Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/20055 |
Resumo: | In this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results in vivo through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as: Dglucose=5.8x10–7 cm2/s and Dglucose=4.4x10–7 cm2/s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the in vivo situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols. |
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Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic ApproachesColorectal cancerEnhanced tissue spectroscopyCancer detectionOptical clearing mechanismsTissue optical clearingIn this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results in vivo through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as: Dglucose=5.8x10–7 cm2/s and Dglucose=4.4x10–7 cm2/s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the in vivo situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols.The authors of the article knew well and communicated with Ekaterina Borisova for many years, especially Valery Tuchin and in recent years Luís Oliveira. We had many joint plans to organize conferences and joint research projects, but COVID-19 mercilessly interrupted our communication. We have lost a great scientist and a person with a huge soul, sociable, but at the same time modest and kind. We will always remember our warm meetings and fruitful work with Ekaterina. This research was supported by the Portuguese grant FCT-UIDB/04730/2020. VVT was supported by grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).Samara UniversityRepositório Científico do Instituto Politécnico do PortoFernandes, LuísSilva, HugoMartins, InêsCarvalho, SóniaCarneiro, IsaHenrique, RuiTuchin, Valery V.Oliveira, Luís2022-02-24T12:14:54Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdftext/plain; charset=utf-8http://hdl.handle.net/10400.22/20055engFernandes, L., Silva, H., Martins, I., Carvalho, S., Carneiro, I., Henrique, R., Tuchin, V., Oliveira, L. M. (2021). Tissue spectroscopy and optical clearing of colorectal mucosa in the pursuit of new cancer diagnostic approaches. J. Biomed. Phot. & Eng., vol. 7, no. 4, 040302. DOI: 10.18287/JBPE21.07.04030210.18287/JBPE21.07.040302info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:14:51Zoai:recipp.ipp.pt:10400.22/20055Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:40:01.875660Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
title |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
spellingShingle |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches Fernandes, Luís Colorectal cancer Enhanced tissue spectroscopy Cancer detection Optical clearing mechanisms Tissue optical clearing |
title_short |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
title_full |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
title_fullStr |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
title_full_unstemmed |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
title_sort |
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches |
author |
Fernandes, Luís |
author_facet |
Fernandes, Luís Silva, Hugo Martins, Inês Carvalho, Sónia Carneiro, Isa Henrique, Rui Tuchin, Valery V. Oliveira, Luís |
author_role |
author |
author2 |
Silva, Hugo Martins, Inês Carvalho, Sónia Carneiro, Isa Henrique, Rui Tuchin, Valery V. Oliveira, Luís |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Fernandes, Luís Silva, Hugo Martins, Inês Carvalho, Sónia Carneiro, Isa Henrique, Rui Tuchin, Valery V. Oliveira, Luís |
dc.subject.por.fl_str_mv |
Colorectal cancer Enhanced tissue spectroscopy Cancer detection Optical clearing mechanisms Tissue optical clearing |
topic |
Colorectal cancer Enhanced tissue spectroscopy Cancer detection Optical clearing mechanisms Tissue optical clearing |
description |
In this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results in vivo through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as: Dglucose=5.8x10–7 cm2/s and Dglucose=4.4x10–7 cm2/s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the in vivo situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-02-24T12:14:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/20055 |
url |
http://hdl.handle.net/10400.22/20055 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes, L., Silva, H., Martins, I., Carvalho, S., Carneiro, I., Henrique, R., Tuchin, V., Oliveira, L. M. (2021). Tissue spectroscopy and optical clearing of colorectal mucosa in the pursuit of new cancer diagnostic approaches. J. Biomed. Phot. & Eng., vol. 7, no. 4, 040302. DOI: 10.18287/JBPE21.07.040302 10.18287/JBPE21.07.040302 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/plain; charset=utf-8 |
dc.publisher.none.fl_str_mv |
Samara University |
publisher.none.fl_str_mv |
Samara University |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131487543492608 |