Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco

Detalhes bibliográficos
Autor(a) principal: Lbida, B.
Data de Publicação: 2004
Outros Autores: Fonseca, Filomena, Santos, C., Zemzami, M., Bennani, A., Nolasco, Gustavo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/6247
Resumo: Genomic variability of the coat protein gene of Citrus tristeza virus isolates obtained from old Meyer lemon introductions in Morocco and more recent budwood introductions from Spain were studied. The coat protein gene of the virus was amplified directly from infected tissue by immunocapture RT-PCR and analysed by single stranded conformation polymorphism (SSCP) and sequencing. Each isolate consisted of several related genomic variants, typical of a quasi-species. Although SSCP analysis has only limited typing ability it could be used in an initial screening to discriminate between isolates of different origin and to analyse the genomic structure of each isolate. Sequence analysis showed that the isolates of Spanish origin were closely related to mild isolates characterised in Florida and in Portugal. The Meyer lemon isolate on the other hand was related to severe strains of Meyer lemon characterised in Florida some years ago and to other severe strains from Brasil. A knowledge of the coat protein gene sequence is useful to trace the origin of the isolates.
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spelling Genomic variability of Citrus tristeza virus (CTV) isolates introduced into MoroccoGenomic variability of the coat protein gene of Citrus tristeza virus isolates obtained from old Meyer lemon introductions in Morocco and more recent budwood introductions from Spain were studied. The coat protein gene of the virus was amplified directly from infected tissue by immunocapture RT-PCR and analysed by single stranded conformation polymorphism (SSCP) and sequencing. Each isolate consisted of several related genomic variants, typical of a quasi-species. Although SSCP analysis has only limited typing ability it could be used in an initial screening to discriminate between isolates of different origin and to analyse the genomic structure of each isolate. Sequence analysis showed that the isolates of Spanish origin were closely related to mild isolates characterised in Florida and in Portugal. The Meyer lemon isolate on the other hand was related to severe strains of Meyer lemon characterised in Florida some years ago and to other severe strains from Brasil. A knowledge of the coat protein gene sequence is useful to trace the origin of the isolates.SapientiaLbida, B.Fonseca, FilomenaSantos, C.Zemzami, M.Bennani, A.Nolasco, Gustavo2015-06-15T14:13:48Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/6247eng0031-9465AUT: FFO00315; GNO00324;info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:17:27Zoai:sapientia.ualg.pt:10400.1/6247Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:59:03.247328Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
title Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
spellingShingle Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
Lbida, B.
title_short Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
title_full Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
title_fullStr Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
title_full_unstemmed Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
title_sort Genomic variability of Citrus tristeza virus (CTV) isolates introduced into Morocco
author Lbida, B.
author_facet Lbida, B.
Fonseca, Filomena
Santos, C.
Zemzami, M.
Bennani, A.
Nolasco, Gustavo
author_role author
author2 Fonseca, Filomena
Santos, C.
Zemzami, M.
Bennani, A.
Nolasco, Gustavo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Lbida, B.
Fonseca, Filomena
Santos, C.
Zemzami, M.
Bennani, A.
Nolasco, Gustavo
description Genomic variability of the coat protein gene of Citrus tristeza virus isolates obtained from old Meyer lemon introductions in Morocco and more recent budwood introductions from Spain were studied. The coat protein gene of the virus was amplified directly from infected tissue by immunocapture RT-PCR and analysed by single stranded conformation polymorphism (SSCP) and sequencing. Each isolate consisted of several related genomic variants, typical of a quasi-species. Although SSCP analysis has only limited typing ability it could be used in an initial screening to discriminate between isolates of different origin and to analyse the genomic structure of each isolate. Sequence analysis showed that the isolates of Spanish origin were closely related to mild isolates characterised in Florida and in Portugal. The Meyer lemon isolate on the other hand was related to severe strains of Meyer lemon characterised in Florida some years ago and to other severe strains from Brasil. A knowledge of the coat protein gene sequence is useful to trace the origin of the isolates.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2015-06-15T14:13:48Z
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dc.language.iso.fl_str_mv eng
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