On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal

Detalhes bibliográficos
Autor(a) principal: Schiavon, Nicola
Data de Publicação: 2021
Outros Autores: Ding, Yufan, Salvador, Catia, Caldeira, Ana, Angelini, Emma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/31937
https://doi.org/10.3390/cmd2010002
https://doi.org/10.3390/coatings11020209
Resumo: An experimental study was conducted to assess the nature and extent of the biodeterioration of the limestone in the Batalha Monastery in Portugal. Stone fragments covered with microbial biofilms and lichenous crusts were investigated using Optical Microscopy (OM), Low Vacuum Scanning Electron Microscopy with Energy Dispersive Spectroscopy (LV-SEM + EDS), and X-ray micro-Diffractometry (-XRD). Microbial samples were collected from the stone surface, cultured, and analyzed with NGS metagenomic DNA test to classify the bacterial communities associated with the formation of the biofilms. Particulate air pollutants collected on Pall GN-6 paper filters using a cascade impactor were characterized by SEM-EDS + NGS. The results showed that lichens play a major role in biodeterioration by promoting both physical and chemical attack on the limestone substrate via hyphae mechanical penetration along calcite inter-crystalline spaces, the dissolution/leaching of calcite minerals, and the precipitation of secondary minerals such as Ca-oxalates within the stone porosity framework. DNA analyses identified the bacterial communities within the biofilms and their relative abundances. Air quality monitoring results suggest that the microbial population colonizing the monastery limestone could at least partially be derived from the dry and wet deposition of airborne biological particles on the stone surfaces and that S, N, and P-rich air pollutants may have provided nutrients and energy for the bacteria communities, thus indirectly facilitating biofilm formation, the growth of a lichenous crusts, and limestone biodeterioration effects.
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spelling On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, PortugalBiodeteriorationBatalha Monasterylimestone decaylichen microbiomesAn experimental study was conducted to assess the nature and extent of the biodeterioration of the limestone in the Batalha Monastery in Portugal. Stone fragments covered with microbial biofilms and lichenous crusts were investigated using Optical Microscopy (OM), Low Vacuum Scanning Electron Microscopy with Energy Dispersive Spectroscopy (LV-SEM + EDS), and X-ray micro-Diffractometry (-XRD). Microbial samples were collected from the stone surface, cultured, and analyzed with NGS metagenomic DNA test to classify the bacterial communities associated with the formation of the biofilms. Particulate air pollutants collected on Pall GN-6 paper filters using a cascade impactor were characterized by SEM-EDS + NGS. The results showed that lichens play a major role in biodeterioration by promoting both physical and chemical attack on the limestone substrate via hyphae mechanical penetration along calcite inter-crystalline spaces, the dissolution/leaching of calcite minerals, and the precipitation of secondary minerals such as Ca-oxalates within the stone porosity framework. DNA analyses identified the bacterial communities within the biofilms and their relative abundances. Air quality monitoring results suggest that the microbial population colonizing the monastery limestone could at least partially be derived from the dry and wet deposition of airborne biological particles on the stone surfaces and that S, N, and P-rich air pollutants may have provided nutrients and energy for the bacteria communities, thus indirectly facilitating biofilm formation, the growth of a lichenous crusts, and limestone biodeterioration effects.European Commission, H2020-MSCA-ITN-EJD ED-ARCHMAT, GA 766311Coatings2022-04-29T09:25:03Z2022-04-292021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/31937https://doi.org/10.3390/cmd2010002http://hdl.handle.net/10174/31937https://doi.org/10.3390/coatings11020209engYufan Ding, Catia Salvador, Ana Caldeira, Emma Angelini, & Nick Schiavon. (2021). Biodegradation and Microbial Contamination of Limestone Surfaces: An Experimental Study from Batalha Monastery, Portugalschiavon@uevora.ptndndndnd247Schiavon, NicolaDing, YufanSalvador, CatiaCaldeira, AnaAngelini, Emmainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:32:06Zoai:dspace.uevora.pt:10174/31937Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:21:02.620964Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
title On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
spellingShingle On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
Schiavon, Nicola
Biodeterioration
Batalha Monastery
limestone decay
lichen microbiomes
title_short On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
title_full On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
title_fullStr On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
title_full_unstemmed On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
title_sort On the biodiversity and biodeteriogenic activity of microbial communities present in the hypogenic environment of the Escoural Cave, Alentejo, Portugal
author Schiavon, Nicola
author_facet Schiavon, Nicola
Ding, Yufan
Salvador, Catia
Caldeira, Ana
Angelini, Emma
author_role author
author2 Ding, Yufan
Salvador, Catia
Caldeira, Ana
Angelini, Emma
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Schiavon, Nicola
Ding, Yufan
Salvador, Catia
Caldeira, Ana
Angelini, Emma
dc.subject.por.fl_str_mv Biodeterioration
Batalha Monastery
limestone decay
lichen microbiomes
topic Biodeterioration
Batalha Monastery
limestone decay
lichen microbiomes
description An experimental study was conducted to assess the nature and extent of the biodeterioration of the limestone in the Batalha Monastery in Portugal. Stone fragments covered with microbial biofilms and lichenous crusts were investigated using Optical Microscopy (OM), Low Vacuum Scanning Electron Microscopy with Energy Dispersive Spectroscopy (LV-SEM + EDS), and X-ray micro-Diffractometry (-XRD). Microbial samples were collected from the stone surface, cultured, and analyzed with NGS metagenomic DNA test to classify the bacterial communities associated with the formation of the biofilms. Particulate air pollutants collected on Pall GN-6 paper filters using a cascade impactor were characterized by SEM-EDS + NGS. The results showed that lichens play a major role in biodeterioration by promoting both physical and chemical attack on the limestone substrate via hyphae mechanical penetration along calcite inter-crystalline spaces, the dissolution/leaching of calcite minerals, and the precipitation of secondary minerals such as Ca-oxalates within the stone porosity framework. DNA analyses identified the bacterial communities within the biofilms and their relative abundances. Air quality monitoring results suggest that the microbial population colonizing the monastery limestone could at least partially be derived from the dry and wet deposition of airborne biological particles on the stone surfaces and that S, N, and P-rich air pollutants may have provided nutrients and energy for the bacteria communities, thus indirectly facilitating biofilm formation, the growth of a lichenous crusts, and limestone biodeterioration effects.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-01T00:00:00Z
2022-04-29T09:25:03Z
2022-04-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/31937
https://doi.org/10.3390/cmd2010002
http://hdl.handle.net/10174/31937
https://doi.org/10.3390/coatings11020209
url http://hdl.handle.net/10174/31937
https://doi.org/10.3390/cmd2010002
https://doi.org/10.3390/coatings11020209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Yufan Ding, Catia Salvador, Ana Caldeira, Emma Angelini, & Nick Schiavon. (2021). Biodegradation and Microbial Contamination of Limestone Surfaces: An Experimental Study from Batalha Monastery, Portugal
schiavon@uevora.pt
nd
nd
nd
nd
247
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Coatings
publisher.none.fl_str_mv Coatings
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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