lopes, Joana Nery, Ana M. Canhao, Helena Duarte, Joana Cascão, Rita Rodrigues, Ana Perpétuo, Inês P. Abdulghani, Saba Amaral, Pedro M. Sakaguchi, Shimon Konttinen, Yrjö T. Graça, Luís Vaz, Maria F. Fonseca, João Eurico |
| author_role |
author |
| author2 |
Nery, Ana M. Canhao, Helena Duarte, Joana Cascão, Rita Rodrigues, Ana Perpétuo, Inês P. Abdulghani, Saba Amaral, Pedro M. Sakaguchi, Shimon Konttinen, Yrjö T. Graça, Luís Vaz, Maria F. Fonseca, João Eurico |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Caetano-Lopes, Joana Nery, Ana M. Canhao, Helena Duarte, Joana Cascão, Rita Rodrigues, Ana Perpétuo, Inês P. Abdulghani, Saba Amaral, Pedro M. Sakaguchi, Shimon Konttinen, Yrjö T. Graça, Luís Vaz, Maria F. Fonseca, João Eurico |
| dc.subject.por.fl_str_mv |
Chronic arthritis Bone collagen network |
| topic |
Chronic arthritis Bone collagen network |
| description |
© 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z 2012-04-17T14:11:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/6012 |
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http://hdl.handle.net/10451/6012 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Arthritis Research & Therapy 2010, 12:R9 1478-6354 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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BioMed Central |
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BioMed Central |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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