Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production

• Autor(a) principal: Faustino, Lucas
• Data de Publicação: 2012
• Outros Autores: Mucida, Daniel, Keller, Alexandre Castro, Demengeot, Jocelyne, Bortoluci, Karina, Sardinha, Luiz Roberto, Carla Takenaka, Maisa, Basso, Alexandre Salgado, Faria, Ana Maria Caetano, Russo, Momtchilo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.7/637
• topic: Animals; Antigens, CD4; Asthma; Cytokines; Female; Interleukin-2 Receptor alpha Subunit; Lung; Mice; Mice, Inbred BALB C; Pneumonia; T-Lymphocytes, Regulatory; Th2 Cells; Cell Proliferation

Foxp3(+)CD25(+)CD4(+) regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To induce lung allergic inflammation, mice were sensitized twice with ovalbumin/aluminum hydroxide gel and challenged twice with intranasal ovalbumin. Tolerance was induced by oral administration of ovalbumin for 5 consecutive days prior to OVA sensitization and challenge. We detected regulatory T cells (Foxp3(+)CD25(+)CD4(+) T cells) in the airways of allergic and tolerant mice; however, the number of regulatory T cells was more than 40-fold higher in allergic mice than in tolerant mice. Lung regulatory T cells expressed an effector/memory phenotype (CCR4(high)CD62L(low)CD44(high)CD54(high)CD69(+)) that distinguished them from naive regulatory T cells (CCR4(int)CD62L(high)CD44(int)CD54(int)CD69(-)). These regulatory T cells efficiently suppressed pulmonary T-cell proliferation but not Th2 cytokine production.