Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/33289 |
Resumo: | Purpose: Silver nanoparticles (SN) have been studied as antibiofilm agents, to prevent Candida-associated denture stomatitis. Consequently, the aim of this study was to investigate the influence of the intermediate and maturation stages of Candida albicans and Candida glabrata biofilms development in the susceptibility to SN. Methods: SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat, during 5 h, Candida biofilms grown on acrylic surfaces for 24- (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFU) quantification. Results: SN promoted significant reductions (p < 0.05) in the total biomass and in the number of CFUs of Candida biofilms ranging from 23-51.5% and 0.96-1.44-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p > 0.05), when the different stages of biofilm development (24 or 48 h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p < 0.05) was found only for the strain C. albicans 324LA/94. Conclusions: It was concluded that, in general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN regarding. These findings are essential for the deployment of new therapies aimed at preventing denture stomatitis. |
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Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phasesSilver nanoparticlesDenture stomatitisBiofilmsCandida albicansCandida glabrataScience & TechnologyPurpose: Silver nanoparticles (SN) have been studied as antibiofilm agents, to prevent Candida-associated denture stomatitis. Consequently, the aim of this study was to investigate the influence of the intermediate and maturation stages of Candida albicans and Candida glabrata biofilms development in the susceptibility to SN. Methods: SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat, during 5 h, Candida biofilms grown on acrylic surfaces for 24- (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFU) quantification. Results: SN promoted significant reductions (p < 0.05) in the total biomass and in the number of CFUs of Candida biofilms ranging from 23-51.5% and 0.96-1.44-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p > 0.05), when the different stages of biofilm development (24 or 48 h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p < 0.05) was found only for the strain C. albicans 324LA/94. Conclusions: It was concluded that, in general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN regarding. These findings are essential for the deployment of new therapies aimed at preventing denture stomatitis.This study was supported by the Sao Paulo Research Foundation (FAPESP), Brazil, process 2009/15146-5. The authors are indebted to LIEC-CMDMC and INCTMN/FAPESP-CNPq in the name of Andressa Kubo for preparing and characterizing the colloidal suspensions of silver nanoparticles. We also thank Dr. David Williams (Cardiff University, Cardiff, UK) for providing the strain 324LA/94, and George Duchow for the English review.ElsevierUniversidade do MinhoMonteiro, D. R.Takamiya, A. S.Feresin, L. P.Gorup, L. F.Camargo, E. R.Delbem, A. C. B.Henriques, MarianaBarbosa, D. B.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33289eng1883-19581883-195810.1016/j.jpor.2014.07.00425168655http://www.journals.elsevier.com/journal-of-prosthodontic-research/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:16:19Zoai:repositorium.sdum.uminho.pt:1822/33289Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:16:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
title |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
spellingShingle |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases Monteiro, D. R. Silver nanoparticles Denture stomatitis Biofilms Candida albicans Candida glabrata Science & Technology |
title_short |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
title_full |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
title_fullStr |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
title_full_unstemmed |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
title_sort |
Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases |
author |
Monteiro, D. R. |
author_facet |
Monteiro, D. R. Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
author_role |
author |
author2 |
Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Monteiro, D. R. Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
dc.subject.por.fl_str_mv |
Silver nanoparticles Denture stomatitis Biofilms Candida albicans Candida glabrata Science & Technology |
topic |
Silver nanoparticles Denture stomatitis Biofilms Candida albicans Candida glabrata Science & Technology |
description |
Purpose: Silver nanoparticles (SN) have been studied as antibiofilm agents, to prevent Candida-associated denture stomatitis. Consequently, the aim of this study was to investigate the influence of the intermediate and maturation stages of Candida albicans and Candida glabrata biofilms development in the susceptibility to SN. Methods: SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat, during 5 h, Candida biofilms grown on acrylic surfaces for 24- (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFU) quantification. Results: SN promoted significant reductions (p < 0.05) in the total biomass and in the number of CFUs of Candida biofilms ranging from 23-51.5% and 0.96-1.44-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p > 0.05), when the different stages of biofilm development (24 or 48 h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p < 0.05) was found only for the strain C. albicans 324LA/94. Conclusions: It was concluded that, in general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN regarding. These findings are essential for the deployment of new therapies aimed at preventing denture stomatitis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/33289 |
url |
http://hdl.handle.net/1822/33289 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1883-1958 1883-1958 10.1016/j.jpor.2014.07.004 25168655 http://www.journals.elsevier.com/journal-of-prosthodontic-research/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544912529260544 |