Chronic arthritis leads to disturbances in the bone collagen network

Detalhes bibliográficos
Autor(a) principal: Caetano-Lopes, Joana
Data de Publicação: 2010
Outros Autores: Nery, Ana M., Canhao, Helena, Duarte, Joana, Cascão, Rita, Rodrigues, Ana, Perpétuo, Inês P., Abdulghani, Saba, Amaral, Pedro M., Sakaguchi, Shimon, Konttinen, Yrjö T., Graça, Luís, Vaz, Maria F., Fonseca, João Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/6012
Resumo: © 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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spelling Chronic arthritis leads to disturbances in the bone collagen networkChronic arthritisBone collagen network© 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Introduction: In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by chronic inflammation is associated with an imbalance in bone turnover and also a disorganization of the bone type I collagen network. Methods: Serum, vertebrae and femur bones were collected from eight-month-old polyarthritis SKG mice and controls. Strength of the femoral bones was evaluated using three-point bending tests and density was assessed with a pycnometer. Bone turnover markers carboxy-terminal collagen cross-linking telopeptides (CTX-I) and amino-terminal propeptide of type I procollagen (PINP) were measured in serum. The organization and density of bone collagen were analyzed in vertebrae using second-harmonic generation (SHG) imaging with a two-photon microscope and trabecular bone microstructure was assessed by scanning electron microscope (SEM). Results: Femoral bones of SKG mice revealed increased fragility expressed by deterioration of mechanical properties, namely altered stiffness (P = 0.007) and reduced strength (P = 0.006), when compared to controls. Accordingly, intertrabecular distance and trabecular thickness as observed by SEM were reduced in SKG mice. PINP was significantly higher in arthritic mice (9.18 ± 3.21 ng/ml) when compared to controls (1.71 ± 0.53 ng/ml, P < 0.001). Bone resorption marker CTX-I was 9.67 ± 3.18 ng/ml in arthritic SKG mice compared to 6.23 ± 4.11 ng/ml in controls (P = 0.176). The forward-to-backward signal ratio measured by SHG was higher in SKG animals, reflecting disorganized matrix and loose collagen structure, compared to controls. Conclusions: We have shown for the first time that chronic arthritis by itself impairs bone matrix architecture, probably due to disturbed bone remodeling and increased collagen turnover. This effect might predispose patients to bone fragility fractures.This work was supported by the PTDC/SAU-BEB/65992/2006 grant from Fundação para a Ciência e Tecnologia.BioMed CentralRepositório da Universidade de LisboaCaetano-Lopes, JoanaNery, Ana M.Canhao, HelenaDuarte, JoanaCascão, RitaRodrigues, AnaPerpétuo, Inês P.Abdulghani, SabaAmaral, Pedro M.Sakaguchi, ShimonKonttinen, Yrjö T.Graça, LuísVaz, Maria F.Fonseca, João Eurico2012-04-17T14:11:21Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6012engArthritis Research & Therapy 2010, 12:R91478-6354info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:48:10Zoai:repositorio.ul.pt:10451/6012Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:13.372142Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chronic arthritis leads to disturbances in the bone collagen network
title Chronic arthritis leads to disturbances in the bone collagen network
spellingShingle Chronic arthritis leads to disturbances in the bone collagen network
Caetano-Lopes, Joana
Chronic arthritis
Bone collagen network
title_short Chronic arthritis leads to disturbances in the bone collagen network
title_full Chronic arthritis leads to disturbances in the bone collagen network
title_fullStr Chronic arthritis leads to disturbances in the bone collagen network
title_full_unstemmed Chronic arthritis leads to disturbances in the bone collagen network
title_sort Chronic arthritis leads to disturbances in the bone collagen network
author Caetano-Lopes, Joana
author_facet Caetano-Lopes, Joana
Nery, Ana M.
Canhao, Helena
Duarte, Joana
Cascão, Rita
Rodrigues, Ana
Perpétuo, Inês P.
Abdulghani, Saba
Amaral, Pedro M.
Sakaguchi, Shimon
Konttinen, Yrjö T.
Graça, Luís
Vaz, Maria F.
Fonseca, João Eurico
author_role author
author2 Nery, Ana M.
Canhao, Helena
Duarte, Joana
Cascão, Rita
Rodrigues, Ana
Perpétuo, Inês P.
Abdulghani, Saba
Amaral, Pedro M.
Sakaguchi, Shimon
Konttinen, Yrjö T.
Graça, Luís
Vaz, Maria F.
Fonseca, João Eurico
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Caetano-Lopes, Joana
Nery, Ana M.
Canhao, Helena
Duarte, Joana
Cascão, Rita
Rodrigues, Ana
Perpétuo, Inês P.
Abdulghani, Saba
Amaral, Pedro M.
Sakaguchi, Shimon
Konttinen, Yrjö T.
Graça, Luís
Vaz, Maria F.
Fonseca, João Eurico
dc.subject.por.fl_str_mv Chronic arthritis
Bone collagen network
topic Chronic arthritis
Bone collagen network
description © 2010 Caetano-Lopes et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2012-04-17T14:11:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6012
url http://hdl.handle.net/10451/6012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arthritis Research & Therapy 2010, 12:R9
1478-6354
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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