Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients

Detalhes bibliográficos
Autor(a) principal: Araújo, João R.
Data de Publicação: 2023
Outros Autores: Serafim, Thainá, Ismael, Shámila, Calhau, Conceição, Faria, Ana, Teixeira, Diana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/159715
Resumo: Funding Information: Funding: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização - COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia (grant number 268_596883842), IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020) and CHRC (UIDP/04923/2020 and UIDB/04923/2020). Also supported by BIOCODEX and project RISE - LA/P/0053/2020. Publisher Copyright: © 2023 The Authors
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spelling Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 PatientsAlkaline PhosphataseCoronavirus Disease 2019GutInflammationMedicine (miscellaneous)GastroenterologyHepatologyFunding Information: Funding: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização - COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia (grant number 268_596883842), IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020) and CHRC (UIDP/04923/2020 and UIDB/04923/2020). Also supported by BIOCODEX and project RISE - LA/P/0053/2020. Publisher Copyright: © 2023 The AuthorsBackground & Aims: Although gut inflammation and dysbiosis have been implicated in the pathophysiology of severe cases of coronavirus disease 2019 (COVID-19), the role of intestinal anti-inflammatory enzymes, such as alkaline phosphatase, is still underexplored. Therefore, the aim of this study was to compare intestinal alkaline phosphatase (iALP) activity and its proinflammatory substrate - bacterial lipopolysaccharide (LPS) - concentration between mild-to-moderate and severe COVID-19 patients. Methods: Stool samples collected from 53 mild-to-moderate and 57 severe adult COVID-19 patients, previously enrolled in a national multicentre cross-sectional study (NCT04355741), were analysed for iALP activity and LPS concentration. Results: iALP activity decreased by 40% in severe compared to mild-to-moderate COVID-19 patients (median [interquartile range] of 120.6 [25.2–593.1] nmol pNP/min/g of protein vs 202.8 [102.1–676.1] nmol pNP/min/g of protein; P = .04) after adjustment for clinical and gut microbiota parameters. Regarding fecal LPS, its concentration was found to be decreased in severe patients (mean ± standard error of mean of 18,118 ± 1225 EU/g of feces vs 22,508 ± 1203 EU/g of feces; P = .01), although this parameter did not correlate with plasma levels of C-reactive protein (P = .08), a sensitive biomarker of systemic inflammation. In contrast, fecal ALP activity / LPS concentration ratio, an indicator of iALP efficiency, was found to be increased in severe compared to mild-to-moderate COVID-19 patients (P = .04). Conclusion: Changes in iALP kinetic parameters found in severe COVID-19 patients may represent a potential mechanism to counterbalance alterations in gut homeostasis (eg inflammation and dysbiosis) associated with COVID-19 severity.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)Comprehensive Health Research Centre (CHRC) - pólo NMSRUNAraújo, João R.Serafim, ThaináIsmael, ShámilaCalhau, ConceiçãoFaria, AnaTeixeira, Diana2023-11-08T22:08:27Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/159715eng2772-5723PURE: 74055753https://doi.org/10.1016/j.gastha.2023.07.003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:42:09Zoai:run.unl.pt:10362/159715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:40.190424Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
title Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
spellingShingle Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
Araújo, João R.
Alkaline Phosphatase
Coronavirus Disease 2019
Gut
Inflammation
Medicine (miscellaneous)
Gastroenterology
Hepatology
title_short Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
title_full Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
title_fullStr Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
title_full_unstemmed Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
title_sort Intestinal Alkaline Phosphatase Activity and Efficiency Are Altered in Severe COVID-19 Patients
author Araújo, João R.
author_facet Araújo, João R.
Serafim, Thainá
Ismael, Shámila
Calhau, Conceição
Faria, Ana
Teixeira, Diana
author_role author
author2 Serafim, Thainá
Ismael, Shámila
Calhau, Conceição
Faria, Ana
Teixeira, Diana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)
Comprehensive Health Research Centre (CHRC) - pólo NMS
RUN
dc.contributor.author.fl_str_mv Araújo, João R.
Serafim, Thainá
Ismael, Shámila
Calhau, Conceição
Faria, Ana
Teixeira, Diana
dc.subject.por.fl_str_mv Alkaline Phosphatase
Coronavirus Disease 2019
Gut
Inflammation
Medicine (miscellaneous)
Gastroenterology
Hepatology
topic Alkaline Phosphatase
Coronavirus Disease 2019
Gut
Inflammation
Medicine (miscellaneous)
Gastroenterology
Hepatology
description Funding Information: Funding: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização - COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia (grant number 268_596883842), IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020) and CHRC (UIDP/04923/2020 and UIDB/04923/2020). Also supported by BIOCODEX and project RISE - LA/P/0053/2020. Publisher Copyright: © 2023 The Authors
publishDate 2023
dc.date.none.fl_str_mv 2023-11-08T22:08:27Z
2023-01
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/159715
url http://hdl.handle.net/10362/159715
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2772-5723
PURE: 74055753
https://doi.org/10.1016/j.gastha.2023.07.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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