Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3181 |
Resumo: | The changes in the regulation of at mitochondrial NADP-isocitrate dehydrogenase (NADP-ICDH) in a rat heart during have been analysed. Increase of enzyme activity in the cytosol and mitochondria of the heart ischemia was detected. Catalytic properties of the mitochondrial NADP-ICDH at norm and pathology have been compared on homogeneous enzyme preparations. Enzyme from the normoxic and ischemic heart showed the same electrophoretical mobility and molecular mass. Enzyme isolated from the ischemic heart mitochondria demonstrated higher activation energy and lower thermal stability. NADP-isocitrate dehydrogenase at the normoxic and ischemic conditions exhibited different Km for substrates and regulatory behaviour in relation to ATP, ADP, 2-oxoglutarate, citrate, malate, reduced and oxidised glutathione. The inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals was lower in the ischemic enzyme. We hypothesise that the specific features of regulation behaviour of NADP-ICDH from the ischemic tissues permits the enzyme to supply NADPH to the glutathione reductase/glutathione peroxidase system. |
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Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemiaIschemiaOxidative stressNADP-isocitrate dehydrogenaseEnzyme regulation.Faculdade de Ciências da VidaThe changes in the regulation of at mitochondrial NADP-isocitrate dehydrogenase (NADP-ICDH) in a rat heart during have been analysed. Increase of enzyme activity in the cytosol and mitochondria of the heart ischemia was detected. Catalytic properties of the mitochondrial NADP-ICDH at norm and pathology have been compared on homogeneous enzyme preparations. Enzyme from the normoxic and ischemic heart showed the same electrophoretical mobility and molecular mass. Enzyme isolated from the ischemic heart mitochondria demonstrated higher activation energy and lower thermal stability. NADP-isocitrate dehydrogenase at the normoxic and ischemic conditions exhibited different Km for substrates and regulatory behaviour in relation to ATP, ADP, 2-oxoglutarate, citrate, malate, reduced and oxidised glutathione. The inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals was lower in the ischemic enzyme. We hypothesise that the specific features of regulation behaviour of NADP-ICDH from the ischemic tissues permits the enzyme to supply NADPH to the glutathione reductase/glutathione peroxidase system.This work was supported by grants from the Scientific Programs of Russia Ministry Education and Science (number of projects are RNP. 2.1.1.4429 and UR. 07.01.004).SpringerDigitUMaPopova, TatianaCarvalho, Miguel Â. A. Pinheiro deMatasova, LarisaMedvedeva, Liliya2021-03-18T14:27:47Z2006-01-01T00:00:00Z2006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3181engPopova, T., Carvalho, M. A. P., Matasova, L., & Medvedeva, L. (2007). Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia. Molecular and cellular biochemistry, 294(1), 97-105.DOI: 10.1007/s11010-006-9249-910.1007/s11010-006-9249-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:56:06ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
title |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
spellingShingle |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia Popova, Tatiana Ischemia Oxidative stress NADP-isocitrate dehydrogenase Enzyme regulation . Faculdade de Ciências da Vida |
title_short |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
title_full |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
title_fullStr |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
title_full_unstemmed |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
title_sort |
Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia |
author |
Popova, Tatiana |
author_facet |
Popova, Tatiana Carvalho, Miguel Â. A. Pinheiro de Matasova, Larisa Medvedeva, Liliya |
author_role |
author |
author2 |
Carvalho, Miguel Â. A. Pinheiro de Matasova, Larisa Medvedeva, Liliya |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Popova, Tatiana Carvalho, Miguel Â. A. Pinheiro de Matasova, Larisa Medvedeva, Liliya |
dc.subject.por.fl_str_mv |
Ischemia Oxidative stress NADP-isocitrate dehydrogenase Enzyme regulation . Faculdade de Ciências da Vida |
topic |
Ischemia Oxidative stress NADP-isocitrate dehydrogenase Enzyme regulation . Faculdade de Ciências da Vida |
description |
The changes in the regulation of at mitochondrial NADP-isocitrate dehydrogenase (NADP-ICDH) in a rat heart during have been analysed. Increase of enzyme activity in the cytosol and mitochondria of the heart ischemia was detected. Catalytic properties of the mitochondrial NADP-ICDH at norm and pathology have been compared on homogeneous enzyme preparations. Enzyme from the normoxic and ischemic heart showed the same electrophoretical mobility and molecular mass. Enzyme isolated from the ischemic heart mitochondria demonstrated higher activation energy and lower thermal stability. NADP-isocitrate dehydrogenase at the normoxic and ischemic conditions exhibited different Km for substrates and regulatory behaviour in relation to ATP, ADP, 2-oxoglutarate, citrate, malate, reduced and oxidised glutathione. The inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals was lower in the ischemic enzyme. We hypothesise that the specific features of regulation behaviour of NADP-ICDH from the ischemic tissues permits the enzyme to supply NADPH to the glutathione reductase/glutathione peroxidase system. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01T00:00:00Z 2006-01-01T00:00:00Z 2021-03-18T14:27:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3181 |
url |
http://hdl.handle.net/10400.13/3181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Popova, T., Carvalho, M. A. P., Matasova, L., & Medvedeva, L. (2007). Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia. Molecular and cellular biochemistry, 294(1), 97-105.DOI: 10.1007/s11010-006-9249-9 10.1007/s11010-006-9249-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777301213344169984 |