Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast

Detalhes bibliográficos
Autor(a) principal: Castro, Mário Rui
Data de Publicação: 2011
Outros Autores: Belo, A.D.F., Afonso, Anabela, Zavattieri, Maria Amely
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/3942
https://doi.org/10.1007/s10725-011-9590-1
Resumo: Juniperus navicularis Gand (= J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious shrub from the Plio-Plistocene transition sands of the west Portuguese coast line. It grows in sole or mixed stands along with Pinus pinea and P. pinaster and, more rarely, with Quercus lusitanica. In order to reduce fire incidence in these Mediterranean mixed stands, forest management practices of mechanical or chemical treatments, have drastically reduced J. navicularis populations. In addition, the fast expansion of touristic areas along with a low seed germination rate further endangers this species. In this study we developed a protocol for micropropagation of adult J. navicularis. Microcuttings were obtained from lateral and apical twigs of both female and male mature plants, and used as explants. Microcuttings with axillary buds were grown on different media and plant growth regulators combinations. Developmental parameters were evaluated to define the best medium and plant growth regulator concentration for shoot bud induction during the multiplication cycles. Olive Medium and Gupta and Durzan medium, both supplemented with 0.45 µM 6-benzyl-amino-purine, were the most favourable of all combinations tested achieving more than 3 new shoots per explant. The highest rooting (60%) was obtained in microshoots cultured in Olive Medium when supplemented with indole-3-butyric acid at 12.3 µM. With this multiplication protocol, it was possible to obtain more than 500 shoots from the initial 20, after three multiplication cycles in 3 months.
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spelling Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coastConiferConservationCupressaceaeMicroshootsRootingTissue cultureJuniperus navicularis Gand (= J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious shrub from the Plio-Plistocene transition sands of the west Portuguese coast line. It grows in sole or mixed stands along with Pinus pinea and P. pinaster and, more rarely, with Quercus lusitanica. In order to reduce fire incidence in these Mediterranean mixed stands, forest management practices of mechanical or chemical treatments, have drastically reduced J. navicularis populations. In addition, the fast expansion of touristic areas along with a low seed germination rate further endangers this species. In this study we developed a protocol for micropropagation of adult J. navicularis. Microcuttings were obtained from lateral and apical twigs of both female and male mature plants, and used as explants. Microcuttings with axillary buds were grown on different media and plant growth regulators combinations. Developmental parameters were evaluated to define the best medium and plant growth regulator concentration for shoot bud induction during the multiplication cycles. Olive Medium and Gupta and Durzan medium, both supplemented with 0.45 µM 6-benzyl-amino-purine, were the most favourable of all combinations tested achieving more than 3 new shoots per explant. The highest rooting (60%) was obtained in microshoots cultured in Olive Medium when supplemented with indole-3-butyric acid at 12.3 µM. With this multiplication protocol, it was possible to obtain more than 500 shoots from the initial 20, after three multiplication cycles in 3 months.Springer2012-01-20T14:46:50Z2012-01-202011-10-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/3942http://hdl.handle.net/10174/3942https://doi.org/10.1007/s10725-011-9590-1engCastro, Mário R; Belo, Anabela F; Afonso, Anabela; Zavattieri, Maria A. 2011. "Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast", Plant Growth Regulation 65, 2: 223 - 230. doi: 10.1007/s10725-011-9590-1ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científicamrcbcastro@gmail.comafb@uevora.ptaafonso@uevora.ptzavattieri@uevora.pt528Castro, Mário RuiBelo, A.D.F.Afonso, AnabelaZavattieri, Maria Amelyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:26Zoai:dspace.uevora.pt:10174/3942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:16.411209Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
title Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
spellingShingle Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
Castro, Mário Rui
Conifer
Conservation
Cupressaceae
Microshoots
Rooting
Tissue culture
title_short Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
title_full Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
title_fullStr Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
title_full_unstemmed Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
title_sort Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast
author Castro, Mário Rui
author_facet Castro, Mário Rui
Belo, A.D.F.
Afonso, Anabela
Zavattieri, Maria Amely
author_role author
author2 Belo, A.D.F.
Afonso, Anabela
Zavattieri, Maria Amely
author2_role author
author
author
dc.contributor.author.fl_str_mv Castro, Mário Rui
Belo, A.D.F.
Afonso, Anabela
Zavattieri, Maria Amely
dc.subject.por.fl_str_mv Conifer
Conservation
Cupressaceae
Microshoots
Rooting
Tissue culture
topic Conifer
Conservation
Cupressaceae
Microshoots
Rooting
Tissue culture
description Juniperus navicularis Gand (= J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious shrub from the Plio-Plistocene transition sands of the west Portuguese coast line. It grows in sole or mixed stands along with Pinus pinea and P. pinaster and, more rarely, with Quercus lusitanica. In order to reduce fire incidence in these Mediterranean mixed stands, forest management practices of mechanical or chemical treatments, have drastically reduced J. navicularis populations. In addition, the fast expansion of touristic areas along with a low seed germination rate further endangers this species. In this study we developed a protocol for micropropagation of adult J. navicularis. Microcuttings were obtained from lateral and apical twigs of both female and male mature plants, and used as explants. Microcuttings with axillary buds were grown on different media and plant growth regulators combinations. Developmental parameters were evaluated to define the best medium and plant growth regulator concentration for shoot bud induction during the multiplication cycles. Olive Medium and Gupta and Durzan medium, both supplemented with 0.45 µM 6-benzyl-amino-purine, were the most favourable of all combinations tested achieving more than 3 new shoots per explant. The highest rooting (60%) was obtained in microshoots cultured in Olive Medium when supplemented with indole-3-butyric acid at 12.3 µM. With this multiplication protocol, it was possible to obtain more than 500 shoots from the initial 20, after three multiplication cycles in 3 months.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-21T00:00:00Z
2012-01-20T14:46:50Z
2012-01-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/3942
http://hdl.handle.net/10174/3942
https://doi.org/10.1007/s10725-011-9590-1
url http://hdl.handle.net/10174/3942
https://doi.org/10.1007/s10725-011-9590-1
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro, Mário R; Belo, Anabela F; Afonso, Anabela; Zavattieri, Maria A. 2011. "Micropropagation of Juniperus navicularis, an endemic and rare species from Portugal SW coast", Plant Growth Regulation 65, 2: 223 - 230. doi: 10.1007/s10725-011-9590-1
ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
mrcbcastro@gmail.com
afb@uevora.pt
aafonso@uevora.pt
zavattieri@uevora.pt
528
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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