DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response

Detalhes bibliográficos
Autor(a) principal: Camacho, Pedro
Data de Publicação: 2023
Outros Autores: Ribeiro, Edna, Pereira, Bruno, Varandas, Teresa, Nascimento, João, Henriques, José, Dutra-Medeiros, Marco, Delgadinho, Mariana, Oliveira, Ketlyn, Silva, Carina, Brito, Miguel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/15938
Resumo: This project was partially supported by an IDI&CA grant IPL/2021/DiffMeDiME_ESTeSL by H&TRC - Health & Technology Research Center, ESTeSL, Saúde, Instituto Politécnico de Lisboa and by Retina Institute of Lisbon (IRL).
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spelling DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema responseDNA methylationDiabetic retinopathyDiabetes mellitusEpigeneticsMacular edemaIPL/2021/DiffMeDiME_ESTeSLThis project was partially supported by an IDI&CA grant IPL/2021/DiffMeDiME_ESTeSL by H&TRC - Health & Technology Research Center, ESTeSL, Saúde, Instituto Politécnico de Lisboa and by Retina Institute of Lisbon (IRL).Purpose: DNA methylation is involved in Diabetic Retinopathy progression showing a metabolic memory mechanism. However, the association of DNA methyltransferase with diabetic macular edema is still unknown. We aimed to describe the differences in DNA methyltransferase gene expression in patients with different diabetic macular edema responses. Methods: A total of 27 diabetic patients, aged 59-90 years, were prospectively enrolled in this cross-sectional study. The participants were classified into the control group (CG, n = 11), diabetic macular edema responders (rDME, n = 9), and non-responder diabetic macular edema (nrDME, n = 7) after anti-vascular endothelial growth factor (anti-VEGF) treatment. Only cases with a complete ophthalmological examination, digital 133° color fundus, and SD-OCT assessments were used. After RNA extraction and first-strand cDNA synthesis, quantitative real-time PCR was performed with specific primers on the CFX Connect™ Real-Time PCR Detection System to assess differential transcriptional expression patterns. Results: The DNMT1 gene showed a positive correlation (r = 0.617; p = 0.043) with Best Corrected Visual Acuity (BCVA) in CG, a positive correlation (r = 0.917; p = 0.010) with HbA1c in nrDME and a negative correlation (r = -0.659; p = 0.049) with GCL-IPL thickness in rDME. DNMT3A gene showed a positive correlation (r = -0.890; p = 0.001) with Sub-foveal Choroidal thickness in rDME whereas DNMT3b gene showed a negative correlation (r = -0.815; p = 0.007) with HbA1c and RNFL (r = -0.664; p = 0.026) in CG. Conclusions: Patients with similar metabolic profile risk factors showed associated DNA methyltransferase transcriptional expression patterns differences fitting with the anti-VEGF diabetic macular edema response. Further studies are needed to clarify if these results (1) reflect disease evolution, (2) translate the therapeutic impact, (3) or can help to predict the therapeutic resistance profile.SageRCIPLCamacho, PedroRibeiro, EdnaPereira, BrunoVarandas, TeresaNascimento, JoãoHenriques, JoséDutra-Medeiros, MarcoDelgadinho, MarianaOliveira, KetlynSilva, CarinaBrito, Miguel2023-04-26T10:34:45Z2023-042023-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/15938engCamacho P, Ribeiro E, Delgadinho M, Oliveira K, Silva C, Brito M, et al. DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response. Eur J Ophthalmol. 2023;33(6):2267-74.10.1177/11206721231171623info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:16:00Zoai:repositorio.ipl.pt:10400.21/15938Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:23:31.482447Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
title DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
spellingShingle DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
Camacho, Pedro
DNA methylation
Diabetic retinopathy
Diabetes mellitus
Epigenetics
Macular edema
IPL/2021/DiffMeDiME_ESTeSL
title_short DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
title_full DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
title_fullStr DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
title_full_unstemmed DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
title_sort DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response
author Camacho, Pedro
author_facet Camacho, Pedro
Ribeiro, Edna
Pereira, Bruno
Varandas, Teresa
Nascimento, João
Henriques, José
Dutra-Medeiros, Marco
Delgadinho, Mariana
Oliveira, Ketlyn
Silva, Carina
Brito, Miguel
author_role author
author2 Ribeiro, Edna
Pereira, Bruno
Varandas, Teresa
Nascimento, João
Henriques, José
Dutra-Medeiros, Marco
Delgadinho, Mariana
Oliveira, Ketlyn
Silva, Carina
Brito, Miguel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Camacho, Pedro
Ribeiro, Edna
Pereira, Bruno
Varandas, Teresa
Nascimento, João
Henriques, José
Dutra-Medeiros, Marco
Delgadinho, Mariana
Oliveira, Ketlyn
Silva, Carina
Brito, Miguel
dc.subject.por.fl_str_mv DNA methylation
Diabetic retinopathy
Diabetes mellitus
Epigenetics
Macular edema
IPL/2021/DiffMeDiME_ESTeSL
topic DNA methylation
Diabetic retinopathy
Diabetes mellitus
Epigenetics
Macular edema
IPL/2021/DiffMeDiME_ESTeSL
description This project was partially supported by an IDI&CA grant IPL/2021/DiffMeDiME_ESTeSL by H&TRC - Health & Technology Research Center, ESTeSL, Saúde, Instituto Politécnico de Lisboa and by Retina Institute of Lisbon (IRL).
publishDate 2023
dc.date.none.fl_str_mv 2023-04-26T10:34:45Z
2023-04
2023-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/15938
url http://hdl.handle.net/10400.21/15938
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Camacho P, Ribeiro E, Delgadinho M, Oliveira K, Silva C, Brito M, et al. DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response. Eur J Ophthalmol. 2023;33(6):2267-74.
10.1177/11206721231171623
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage
publisher.none.fl_str_mv Sage
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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