Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate

Detalhes bibliográficos
Autor(a) principal: Carvalho, Rui A.
Data de Publicação: 1999
Outros Autores: Babcock, Evelyn E., Jeffrey, F. Mark H., Sherry, A. Dean, Malloy, Craig R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
DOI: 10.1002/(SICI)1522-2594(199907)42:1
Texto Completo: http://hdl.handle.net/10316/8105
https://doi.org/10.1002/(SICI)1522-2594(199907)42:1<197
Resumo: Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc.
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spelling Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamateMost 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc.1999info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8105http://hdl.handle.net/10316/8105https://doi.org/10.1002/(SICI)1522-2594(199907)42:1<197engMagnetic Resonance in Medicine. 42:1 (1999) 197-200Carvalho, Rui A.Babcock, Evelyn E.Jeffrey, F. Mark H.Sherry, A. DeanMalloy, Craig R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T13:39:35Zoai:estudogeral.uc.pt:10316/8105Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:44.542752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
title Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
spellingShingle Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
Carvalho, Rui A.
Carvalho, Rui A.
title_short Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
title_full Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
title_fullStr Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
title_full_unstemmed Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
title_sort Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
author Carvalho, Rui A.
author_facet Carvalho, Rui A.
Carvalho, Rui A.
Babcock, Evelyn E.
Jeffrey, F. Mark H.
Sherry, A. Dean
Malloy, Craig R.
Babcock, Evelyn E.
Jeffrey, F. Mark H.
Sherry, A. Dean
Malloy, Craig R.
author_role author
author2 Babcock, Evelyn E.
Jeffrey, F. Mark H.
Sherry, A. Dean
Malloy, Craig R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Rui A.
Babcock, Evelyn E.
Jeffrey, F. Mark H.
Sherry, A. Dean
Malloy, Craig R.
description Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/8105
http://hdl.handle.net/10316/8105
https://doi.org/10.1002/(SICI)1522-2594(199907)42:1<197
url http://hdl.handle.net/10316/8105
https://doi.org/10.1002/(SICI)1522-2594(199907)42:1<197
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Magnetic Resonance in Medicine. 42:1 (1999) 197-200
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.identifier.doi.none.fl_str_mv 10.1002/(SICI)1522-2594(199907)42:1

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