Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/83186 |
Resumo: | he combination of two or more agents capable of acting in synergy has been reported as a valuable tool to fight against pathogens. Silver nanoparticles (AgNPs) present a strong antimicrobial action, although their cytotoxicity for healthy cells at active concentrations is a major concern. Azoimidazole moieties exhibit interesting bioactivities, including antimicrobial activity. In this work, a class of recently described azoimidazoles with strong antifungal activity was conjugated with citrate or polyvinylpyrrolidone-stabilized AgNPs. Proton nuclear magnetic resonance was used to confirm the purity of the compounds before further tests and atomic absorption spectroscopy to verify the concentration of silver in the prepared dispersions. Other analytical techniques elucidate the morphology and stability of AgNPs and corresponding conjugates, namely ultraviolet–visible spectrophotometry, scanning transmission electron microscopy and dynamic light scattering analysis. The synergistic antimicrobial activity of the conjugates was assessed through a checkerboard assay against yeasts (Candida albicans and Candida krusei) and bacteria (Staphylococcus aureus and Escherichia coli). The conjugates showed improved antimicrobial activity against all microorganisms, in particular towards bacteria, with concentrations below their individual minimal inhibitory concentration (MIC). Furthermore, some combinations were found to be non-cytotoxic towards human HaCaT cells. |
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Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazolesAntifungalAntibacterialSynergismSilver nanoparticlesStabilizing agentsCiências Naturais::Ciências QuímicasEngenharia e Tecnologia::Engenharia dos MateriaisScience & Technologyhe combination of two or more agents capable of acting in synergy has been reported as a valuable tool to fight against pathogens. Silver nanoparticles (AgNPs) present a strong antimicrobial action, although their cytotoxicity for healthy cells at active concentrations is a major concern. Azoimidazole moieties exhibit interesting bioactivities, including antimicrobial activity. In this work, a class of recently described azoimidazoles with strong antifungal activity was conjugated with citrate or polyvinylpyrrolidone-stabilized AgNPs. Proton nuclear magnetic resonance was used to confirm the purity of the compounds before further tests and atomic absorption spectroscopy to verify the concentration of silver in the prepared dispersions. Other analytical techniques elucidate the morphology and stability of AgNPs and corresponding conjugates, namely ultraviolet–visible spectrophotometry, scanning transmission electron microscopy and dynamic light scattering analysis. The synergistic antimicrobial activity of the conjugates was assessed through a checkerboard assay against yeasts (Candida albicans and Candida krusei) and bacteria (Staphylococcus aureus and Escherichia coli). The conjugates showed improved antimicrobial activity against all microorganisms, in particular towards bacteria, with concentrations below their individual minimal inhibitory concentration (MIC). Furthermore, some combinations were found to be non-cytotoxic towards human HaCaT cells.This work was funded by the European Regional Development Fund through the Oper ational Competitiveness Program and through the National Foundation for Science and Technol ogy of Portugal (FCT) under the projects UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), UIDB/00686/2020 of the Chemistry Centre of University of Minho (CQUM), UIBD/04423/2020 and UIDP/04423/2020 of the CIIMAR/CIMAR, Interdisciplinary Centre of Ma rine and Environmental Research and FCT Project MEDCOR PTDC/CTM-TEX/1213/2020. Ana Isabel Ribeiro acknowledges FCT for the funding of her Ph.D. scholarship SFRH/BD/137668/2018.MDPIUniversidade do MinhoRibeiro, Ana Isabel FerreiraVieira, Bárbara MarquesDantas, Daniela Filipa CostaSilva, BárbaraPinto, EugéniaCerqueira, FátimaSilva, RenataFernando RemiãoPadrão, JorgeDias, AliceZille, Andrea20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83186engRibeiro, A. I., Vieira, B., Dantas, D., Silva, B., Pinto, E., Cerqueira, F., … Zille, A. (2023, March 13). Synergistic Antimicrobial Activity of Silver Nanoparticles with an Emergent Class of Azoimidazoles. Pharmaceutics. MDPI AG. http://doi.org/10.3390/pharmaceutics150309261999-492310.3390/pharmaceutics15030926https://www.mdpi.com/1999-4923/15/3/926info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:25Zoai:repositorium.sdum.uminho.pt:1822/83186Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:23.666246Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
title |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
spellingShingle |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles Ribeiro, Ana Isabel Ferreira Antifungal Antibacterial Synergism Silver nanoparticles Stabilizing agents Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
title_short |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
title_full |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
title_fullStr |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
title_full_unstemmed |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
title_sort |
Synergistic antimicrobial activity of silver nanoparticles with an emergent class of azoimidazoles |
author |
Ribeiro, Ana Isabel Ferreira |
author_facet |
Ribeiro, Ana Isabel Ferreira Vieira, Bárbara Marques Dantas, Daniela Filipa Costa Silva, Bárbara Pinto, Eugénia Cerqueira, Fátima Silva, Renata Fernando Remião Padrão, Jorge Dias, Alice Zille, Andrea |
author_role |
author |
author2 |
Vieira, Bárbara Marques Dantas, Daniela Filipa Costa Silva, Bárbara Pinto, Eugénia Cerqueira, Fátima Silva, Renata Fernando Remião Padrão, Jorge Dias, Alice Zille, Andrea |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Ana Isabel Ferreira Vieira, Bárbara Marques Dantas, Daniela Filipa Costa Silva, Bárbara Pinto, Eugénia Cerqueira, Fátima Silva, Renata Fernando Remião Padrão, Jorge Dias, Alice Zille, Andrea |
dc.subject.por.fl_str_mv |
Antifungal Antibacterial Synergism Silver nanoparticles Stabilizing agents Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
topic |
Antifungal Antibacterial Synergism Silver nanoparticles Stabilizing agents Ciências Naturais::Ciências Químicas Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
description |
he combination of two or more agents capable of acting in synergy has been reported as a valuable tool to fight against pathogens. Silver nanoparticles (AgNPs) present a strong antimicrobial action, although their cytotoxicity for healthy cells at active concentrations is a major concern. Azoimidazole moieties exhibit interesting bioactivities, including antimicrobial activity. In this work, a class of recently described azoimidazoles with strong antifungal activity was conjugated with citrate or polyvinylpyrrolidone-stabilized AgNPs. Proton nuclear magnetic resonance was used to confirm the purity of the compounds before further tests and atomic absorption spectroscopy to verify the concentration of silver in the prepared dispersions. Other analytical techniques elucidate the morphology and stability of AgNPs and corresponding conjugates, namely ultraviolet–visible spectrophotometry, scanning transmission electron microscopy and dynamic light scattering analysis. The synergistic antimicrobial activity of the conjugates was assessed through a checkerboard assay against yeasts (Candida albicans and Candida krusei) and bacteria (Staphylococcus aureus and Escherichia coli). The conjugates showed improved antimicrobial activity against all microorganisms, in particular towards bacteria, with concentrations below their individual minimal inhibitory concentration (MIC). Furthermore, some combinations were found to be non-cytotoxic towards human HaCaT cells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/83186 |
url |
https://hdl.handle.net/1822/83186 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, A. I., Vieira, B., Dantas, D., Silva, B., Pinto, E., Cerqueira, F., … Zille, A. (2023, March 13). Synergistic Antimicrobial Activity of Silver Nanoparticles with an Emergent Class of Azoimidazoles. Pharmaceutics. MDPI AG. http://doi.org/10.3390/pharmaceutics15030926 1999-4923 10.3390/pharmaceutics15030926 https://www.mdpi.com/1999-4923/15/3/926 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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